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Medium Detail Information

Medium No.
995
Medium
Composition
 NH4NO30.1g
 KH2PO40.01g
 Fe(III)-EDTA2.5mg
 Vitamin solution*10ml
 Trace elements solution**10ml
 Methanol***2ml
 Seawater****980ml
 pH 8.1 
       
  *Vitamin solution
   Vitamin B120.275mg
   Biotin0.25mg
   Thiamine-HCl50mg
   Distilled water1L
       
  **Trace elements solution
   2Na-EDTA37.2mg
   CuSO4·5H2O0.025mg
   ZnSO4·7H2O0.575mg
   MnCl2·4H2O0.455mg
   CoCl2·6H2O0.06mg
   (NH4)6Mo7O24·4H2O0.027mg
   Distilled water1L
       
  ***Sterilize separately by filtration.
       
  ****Wako Pure Chemical Ind., Ltd., Osaka, Japan
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