Conca_00080 Conca_00080   Conca_00100 Conca_00100

Conca_00090 : CDS information

close this sectionLocation

Organism
StrainATCC 27449 (=NBRC 13477)
Entry nameConcanamycin A
Contig
Start / Stop / Direction70,836 / 77,006 / + [in whole cluster]
70,836 / 77,006 / + [in contig]
Location70836..77006 [in whole cluster]
70836..77006 [in contig]
TypeCDS
Length6,171 bp (2,056 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)modular polyketide synthase
GeneconF
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • CONF
Reference
ACC
PmId
[16207901] Organization of the biosynthetic gene cluster for the macrolide concanamycin A in Streptomyces neyagawaensis ATCC 27449. (Microbiology. , 2005)
comment
CON6 (2056 aa, approx. 215 kDa) encodes the final module for condensation 14 and has a C-terminal integral thioesterase domain required for lactonization and release of the polyketide chain.

CON5でのmodule数のズレにより、module14=13だと思われる。
methoxymalonyl-ACPを受け入れるAT domainsを同定するためのshort sequence motifについて検討あり。

close this sectionPKS/NRPS Module

13 methoxymalonyl-ACP
KS33..409
AT564..884
DH931..1098
KR1404..1584
ACP1682..1752
TE1833..2045

close this sectionSequence

selected fasta
>polyketide synthase [modular polyketide synthase]
MSTDKVVEALRASLLENERLRGENTRLRDESREPVAIVAMSCRFPGGVRTPEDLWRLLAE
ERDAVSEFPADRGWDAAALYDPDPDTPGTSNTREGGFLHDACGFDAGFFGISPREALAMD
PQQRLLLETCWEAVERGGIDPLSLRGSRTGVYAGVMYNEYGARLQRIPEDVEAFIGTGTV
PSVVTGRIAYILGLEGPAVTIDTACSSSLVALHLACQALRAGEISLALAGGVTVMSTPSL
YVGFSRQRGLAPDGRSKSFAAAADGAGFGEGVGVLLLERLSDARRNGHPVLALVRGSAVN
QDGASNGMTAPNGPSQQRVIQQALAAAGLSPAEVDAVEAHGTGTRLGDPIEAQALLATYG
QGRPADRPLLLGSLKSNLTHTQAAAGVGGVMKMVLAMRHGLLPRTLHIDRPTPQVDWTEG
NIALLTTATPWPDNGHPRRAGVSSFGASGTNAHTVLEAAPADLDTPPDTGESAPAPVAWL
LSGKGEAALRGQAAALHTHLTDDPEARPVDVGLSLASGRSRFADRAVVVGDDRPALLSGL
AALAAGRPDPAVVTARAQPGRKAVFVFPGLGSQWPGMGAELLDTSPEFAARIADCEQALA
PYIDWSVTEVLRGTPGAPTLDRVDVLQPVLFSVMVSLAALWRSYGVEPAAVVGHSQGEVA
AACVAGALTLEDGARVVALRSRALAGLGGGGGMATVVLSQAEVAPYLERRSGKVTVAAVN
GPRVLVVAGPGDEVGALVDELSAEGVMAWRIPVDYASHSDDVSTVRDRLGEDLSSLVPKA
PAVPLVSTVDADWIGPGDLTHEYWYRNLRQTVRFHDAIRLLLDAGHRTFVEVSPHPLLTY
GIGDTAADADADDAVVLGTLRRDEGGLNRMLLALGEAHANGVAVDWRRAFDGTGARLTDL
PTYAFQRTHYWLDVPAHAGDPESAGQTETGHPLLTAAVDLPDGDGVLFTGRLSSRTHPWL
ADHAVSGTVLLPGVAFVEMAAHAAAHVGCTTVEELTLAAPLVLGPPAADEARRLRLTVDA
PDPEGRRAVALYSRPDDPESTEPWVRHAGGTLAGTPASAPGDPGRWPPADGAERLELDSL
YADFEAAGVHYGPLFQGLRAAWRVGDEIHAEVALPESVPGTRESGFAVHPALLDAALQTV
ALRPDSTDPGRTGGVPLPFSWHDVTLAPAADPVLRVVLAPAEQGGDAVSVRITDRTGRPV
VTVGALTLRAAAGAPAAAERSLFRLDWTPLPATDPSPGTWATLGACDGDLLPAGFRADRC
PDLTDVPATSDAVLVLCPPAGTPGPEGARTSVEAVLALLQRWLAEPKFTETPLVLLTRGA
VDLDGAPADLGGAAGLGLLRTAQLEHPGRFVLLDTDGGDGLEAALAHALAEGEPQLALRD
GTLYAPRLVRPPVADRPAGFDPEGTVLVTGGGGTLGGLVARHLVTTHGVRHLLLLGRRGA
AAEGTASLTEELTAAGAEVTVAACDVADREALADCLAAIPSEHPLRAVVHTAGVLDDGLL
EALTPERLSAVLRPKADGAWNLHELTREQDLTAFVLFSSSAGVFGSPGQAAYAAANAFTD
ALAAARHRAGLPGTSLAWGLWEQRTGLTDGLGDTDLRRMARSGVRALPTEEGLALFDAAL
AGDDPVLVPVGLDLRARPGGPVPHLLRSLIRTPAVRRRGPQADASLRPRLAAVPEAEQGE
ILLRLVRTQAAEVLGHADPDALSEGDAFLESGFDSLTAVELRNRLAAALDLRLRPTAVLD
SGTPARLAEHLREELTRNTAPGAGRGVGEAPYDPVSALFRQACEVGRIHEGIQILQQAAK
LRPDFGSGPELLSSSGRRPELLRLHIRDDAPELVCFASVVALGGAHQYSRLASAFEGRYG
VSALDAPGFAPDEPLPADMSSLLDFQTEVLLRRLGDRPKVLLGSSSGGTLAHAAAARLEA
LGAGPAAVVLLDTYLSGDEAMTQFDDVLVSGMFEREDRAAPMDGTRLTAMGRYFQLLDDW
VPPPVEAPVLLVRASSPLAEPAPDSDIDWRASWPSAHSVLDVPGNHWSMMEQHLKTTVDA
VSGWLGTNLPEFSRGI
selected fasta
>polyketide synthase [modular polyketide synthase]
ATGTCCACGGACAAGGTGGTCGAGGCGCTGCGCGCCTCCCTGCTGGAGAACGAGCGGCTG
CGCGGCGAGAACACACGGCTGCGCGACGAGTCCCGGGAACCGGTCGCCATCGTCGCCATG
AGCTGCCGCTTCCCGGGAGGGGTCCGCACCCCGGAGGACCTGTGGCGGCTGCTCGCCGAA
GAACGCGACGCGGTCAGCGAGTTCCCCGCCGACCGGGGCTGGGACGCCGCCGCACTGTAC
GACCCCGACCCCGACACCCCGGGTACGTCCAACACCCGGGAGGGCGGCTTCCTGCACGAC
GCCTGCGGCTTCGACGCCGGCTTCTTCGGGATCTCGCCCCGTGAGGCCCTGGCCATGGAC
CCCCAGCAGCGACTCCTGCTGGAGACCTGCTGGGAGGCCGTCGAGCGCGGTGGCATCGAC
CCGCTGTCCCTGCGCGGCAGCCGCACCGGCGTCTACGCGGGCGTCATGTACAACGAGTAC
GGCGCCCGGCTGCAGCGGATACCGGAGGACGTCGAGGCGTTCATCGGCACCGGCACCGTG
CCCAGCGTGGTCACCGGCCGGATCGCCTACATCCTCGGCCTGGAGGGCCCGGCCGTCACG
ATCGACACCGCGTGCTCGTCGTCGCTGGTCGCCCTGCACCTGGCCTGCCAGGCGCTGCGG
GCGGGGGAGATCTCCCTCGCCCTGGCGGGCGGTGTCACGGTGATGTCCACACCGAGCCTG
TACGTCGGGTTCAGCCGCCAGCGCGGACTGGCGCCGGACGGCCGCAGCAAGTCGTTCGCC
GCGGCCGCCGACGGTGCCGGCTTCGGCGAGGGCGTCGGCGTGCTGCTCCTCGAACGGCTC
TCGGACGCCCGGCGCAACGGCCACCCCGTGCTGGCGCTGGTGCGGGGCTCGGCGGTCAAC
CAGGACGGCGCCAGCAACGGCATGACCGCGCCCAACGGCCCTTCCCAGCAACGGGTGATC
CAGCAGGCCCTGGCGGCGGCCGGCCTCTCACCGGCCGAGGTCGACGCGGTGGAGGCGCAC
GGCACGGGGACCCGCCTCGGCGACCCCATCGAGGCTCAGGCGCTGCTGGCCACCTACGGT
CAGGGCCGCCCGGCGGACCGGCCGCTGCTGCTCGGCTCGCTCAAGTCCAACCTCACCCAC
ACCCAGGCCGCCGCCGGGGTCGGCGGCGTCATGAAGATGGTGCTCGCGATGCGCCACGGC
CTGCTGCCCCGCACCCTGCACATCGACCGTCCCACCCCGCAGGTCGACTGGACCGAGGGC
AACATCGCCCTGCTCACCACGGCCACCCCCTGGCCGGACAACGGCCACCCCCGCCGCGCC
GGCGTCTCCTCCTTCGGCGCCAGCGGCACCAACGCCCACACCGTTCTGGAGGCGGCGCCC
GCCGACCTCGACACACCCCCGGACACCGGGGAATCGGCCCCCGCCCCCGTCGCCTGGCTG
CTCTCGGGCAAGGGCGAAGCGGCGCTGCGCGGCCAGGCCGCCGCCCTCCACACCCACCTC
ACCGACGACCCCGAGGCGCGTCCTGTGGACGTCGGCCTCTCGCTGGCCAGCGGCAGATCC
CGCTTCGCCGACCGTGCCGTCGTCGTCGGCGACGACCGTCCCGCCCTGCTGTCCGGCCTG
GCCGCGCTGGCCGCCGGACGGCCCGACCCGGCGGTCGTCACCGCCCGCGCCCAGCCAGGC
CGCAAGGCCGTCTTCGTCTTCCCCGGCCTCGGCTCCCAGTGGCCCGGCATGGGCGCCGAA
CTCCTCGACACGTCACCGGAGTTCGCCGCCCGGATCGCGGACTGCGAACAGGCGCTCGCC
CCGTACATCGACTGGTCGGTCACCGAGGTGCTCCGTGGCACGCCGGGCGCGCCGACCCTG
GACCGGGTGGACGTCCTCCAGCCCGTCCTGTTCTCCGTGATGGTGTCGCTGGCGGCGCTC
TGGCGGTCGTACGGGGTGGAGCCTGCCGCGGTCGTCGGCCACAGCCAGGGCGAGGTCGCC
GCCGCCTGTGTCGCGGGTGCTCTCACCCTGGAGGACGGGGCCCGCGTGGTGGCGCTGCGC
AGCCGGGCCCTCGCCGGCCTCGGCGGGGGCGGAGGCATGGCCACCGTGGTGCTCTCCCAG
GCCGAGGTCGCCCCCTACCTGGAACGCCGCTCGGGCAAGGTGACGGTCGCCGCCGTCAAC
GGCCCCCGCGTGCTCGTCGTCGCCGGACCCGGCGACGAGGTCGGCGCCCTCGTCGATGAA
CTCTCCGCCGAGGGCGTCATGGCCTGGCGCATCCCGGTGGACTACGCCTCCCACTCGGAC
GACGTGAGCACCGTACGGGACCGGCTGGGCGAGGACCTGTCCTCGCTCGTTCCCAAGGCC
CCGGCCGTGCCCCTGGTGTCCACGGTGGACGCCGACTGGATCGGTCCCGGCGACCTCACC
CACGAGTACTGGTACCGCAACCTCCGCCAGACCGTCCGCTTCCACGACGCGATACGCCTG
CTCCTCGACGCCGGGCACCGCACCTTCGTCGAGGTGAGCCCCCACCCGCTGCTCACCTAC
GGCATCGGCGACACCGCGGCCGACGCCGACGCGGACGACGCCGTGGTGCTGGGCACCCTG
CGGCGCGACGAGGGCGGGCTGAACCGGATGCTGCTGGCGCTCGGCGAGGCGCACGCGAAC
GGCGTGGCCGTCGACTGGCGGCGCGCGTTCGACGGCACCGGGGCCCGTCTGACCGACCTG
CCGACGTACGCCTTCCAGCGCACCCACTACTGGCTCGACGTGCCCGCGCACGCCGGGGAC
CCCGAGTCGGCGGGCCAGACGGAGACGGGGCACCCGCTGCTGACCGCCGCCGTGGATCTG
CCGGACGGCGACGGAGTGCTCTTCACGGGGCGCCTGTCGTCGCGCACCCATCCGTGGCTG
GCGGACCACGCGGTCTCCGGCACGGTGCTGCTGCCCGGCGTCGCCTTCGTGGAGATGGCC
GCCCACGCAGCCGCCCACGTCGGCTGCACCACGGTCGAGGAACTCACCCTGGCCGCACCC
CTCGTGCTGGGGCCCCCAGCCGCCGACGAGGCCCGACGGCTCCGCCTGACCGTGGACGCG
CCCGACCCCGAGGGCCGCCGGGCCGTGGCGCTGTACTCCCGCCCCGACGACCCCGAGTCC
ACCGAACCGTGGGTACGCCACGCGGGGGGCACCCTGGCCGGGACGCCCGCCTCCGCGCCG
GGTGACCCCGGCCGGTGGCCGCCCGCCGACGGCGCCGAGCGGCTCGAACTCGACAGCCTG
TACGCCGACTTCGAGGCGGCCGGCGTCCACTACGGCCCGCTCTTCCAGGGCCTGCGCGCC
GCCTGGCGGGTCGGCGACGAGATCCACGCCGAGGTGGCTCTGCCCGAGTCGGTGCCGGGG
ACCCGCGAGAGCGGCTTCGCCGTCCACCCGGCCCTGCTCGACGCCGCGCTCCAGACAGTC
GCCCTGCGCCCCGACTCCACCGACCCCGGCCGCACGGGAGGCGTGCCGCTGCCCTTCTCG
TGGCACGACGTCACCCTCGCCCCGGCGGCCGACCCGGTCCTGCGGGTCGTGCTGGCCCCG
GCCGAGCAGGGTGGCGACGCCGTGTCGGTACGGATCACCGACCGCACCGGGCGCCCGGTC
GTCACCGTCGGCGCGCTGACCCTGCGGGCGGCGGCGGGTGCTCCTGCCGCCGCCGAGCGG
TCGCTGTTCCGCCTCGACTGGACGCCGCTGCCCGCCACCGATCCGTCCCCCGGCACCTGG
GCCACGCTCGGCGCCTGCGACGGCGACCTGCTGCCGGCCGGGTTCCGGGCCGACCGGTGC
CCCGACCTCACCGATGTGCCCGCCACGTCCGACGCGGTGCTGGTGTTGTGCCCGCCGGCC
GGGACCCCGGGCCCCGAGGGCGCCCGGACCTCCGTCGAAGCGGTCCTCGCCCTGCTCCAA
CGCTGGCTGGCAGAGCCGAAGTTCACCGAAACCCCCCTGGTGCTGCTCACCCGTGGCGCG
GTCGACCTCGACGGCGCCCCCGCGGACCTCGGGGGCGCCGCCGGCCTGGGGCTCCTGCGC
ACCGCCCAGCTGGAGCACCCCGGCCGCTTCGTCCTGCTGGACACCGATGGCGGCGACGGC
CTCGAAGCCGCCCTCGCCCACGCCCTGGCCGAGGGCGAACCCCAACTCGCCCTGCGTGAC
GGCACCTTGTACGCACCCCGCCTGGTCCGCCCGCCCGTCGCCGACCGTCCCGCCGGGTTC
GACCCGGAGGGCACCGTCCTCGTCACCGGAGGCGGAGGCACCCTGGGCGGACTGGTGGCC
CGTCACCTGGTCACCACCCATGGCGTACGGCATCTGCTGCTGCTCGGCCGCCGGGGCGCC
GCCGCCGAAGGCACGGCCTCTCTCACCGAGGAGCTGACCGCGGCGGGAGCCGAGGTCACC
GTCGCGGCGTGCGACGTGGCCGACCGGGAGGCGCTGGCCGACTGCCTCGCGGCGATTCCC
TCGGAGCACCCGCTGCGCGCCGTCGTGCACACGGCCGGCGTCCTGGACGACGGGCTGCTG
GAGGCCCTCACCCCCGAGCGGCTGTCGGCCGTTCTGCGCCCCAAGGCCGACGGCGCCTGG
AACCTGCACGAGCTGACCCGGGAGCAGGATCTGACGGCGTTCGTCCTGTTCTCCTCCTCC
GCCGGTGTCTTCGGCAGCCCCGGCCAGGCCGCCTACGCCGCCGCCAACGCCTTCACCGAC
GCCCTGGCCGCCGCCCGCCACCGGGCGGGGCTGCCCGGCACCTCCCTGGCCTGGGGCCTG
TGGGAGCAGCGCACCGGTCTCACCGACGGCCTCGGCGACACGGACCTGCGGCGGATGGCG
CGGAGCGGGGTGCGCGCCCTGCCCACCGAGGAGGGCCTGGCACTGTTCGACGCGGCACTG
GCGGGCGACGACCCGGTCCTCGTCCCGGTGGGCCTCGATCTGCGGGCCCGGCCCGGTGGC
CCGGTGCCCCACCTGCTGCGGTCGCTGATCCGGACCCCGGCCGTACGCCGGCGTGGCCCG
CAGGCCGACGCGTCGCTGCGGCCCCGGCTGGCCGCCGTGCCGGAGGCCGAACAGGGCGAG
ATCCTGCTGCGGCTGGTGCGGACCCAGGCGGCGGAGGTGCTCGGCCACGCCGACCCGGAC
GCGCTGTCCGAGGGTGACGCCTTCCTGGAGAGCGGGTTCGACTCGCTGACCGCCGTCGAG
TTGCGCAACCGCCTCGCCGCCGCGCTCGACCTGCGGCTGCGCCCCACCGCCGTACTCGAC
TCGGGAACCCCCGCGCGGCTGGCCGAGCACCTGCGGGAGGAACTGACCCGGAACACCGCT
CCCGGAGCCGGGCGGGGGGTCGGTGAAGCACCGTACGACCCGGTCAGCGCCCTGTTCCGG
CAGGCGTGCGAGGTGGGCCGGATCCACGAGGGCATCCAGATCCTCCAGCAGGCCGCGAAG
CTCCGCCCCGACTTCGGGTCCGGCCCCGAGCTGCTGAGCTCGTCCGGACGGCGTCCGGAG
CTGCTGCGGCTGCACATCCGCGACGACGCGCCCGAACTGGTCTGCTTCGCCTCGGTGGTG
GCGCTCGGCGGAGCCCATCAGTACAGCCGTTTGGCGTCCGCCTTCGAGGGCCGCTACGGG
GTCAGCGCGCTGGACGCGCCCGGCTTCGCCCCCGACGAGCCACTGCCCGCCGACATGTCC
TCCCTGCTGGACTTCCAGACCGAGGTCCTCCTGCGGCGCCTCGGTGACCGGCCGAAGGTG
CTGCTCGGCTCCTCGTCGGGCGGCACCCTCGCCCACGCCGCCGCCGCTCGCCTGGAGGCG
CTCGGGGCAGGCCCGGCCGCCGTGGTGCTGCTGGACACGTATCTGTCCGGGGACGAGGCG
ATGACCCAGTTCGACGACGTACTGGTCAGCGGCATGTTCGAGCGCGAGGACCGGGCCGCC
CCGATGGACGGCACCCGGCTGACGGCCATGGGCCGCTACTTCCAGCTCCTGGACGACTGG
GTGCCGCCCCCCGTCGAGGCGCCCGTGCTGCTGGTCCGCGCATCGAGCCCCCTGGCGGAA
CCGGCACCGGACAGCGACATCGACTGGCGGGCGTCATGGCCGTCCGCGCATTCCGTCCTG
GATGTACCAGGAAATCACTGGTCAATGATGGAACAGCATCTGAAAACCACCGTGGACGCC
GTTTCCGGATGGCTCGGAACGAACCTTCCGGAATTCTCCCGGGGTATCTGA
[13] KS33..409
[13] AT564..884
[13] methoxymalonyl-ACP747..759
[13] DH931..1098
[13] KR1404..1584
[13] ACP1682..1752
[13] TE1833..2045
[13] KS97..1227
[13] AT1690..2652
[13] methoxymalonyl-ACP2239..2277
[13] DH2791..3294
[13] KR4210..4752
[13] ACP5044..5256
[13] TE5497..6135

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001031 Thioesterase (Domain)
[1833-2045] 3.8e-30 PF00975
PF00975   Thioesterase
IPR001227 Acyl transferase domain (Domain)
[554-687] 2.59999999999995e-71 G3DSA:3.40.366.10 [755-871] 2.59999999999995e-71 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
[1710-1725] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1686-1751] 4.1e-10 PF00550
PF00550   PP-binding
[1682-1755] 4.5e-17 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1682-1752] PS50075
PS50075   ACP_DOMAIN
[1674-1769] 1.20000117458134e-20 SSF47336
SSF47336   ACP_like
IPR013968 Polyketide synthase, KR (Domain)
[1404-1583] 1.09999999999999e-64 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[33-283] 6.49999999999992e-96 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[291-409] 4.10000000000004e-45 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[564-884] 3.10000000000005e-106 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[562-864] 4.40002866058555e-66 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[689-754] 1.39999892049878e-16 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[32-293] 7.30000000000007e-91 G3DSA:3.40.47.10 [295-461] 2.89999999999999e-62 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[25-407] 8.19994104549873e-100 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1405-1584] 2.89999999999999e-36 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[196-212] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[566-864] 2.2999842048857e-111 SM00827
SM00827   PKS_AT
IPR020802 Polyketide synthase, thioesterase domain (Domain)
[1834-2045] 3.10001307370203e-118 SM00824
SM00824   PKS_TE
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1683-1755] 3.00000067992871e-25 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[931-1098] 6.69996745106047e-77 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[35-461] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1404-1584] 1.70000295590053e-62 SM00822
SM00822   PKS_KR
SignalP
[1-3] 0.093 Signal
Eukaryota   
TMHMM No significant hit
Conca_00080 Conca_00080   Conca_00100 Conca_00100
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