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Medium Detail Information

Medium No.	899
Medium	GYP medium
Composition
Glucose 10 g   Bacto Yeast Extract (Difco) 10 g   Hipolypepton* 5 g   Sodium acetate 2 g   Tween 80 solution** 10 ml   Salts solution*** 5 ml   Distilled water 1 L   pH 6.8       Add 5 g/L CaCO3 and 15 g/L agar after the pH is adjusted for the solid medium.                   *Wako Pure Chemical Ind., Ltd., Osaka, Japan.                   **Tween 80 solution       Tween 80 50 mg       Distilled Water 1 ml                   ***Salts solution       MgSO4·7H2O 40 mg       MnSO4·4H2O 2 mg       FeSO4·7H2O 2 mg       NaCl 2 mg       Distilled Water 1 ml     Add a drop of 12 N HCl to 500 ml of the salts solution.
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