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Medium Detail Information

Medium No.
400
Medium
M1D Medium
Composition
 Bacto PPLO Broth w/o CV (Difco)2g
 Bacto Tryptone (Difco)1g
 Bacto Peptone (Difco)0.8g
 Glucose0.1g
 Fructose0.1g
 Sucrose1g
 Sorbitol7.5g
 Phenol red6mg
  Dissolve above in 80 ml of water and adjust to pH 7.8. Bring to 90 ml with water. Autoclave and cool to room temperature. Add the following under sterile conditions: Schneider's Drosophila medium (Invitorogen, Gibco) 160 ml, Fetal bovine serum (heat-inactivated) 50 ml, Penicillin G (100,000 U/ml) 2.5 ml.
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