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Medium Detail Information

Medium No.
979
Medium
Clostridium thermocellum Medium (D58 Medium)
Composition
 (NH4)2SO41.3g
 MgCl2·6H2O2.6g
 KH2PO41.43g
 K2HPO4·3H2O7.2g
 CaCl2·2H2O0.13g
 Sodium glycerophosphate6g
 FeSO4·7H2O1.1mg
 Glutathione0.25g
 Yeast extract4.5g
 Resazurin1mg
 Cellulose (Avicel or MN 300)10g
 or Cellobiose5g
 Distilled water1L
 pH 7.0-7.2 
  Mix ingredients except for cellobiose and autoclave under N2/CO2 (80/20) condition. Aseptically and anaerobically add filter-sterile 10% cellobiose solution before inoculation.
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