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Medium Detail Information

Medium No.
897
Medium
Methanothrix Medium
Composition
 Sodium acetate6.56g
 NH4Cl1g
 KH2PO40.3g
 NaCl0.6g
 MgCl2·6H2O0.1g
 CaCl2·2H2O0.08g
 Trace elements solution*5ml
 Vitamin solution**10ml
 Resazurin1mg
 NaHCO33.5g
 Cysteine-HCl0.5g
 Na2S·9H2O0.5g
 Distilled water1L
  Mix ingredients except for vitamin solution, cysteine-HCl and Na2S·9H2O, and autoclave under N2/CO2 (80/20) condition. Separately autoclave cysteine-HCl and Na2S·9H2O as a 5% solution under a N2 atmosphere. Aseptically and anaerobically add filter-sterile vitamin, cysteine-HCl and Na2S·9H2O solutions.
       
  *Trace elements solution
   Nitrilotriacetic acid (NTA)4.1g
   FeCl3·6H2O0.36g
   MnCl2·4H2O0.09g
   CoCl2·6H2O0.15g
   CaCl2·2H2O0.018g
   ZnCl20.09g
   H3BO30.017g
   KAl(SO4)2·12H2O0.02g
   Na2MoO4·2H2O0.009g
   NiCl2·6H2O0.018g
   Na2SeO31.6mg
   Na2WO41.6mg
   KAl(SO4)2·12H2O8mg
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
       
  **Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
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