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Medium Detail Information

Medium No.
975
Medium
Thermus Medium
Composition
 Bacto Yeast Extract (Difco)1g
 Bacto Tryptone (Difco)1g
 CaSO4·2H2O0.06g
 MgSO4·7H2O0.1g
 NaCl0.008g
 KNO30.1g
 NaNO30.69g
 Na2HPO40.11g
 Trace elements solution*10ml
 Agar (if needed)20g
 Distilled water1L
 Adjust pH to 7.5 with NaOH 
       
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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