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Medium Detail Information

Medium No.
933
Medium
M medium (EBR46, EBR599)
Composition
 KH2PO40.4g
 K2HPO4·3H2O0.4g
 NH4Cl1g
 MgCl2·6H2O0.1g
 Yeast extract0.2g
 NaHCO36g
 Cysteine-HCl0.5g
 Na2S·9H2O0.25g
 Resazurin1mg
 Bacto Tryptone (Difco)10g
 Vitamin solution*10ml
 Mineral solution**10ml
 Distilled water1L
 pH 7.5 
  Mix ingredients except NaHCO3, cysteine-HCl, Na2S·9H2O and vitamin solution, distribute the medium into culture vessels under a stream of nitrogen gas, and seal with butyl rubber stoppers. After autoclaving (121°C, 15 min), aseptically inject other ingredients. The pH is adjusted to 7.5 by adding of sterile 0.6 N H2SO4 with a syringe without opening the vessel.
       
  *Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   Vitamin B121mg
   p-Aminobenzoic acid5mg
   Lipoic acid5mg
   Distilled water1L
       
  **Mineral solution
   Nitrilotriacetic acid (NTA)4.5g
   FeCl2·4H2O0.4g
   CoCl2·6H2O0.12g
   AlK(SO4)20.01g
   NaCl1g
   CaCl20.02g
   Na2MoO4·2H2O0.01g
   MnCl2·4H2O0.1g
   ZnCl20.1g
   H3BO30.01g
   CuSO4·5H2O0.01g
   NiCl2·6H2O0.02g
   Distilled water1L
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