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Medium No.	846
Medium	BYG Medium (for Acidiphilium multivorum)
Composition
Solution A* 500 ml   Solution B** 500 ml     Agar solid medium of BYG contained 2% agar. In order to prevent the hydrolysis of agar and glucose by autoclaving at low pH condition, Solutions A and B are autoclaved separately and combined before solidified (50-60℃).                   *Solution A       (NH4)2SO4 2 g       KCl 0.1 g       KH2PO4 0.5 g       MgSO4·7H2O 0.5 g       Ca(NO3)2·4H2O 0.01 g       Yeast extract 0.1 g       Distilled water 500 ml   Adjust pH to 3.5 with 1 N H2SO4                     **Solution B       Glucose 1 g       Agar (if needed) 20 g       Distilled water 500 ml
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