Conca_00090 Conca_00090   Conca_00110 Conca_00110

Conca_00100 : CDS information

close this sectionLocation

Organism
StrainATCC 27449 (=NBRC 13477)
Entry nameConcanamycin A
Contig
Start / Stop / Direction77,034 / 77,906 / + [in whole cluster]
77,034 / 77,906 / + [in contig]
Location77034..77906 [in whole cluster]
77034..77906 [in contig]
TypeCDS
Length873 bp (290 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative oxidoreductase
Product (GenBank)methoxymalonate biosynthesis protein
Gene
Gene (GenBank)
EC number
Keyword
  • methoxymalonyl-ACP
Note
Note (GenBank)
  • ORF1*
Reference
ACC
PmId
[16207901] Organization of the biosynthetic gene cluster for the macrolide concanamycin A in Streptomyces neyagawaensis ATCC 27449. (Microbiology. , 2005)
comment
ORF1* methoxymalonate biosynthesis (putative dehydrogenase)

ORF1*はmethoxymalonyl-ACPの生合成に関与が示唆されている遺伝子を含んだascomycin biosynthetic clusterにあるFkbB(←FkbKの間違いと思われる。FkbKは9th, 66%, 2e-90)にhomologousである。
Related Reference
ACC
Q8KUG1
NITE
Ansam_00150
PmId
[11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002)
comment
15th(Q8KUG1) 60%, 5e-76
Actinosynnema pretiosum subsp. auranticum_asm13
3-hydroxyacyl-CoA dehydrogenase

S. lividansで、AT domainsのひとつがmethoxymalonateに特異的なATで置換されているerythromycin PKSのカセットとasm13-17を同時発現すると、予測された位置にmethoxymalonateが取り込まれた6- deoxyerythronolide Bの派生体が産生された。よって、asm13-17はmethoxymalonyl-ACP生合成に十分である。
ACC
Q7BQ74
PmId
[16983083] Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. (Proc Natl Acad Sci U S A. , 2006)
comment
32nd(Q7BQ74) 39%, 2e-52
Bacillus cereus_zmaG
ZmaG

ZmaGは、珍しいPKS伸長units, hydroxymalonyl-ACP and aminomalonyl-ACPの生合成に共通して関連する。

ZmaE or ZmaIと共に働く。
ZmaI: eryl-ZmaH(ACP)→aminomalonyl-ZmaH(ACP)
ZmaE: glyceryl-ZmaD(ACP)→hydroxymalonyl-ZmaD(ACP)
ACC
P52041
PmId
[8655474] Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. (J Bacteriol. , 1996)
comment
171st(P52041) 40%, 7e-48
Clostridium acetobutylicum_hbd
3-hydroxybutyryl-CoA dehydrogenase

BCS (butyryl-CoA synthesis) operon (crt-bcd-etfB-etfA-hbd)のクローニングとシークエンス
crt geneの上流にあるプロモーターによって制御されるtranscriptional unitであることを確認

BCS operonをE. coliで大量発現。crotonase, beta-hydroxybutyryl-CoA dehydrogenase activity確認

BCS operonをC. acetobutylicum で発現。crotonase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase activity上昇
ACC
Q9ADL9
NITE
Soraph_00040
PmId
[12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002)
[15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004)
comment
Blast 218th, id42%, 3e-47
Sorangium cellulosum_sorD
Beta-hydroxybutyryl-CoA dehydrogenase

---
[PMID: 12039053](2002)
Soraphen A biosynthetic gene clusterの報告。
SorD(295aa): beta-Hydroxybutyryl-CoA dehydrogenase

Module 3,7で取り込まれるMethoxymalonyl-CoAを合成する。
本ORFはCH2OHをCHOにする。

---
[PMID: 15289572](2004)
sorC破壊株はsoraphen A非産生。

S. lividansでのsor genes異種性発現。
SorABRCDFE株 + cinnamate
SorABRCDFE株 + badA(benzoate-CoA ligase) + benzoate or cinnamate
の組み合わせでSoraphen A産生。
SorABRCDFE株で発現されているのは、the sorA, and the sorB4M, sorRCDFE operons.

close this sectionSequence

selected fasta
>putative oxidoreductase [methoxymalonate biosynthesis protein]
MSENNAGDAPNAHRLTILGAGVMGVGIATLAVGHGVPVALIDVDQAKRAEAEARIAHELR
LAGLMGALPDDVPTGELVISDSLSDAAGSTAVIEAVTEISELKEKVIAEVSVIVSPGTLL
VSNTSSIPIDELASAAARPAELVGTHFMNPSYLIRTVEVIRGARTGEAAMDSVRALLTTL
RRRPVVVRDAPGFVTSRVLHPMINDAARVVQEGTATAEDVDALMQGCLGHATGPLRTADL
IGIDNLVDSLNVLYERTGDSGCRPCDLLLEKVRAGDIGRKSGRGFYEYEG
selected fasta
>putative oxidoreductase [methoxymalonate biosynthesis protein]
ATGTCGGAAAACAACGCGGGTGACGCTCCGAACGCACATCGCCTCACCATCCTGGGCGCC
GGCGTCATGGGCGTGGGCATCGCCACCCTGGCGGTCGGCCACGGCGTACCGGTGGCCCTC
ATCGACGTCGACCAGGCGAAACGCGCGGAGGCGGAGGCCAGGATCGCCCACGAACTGCGG
CTCGCGGGGCTGATGGGCGCGCTGCCGGACGACGTCCCCACCGGGGAACTCGTCATCAGT
GATTCCTTGTCCGACGCGGCCGGTTCCACCGCGGTCATCGAGGCCGTGACAGAAATATCG
GAGCTCAAGGAGAAAGTGATCGCGGAGGTTTCCGTCATCGTTTCACCTGGTACTCTCCTG
GTGTCGAACACGTCGTCCATTCCCATTGACGAATTGGCCTCGGCGGCCGCGCGTCCGGCG
GAACTCGTCGGCACGCATTTCATGAACCCGTCCTATCTGATCCGCACGGTCGAGGTCATC
CGCGGCGCCCGCACCGGCGAGGCCGCCATGGACTCCGTACGGGCCCTGCTCACCACCCTG
CGCCGTCGGCCCGTCGTGGTCCGGGACGCCCCCGGCTTCGTGACCAGCCGGGTCCTGCAC
CCGATGATCAACGACGCGGCCCGGGTGGTACAGGAGGGAACCGCGACCGCCGAGGACGTC
GACGCCCTCATGCAGGGCTGCCTGGGGCACGCCACAGGTCCGTTGCGCACCGCGGACCTC
ATCGGCATCGACAACCTCGTCGACTCGCTGAACGTGCTGTACGAGCGCACCGGTGACAGT
GGCTGTCGTCCGTGCGACCTGCTGCTGGAGAAGGTGCGGGCAGGCGACATCGGCCGGAAG
TCCGGCCGTGGTTTCTACGAATACGAGGGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain)
[192-288] 7.20000000000003e-29 PF00725
PF00725   3HCDH
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain)
[15-189] 3.89999999999997e-46 PF02737
PF02737   3HCDH_N
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain)
[191-290] 2.800005042611e-29 SSF48179
SSF48179   6DGDH_C_like
IPR013328 Dehydrogenase, multihelical (Domain)
[194-289] 3.39999999999999e-28 G3DSA:1.10.1040.10
G3DSA:1.10.1040.10   Opine_DH
IPR016040 NAD(P)-binding domain (Domain)
[10-193] 3.09999999999998e-42 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR022694 3-hydroxyacyl-CoA dehydrogenase (Family)
[1-289] 5.40001756046195e-59 PIRSF000105
PIRSF000105   HCDH
SignalP
[1-1] Signal-anchor
Eukaryota   
TMHMM
[15-37] Transmembrane (o-i)
Transmembrane 1   
Conca_00090 Conca_00090   Conca_00110 Conca_00110
Page top