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Medium Detail Information

Medium No.
862
Medium
Sulfurisphaera Medium (pH 5.0)
Composition
 (NH4)2SO41.3g
 KH2PO40.28g
 MgSO4·7H2O0.25g
 CaCl2·2H2O0.07g
 FeCl3·6H2O0.02g
 MnCl2·4H2O1.8mg
 Na2B4O7·10H2O4.5mg
 ZnSO4·7H2O0.22mg
 CuCl2·2H2O0.05mg
 Na2MoO4·2H2O0.03mg
 VOSO4·nH2O0.03mg
 CoSO4·7H2O0.01mg
 Bacto Yeast Extract (Difco)1g
 Distilled water1L
 pH 5.0 
  Mix ingredients except Bacto Yeast Extract, adjust pH to 5.0 with H2SO4, and autoclave at 121°C for 15 min. Separately autoclave Bacto Yeast Extract as a 10% solution. Prior to inoculation, add Bacto Yeast Extract solution.
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