カタログ検索結果 詳細情報

Medium Detail Information

Medium No.	387
Medium	PE Medium
Composition
K2HPO4 0.39 g   KH2PO4 0.38 g   NaCl 0.12 g   CaCl2·2H2O 0.015 g   MgSO4·7H2O 0.12 g   (NH4)2SO4 0.5 g   Sodium glutamate 0.5 g   Sodium succinate 0.5 g   Sodium acetate 0.5 g   Yeast extract 0.5 g   Casamino acids 0.5 g   Na2S2O3·5H2O 0.5 g   Trace element solution* 10 ml   Vitamin solution** 10 ml   Agar (if needed) 15 g   Distilled water 1 L   pH 7.5       Add the vitamin solution aseptically to the cooled autoclaved medium.                   *Trace element solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NaCl 1 g       NiCl2·6H2O 0.12 g       Co(NO3)2·6H2O 0.015 g       Distilled water 1 L     First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.                   **Vitamin solution       3Na-EDTA 200 mg       Nicotinic acid 100 mg       Thiamine-HCl 100 mg       Biotin 5 mg       p-Aminobenzoic acid 50 mg       Vitamin B12 1 mg       Ca-pantothenate 50 mg       Pyridoxine-HCl 50 mg       Folic acid 50 mg       Distilled water 1 L     Adjust pH to 7 with NaOH. Sterilized by filtration.
Comment