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Medium No.	981
Medium	Verrucomicrobium Medium
Composition
Peptone 0.25 g   Yeast extract 0.25 g   Glucose 0.25 g   Vitamin solution* 10 ml   Hutner's basal salts** 20 ml   0.1 M Tris-HCl (pH 7.5) 50 ml   Artificial sea water*** 250 ml   Distilled water 670 ml     Autoclave at 121°C for 15 min.                   *Vitamin solution       Vitamin B12 0.1 mg       Biotin 2 mg       Thiamine-HCl 5 mg       Ca-pantothenate 5 mg       Folic acid 2 mg       Riboflavin 5 mg       Nicotinamide 5 mg       Distilled water 1 L                   **Hutner's basal salts       Nitrilotriacetic acid (NTA) 10 g       MgSO4·7H2O 29.7 g       CaCl2·2H2O 3.34 g       (NH4)6MoO7O24·4H2O 9.25 mg       FeSO4·7H2O 99 mg       Metal salt solution 44**** 50 ml       Distilled water 950 ml                   ***Artificial sea water       NaCl 23.477 g       Na2SO4 3.917 g       MgCl2·6H2O 4.981 g       CaCl2 1.102 g       NaHCO3 192 mg       KCl 664 mg       KBr 6 mg       H3BO3 26 mg       SrCl2 24 mg       NaF 3 mg       Distilled water 1 L                   ****Metal salt solution 44       Na-EDTA 250 mg       ZnSO4·7H2O 1095 mg       FeSO4·7H2O 500 mg       MnSO4·H2O 154 mg       CuSO4·5H2O 39.2 mg       Co(NO3)2·6H2O 24.8 mg       Na2B4O7·10H2O 17.7 mg       Distilled water 1 L     Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. Store in the dark and cold (5℃).
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