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Medium Detail Information

Medium No.
 397
Medium
 VY/2 Agar
Composition
 Baker's yeast5g
 CaCl2·2H2O1.36g
 Vitamin B120.5mg
 Bacto Agar (Difco)15g
 Distilled water1L
  Sterilized vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50℃ (use pH-indicator paper).
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