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Medium Detail Information

Medium No.
881
Medium
GYP medium (pH 6)
Composition
 Glucose10g
 Bacto Yeast Extract (Difco)10g
 Hipolypepton*5g
 Sodium acetate2g
 Tween 80 solution**10ml
 Salts solution***5ml
 Distilled water1L
 pH 6.0 
  Add 5 g/L CaCO3 and 15 g/L agar after the pH is adjusted for the solid medium.
       
  *Wako Pure Chemical Ind., Ltd., Osaka, Japan.
       
  **Tween 80 solution
   Tween 8050g
   Distilled water1L
       
  ***Salts solution
   MgSO4·7H2O40g
   MnSO4·4H2O2g
   FeSO4·7H2O2g
   NaCl2g
   Distilled water1L
  Add a drop of 12 N HCl to 500 ml of the salts solution.
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