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Medium Detail Information

Medium No.	809
Medium
Composition
Glucose 2.2 g   Bacto Yeast Extract (Difco) 2.3 g   KH2PO4 0.15 g   NH4Cl 0.5 g   MgCl2·6H2O 0.2 g   CaCl2·2H2O 0.15 g   NaHCO3 2.5 g   Na2S·9H2O 0.3 g   Cysteine-HCl 0.3 g   Trace elements solution* 1 ml   Vitamin solution** 1 ml   Resazurin solution (1 mg/ml) 1 ml   Distilled water 1 L   Adjust pH to 7.0 at 25℃       Prepare the medium anaerobically under a N2/CO2 (80/20) atmosphere. Neutralized substrates were added to the vials from stock solutions prior to inoculation.                   *Trace elements solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NiCl2·6H2O 0.12 g       Na2SeO3·5H2O 0.026 g       Na2WO4·H2O 0.003 g       Distilled water 1 L                   **Vitamin solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       Vitamin B12 0.1 mg       p-Aminobenzoic acid 5 mg       Lipoic acid 5 mg       Distilled water 1 L
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