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Medium Detail Information

Medium No.
897
Medium
Methanothrix Medium
Composition
  Sodium acetate 6.56 g
  NH4Cl 1 g
  KH2PO4 0.3 g
  NaCl 0.6 g
  MgCl2·6H2O 0.1 g
  CaCl2·2H2O 0.08 g
  Trace elements solution* 5 ml
  Vitamin solution** 10 ml
  Resazurin 1 mg
  NaHCO3 3.5 g
  Cysteine-HCl 0.5 g
  Na2S·9H2O 0.5 g
  Distilled water 1 L
 
    Mix ingredients except for vitamin solution, cysteine-HCl and Na2S·9H2O, and autoclave under N2/CO2 (80/20) condition. Separately autoclave cysteine-HCl and Na2S·9H2O as a 5% solution under a N2 atmosphere. Aseptically and anaerobically add filter-sterile vitamin, cysteine-HCl and Na2S·9H2O solutions.
             
    *Trace elements solution
      Nitrilotriacetic acid (NTA) 4.1 g
      FeCl3·6H2O 0.36 g
      MnCl2·4H2O 0.09 g
      CoCl2·6H2O 0.15 g
      CaCl2·2H2O 0.018 g
      ZnCl2 0.09 g
      H3BO3 0.017 g
      KAl(SO4)2·12H2O 0.02 g
      Na2MoO4·2H2O 0.009 g
      NiCl2·6H2O 0.018 g
      Na2SeO3 1.6 mg
      Na2WO4 1.6 mg
      Distilled water 1 L
 
    First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
             
    **Vitamin solution
      Biotin 2 mg
      Folic acid 2 mg
      Pyridoxine-HCl 10 mg
      Thiamine-HCl 5 mg
      Riboflavin 5 mg
      Nicotinic acid 5 mg
      Ca-pantothenate 5 mg
      p-Aminobenzoic acid 1 mg
      Vitamin B12 0.01 mg
      Distilled water 1 L
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