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Medium No.	868
Medium	1/5 Marine Broth + 0.5% Sodium Pyruvate
Composition
Bacto Marine Broth 2216 (Difco) 7.5 g   Sodium pyruvate 5 g   Agar (if needed) 15 g   Sea water* 800 ml   Distilled water 200 ml   pH 7.6                     *Filtered, aged seawater or artificial seawater (Daigo's Artificial Seawater SP**)                   **Wako Pure Chemical Ind., Ltd., Osaka, Japan.
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