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Medium Detail Information

Medium No.
923
Medium
Hydrogenobacter medium
Composition
 KP buffer*10ml
 MgCl2·6H2O0.75g
 CaCl2·2H2O0.15g
 NH4Cl0.54g
 Na2S2O33.2g
 Bacto Yeast Extract (Difco)0.1g
 Peptone (BBL)1g
 Glucose0.2g
 Sodium succinate0.2g
 Trace elements solution**2ml
 Vitamin solution***2ml
 Na2CO30.5g
 Distilled water1L
  Mix ingredients except KP buffer and vitamin solution. Dispense the medium into suitable culture vessels under a stream of H2/CO2/O2 (75/20/5) and seal with butyl rubber stoppers. Separately autoclave KP buffer under a N2 atmosphere. Sterilize vitamin solution by filtration. Prior to inoculation, aseptically add KP buffer and vitamin solution. Pressurize the inoculated vessels to 150 kPa with H2/CO2/O2.
       
  *KP buffer
   KH2PO4119g
   K2HPO421g
   Distilled water1L
  Autoclaved under N2.
       
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   KAl(SO4)2·12H2O0.02g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
       
  ***Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
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