Search Results Detail

Medium Detail Information

Medium No.
393
Medium
Archaeoglobus profundus medium
Composition
 KCl0.34g
 MgCl2·6H2O4g
 MgSO4·7H2O3.45g
 NH4Cl0.25g
 CaCl2·2H2O0.14g
 K2HPO40.14g
 NaCl18g
 NaHCO31g
 Sodium acetate1g
 Na2SO42.7g
 Bacto Yeast Extract (Difco)0.5g
 Fe(NH4)2(SO4)2·7H2O2mg
 Trace element solution*10ml
 Resazurin1mg
 Na2S·9H2O0.5g
 Distilled water1L
  Dissolve ingredients except bicarbonate and sulfide, bring to boil for few minutes, then cool quickly to room temperature while gassing with H2/CO2 (80/20), add sodium bicarbonate and adjust pH to 6.5. Distribute in serum bottles (20 ml per 100 ml bottle) under H2/CO2, seal, then autoclave. Before use, reduce the medium with sodium sulfide from anaerobic sterile neutral stock solution, and pressurize bottles up to 2 bar overpressure.
       
  *Trace element solution
   Nitrilotriacetic acid1.5g
   MgSO4·7H2O3g
   MnSO4·2H2O0.5g
   NaCl1g
   FeSO4·7H2O0.1g
   CoSO4·7H2O0.18g
   CaCl2·2H2O0.1g
   ZnSO4·7H2O0.18g
   CuSO4·5H2O0.01g
   KAl(SO4)2·12H2O0.02g
   H3BO30.01g
   Na2MoO4·2H2O0.01g
   NiCl2·6H2O0.025g
   Na2SeO3·5H2O0.3mg
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).
Comment