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Medium No.	972
Medium	Nutrient Broth + 5% NaCl (pH 10.0)
Composition
Nutrient Broth (OXOID) 13 g   NaCl 50 g   Sodium-sesquicarbonate solution* 100 ml   Distilled water 900 ml   Agar (if needed) 15 g                   *Sodium-sesquicarbonate solution       NaHCO3 4.2 g       Na2CO3 (anhydrous) 5.3 g       Distilled water 100 ml     Sterilize separately and add to other autoclaved ingredients. Check final pH to be about 10.0.
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