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Medium Detail Information

Medium No.
900
Medium
Thermanaeromonas Medium
Composition
 K2HPO40.78g
 KH2PO40.75g
 3Na-EDTA0.04g
 FeSO4·7H2O0.01g
 MgSO4·7H2O0.25g
 CaCl2·2H2O0.03g
 NaCl0.2g
 NH4Cl0.5g
 NaHCO35g
 Na2S2O30.8g
 Glucose0.5g
 Bacto Yeast Extract (Difco)0.5g
 Vitamin solution*10ml
 Trace element solution**10ml
 Cysteine-HCl0.5g
 Distilled water1L
  Mix ingredients except vitamin solution and cysteine-HCl. Dispense the medium into suitable culture vessels under a stream of N2/CO2 (80/20), seal with butyl rubber stoppers and autoclave. Separately autoclave 5% cysteine-HCl solution under a N2 atmosphere. Sterilize vitamin solution by filtration. Prior to inoculation, aseptically add vitamin and cysteine-HCl solutions.
       
  *Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   Vitamin B120.1mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
       
  **Trace element solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO3·5H2O0.026g
   Distilled water1L
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