| K2HPO4 | 0.39 | g | | KH2PO4 | 0.38 | g | | NaCl | 0.12 | g | | CaCl2·2H2O | 0.015 | g | | MgSO4·7H2O | 0.12 | g | | (NH4)2SO4 | 0.5 | g | | Sodium glutamate | 0.5 | g | | Sodium succinate | 0.5 | g | | Sodium acetate | 0.5 | g | | Yeast extract | 0.5 | g | | Casamino acids | 0.5 | g | | Na2S2O3·5H2O | 0.5 | g | | Trace element solution* | 10 | ml | | Vitamin solution** | 10 | ml | | Agar (if needed) | 15 | g | | Distilled water | 1 | L | | pH 7.5 | | | | | Add the vitamin solution aseptically to the cooled autoclaved medium. | | | | | | | | | | *Trace element solution | | | | Nitrilotriacetic acid (NTA) | 12.8 | g | | | | FeCl3·6H2O | 1.35 | g | | | | MnCl2·4H2O | 0.1 | g | | | | CoCl2·6H2O | 0.024 | g | | | | CaCl2·2H2O | 0.1 | g | | | | ZnCl2 | 0.1 | g | | | | CuCl2·2H2O | 0.025 | g | | | | H3BO3 | 0.01 | g | | | | Na2MoO4·2H2O | 0.024 | g | | | | NaCl | 1 | g | | | | NiCl2·6H2O | 0.12 | g | | | | Co(NO3)2·6H2O | 0.015 | g | | | | Distilled water | 1 | L | | | | First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0. | | | | | | | | | | **Vitamin solution | | | | 3Na-EDTA | 200 | mg | | | | Nicotinic acid | 100 | mg | | | | Thiamine-HCl | 100 | mg | | | | Biotin | 5 | mg | | | | p-Aminobenzoic acid | 50 | mg | | | | Vitamin B12 | 1 | mg | | | | Ca-pantothenate | 50 | mg | | | | Pyridoxine-HCl | 50 | mg | | | | Folic acid | 50 | mg | | | | Distilled water | 1 | L | | | | Adjust pH to 7 with NaOH. Sterilized by filtration. |
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