Search Results Detail

Medium Detail Information

Medium No.
988
Medium
TYG medium
Composition
 Trypticase Peptone (BBL)2g
 Bacto Yeast Extract (Difco)2g
 Glucose5g
 Sodium n-hexanoate0.2g
 K solution*10ml
 M solution**2.5ml
 C solution***2.5ml
 Resazurin1mg
 15% Na2CO3 solution20ml
 Cysteine-HCl0.75g
 Distilled water1L
 pH 7 
  Mix ingredients except for Na2CO3 and cysteine-HCl, dispense the medium into suitable culture vessels under a stream of N2/CO2 (80/20), seal with butyl rubber stoppers, and autoclave at 115℃ for 25 min. Aseptically and anaerobically add filter-sterile Na2CO3 and cysteine-HCl solutions, and adjust pH to 7.0 with 3 N HCl.
       
  *K solution
   KH2PO490g
   NaCl90g
   (NH4)2SO490g
   Distilled water1L
       
  **M solution
   MnCl2·4H2O4g
   CoCl2·6H2O4g
   Distilled water1L
       
  ***C solution
   CaCl2·2H2O10.6g
   Distilled water1L
Comment