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Medium Detail Information

Medium No.
833
Medium
Pyrodictium occultum Medium
Composition
 NaCl13.85g
 MgSO4·7H2O3.5g
 MgCl2·6H2O2.75g
 KCl0.325g
 NaBr0.05g
 H3BO315mg
 SrCl2·6H2O7.5mg
 KI0.05mg
 CaCl20.75g
 KH2PO40.5g
 (NH4)2Ni(SO4)2·6H2O2mg
 Trace minerals*10ml
 Bacto Yeast Extract (Difco)2g
 Citric acid5mg
 Na2S2O3·5H2O2.5g
 Sulfur (powder)30g
 Resazurin1mg
 Na2S·9H2O0.3g
 Distilled water1L
  Mix ingredients except sulfur and Na2S·9H2O, adjust pH to 5.5 with H2SO4, and autoclave under H2/CO2 (80/20) conditions. Separately autoclave neutralize Na2S·9H2O solution. Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each of 3 successive days. Aseptically and anaerobically add sterile sulfur to the medium under a H2/CO2 (80/20) atmosphere. Prior to inoculation, add Na2S·9H2O solution, and readjust pH to 5.5 if necessary. Pressurize inoculated bottles to 200 kPa with H2/CO2 (80/20).
       
  *Trace minerals
   Nitrilotriacetic acid1.5g
   MgSO4·7H2O3g
   MnSO4·xH2O0.5g
   NaCl1g
   FeSO4·7H2O0.1g
   CoSO4·7H200.1g
   CaCl2·2H2O0.1g
   ZnSO4·7H2O0.1g
   CuSO4·5H2O0.01g
   AlK(SO4)20.01g
   H3BO30.01g
   Na2MoO4·2H2O0.01g
   Distilled water1L
  Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
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