| Glucose | 2.2 | g |
| Bacto Yeast Extract (Difco) | 2.3 | g |
| KH2PO4 | 0.15 | g |
| NH4Cl | 0.5 | g |
| MgCl2·6H2O | 0.2 | g |
| CaCl2·2H2O | 0.15 | g |
| NaHCO3 | 2.5 | g |
| Na2S·9H2O | 0.3 | g |
| Cysteine-HCl | 0.3 | g |
| Trace elements solution* | 1 | ml |
| Vitamin solution** | 1 | ml |
| Resazurin solution (1 mg/ml) | 1 | ml |
| Distilled water | 1 | L |
| Adjust pH to 7.0 at 25℃ | |
|
| | Prepare the medium anaerobically under a N2/CO2 (80/20) atmosphere. Neutralized substrates were added to the vials from stock solutions prior to inoculation. |
| | | | | | |
| | *Trace elements solution |
| | | Nitrilotriacetic acid (NTA) | 12.8 | g |
| | | FeCl3·6H2O | 1.35 | g |
| | | MnCl2·4H2O | 0.1 | g |
| | | CoCl2·6H2O | 0.024 | g |
| | | CaCl2·2H2O | 0.1 | g |
| | | ZnCl2 | 0.1 | g |
| | | CuCl2·2H2O | 0.025 | g |
| | | H3BO3 | 0.01 | g |
| | | Na2MoO4·2H2O | 0.024 | g |
| | | NiCl2·6H2O | 0.12 | g |
| | | Na2SeO3·5H2O | 0.026 | g |
| | | Na2WO4·H2O | 0.003 | g |
| | | Distilled water | 1 | L |
| | | | | | |
| | **Vitamin solution |
| | | Biotin | 2 | mg |
| | | Folic acid | 2 | mg |
| | | Pyridoxine-HCl | 10 | mg |
| | | Thiamine-HCl | 5 | mg |
| | | Riboflavin | 5 | mg |
| | | Nicotinic acid | 5 | mg |
| | | Ca-pantothenate | 5 | mg |
| | | Vitamin B12 | 0.1 | mg |
| | | p-Aminobenzoic acid | 5 | mg |
| | | Lipoic acid | 5 | mg |
| | | Distilled water | 1 | L |