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Medium Detail Information

Medium No.	979
Medium	Clostridium thermocellum Medium (D58 Medium)
Composition
(NH4)2SO4 1.3 g   MgCl2·6H2O 2.6 g   KH2PO4 1.43 g   K2HPO4·3H2O 7.2 g   CaCl2·2H2O 0.13 g   Sodium glycerophosphate 6 g   FeSO4·7H2O 1.1 mg   Glutathione 0.25 g   Yeast extract 4.5 g   Resazurin 1 mg   Cellulose (Avicel or MN 300) 10 g   or Cellobiose 5 g   Distilled water 1 L   pH 7.0-7.2       Mix ingredients except for cellobiose and autoclave under N2/CO2 (80/20) condition. Aseptically and anaerobically add filter-sterile 10% cellobiose solution before inoculation.
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