Borre_00040 Borre_00040   Borre_00060 Borre_00060

Borre_00050 : CDS information

close this sectionLocation

Organism
StrainTü4055
Entry nameBorrelidin
Contig
Start / Stop / Direction5,123 / 5,953 / + [in whole cluster]
5,123 / 5,953 / + [in contig]
Location5123..5953 [in whole cluster]
5123..5953 [in contig]
TypeCDS
Length831 bp (276 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative non-heme haloperoxidase
Product (GenBank)hypothetical protein
Gene
Gene (GenBank)
EC number1.11.1.-
Keyword
Note
Note (GenBank)
  • ORFB5
Reference
ACC
PmId
[15112998] Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: cluster analysis and assignment of functions. (Chem Biol. , 2004)
comment
Borrelidin生合成gene clusterのクローニング。
orfB5: closest similar to nonheme chloroperoxidase

borrelidin非産生であるS.coelicolorのタンパクに明らかな類似があるが、遺伝子配置は異なるもの。
Related Reference
ACC
P49323
PmId
[8157602] Chloroperoxidase from Streptomyces lividans: isolation and characterization of the enzyme and the corresponding gene. (J Bacteriol. , 1994)
[9642069] Structural investigation of the cofactor-free chloroperoxidases. (J Mol Biol. , 1998)
comment
3rd, 89%, 1e-142
Streptomyces lividans_cpo
Non-heme chloroperoxidase(EC 1.11.1.10)

TK64_chloroperoxidase cpo(EC1.11.1.10).
non-metal, homodimer。構造解析済。
活性はchloridationだが、いくつかの基質(indole等)に対してはbrominate可能。

close this sectionSequence

selected fasta
>putative non-heme haloperoxidase [hypothetical protein]
MGTVTTSDGTSIFYKDWGPRDAPPIVFHHGWPLTADDWDNQMLFFLSHGYRVIAHDRRGH
GRSGQPSTGHEMDTYAADVAALTEALDLRDAVHIGHSTGGGEVARYVARAEPGRVAKAVL
VGAVPPVMVKSDANPGGTPIEVFDGFRTALAANRAQFYIDVPSGPFYGFNREGAKVSQGL
IDNWWRQGMSGAANAHYECIKAFSETDFTEDLKAIDVPVLVAHGTDDQVVPYADSAPLSV
KLLKNGTLKSYEGLPHGMLSTHPEVVNPDLLDFVRS
selected fasta
>putative non-heme haloperoxidase [hypothetical protein]
ATGGGTACGGTCACCACCTCCGACGGCACGAGCATCTTCTACAAGGACTGGGGCCCGCGC
GACGCCCCGCCGATCGTCTTCCACCACGGCTGGCCGCTCACCGCGGACGACTGGGACAAC
CAGATGCTGTTCTTCCTCTCGCACGGCTACCGTGTGATCGCCCACGACCGGCGCGGCCAC
GGCCGCTCGGGCCAGCCCTCGACGGGCCACGAGATGGACACCTACGCCGCCGACGTCGCG
GCGCTGACCGAAGCGCTCGACCTGCGGGACGCCGTCCACATCGGGCATTCGACCGGCGGC
GGCGAGGTCGCGCGCTATGTGGCGCGCGCCGAACCGGGCCGGGTCGCCAAGGCCGTGCTG
GTCGGCGCCGTGCCGCCGGTGATGGTCAAGTCCGACGCCAACCCCGGCGGCACCCCGATC
GAGGTCTTCGACGGGTTCCGCACGGCCCTGGCCGCCAACCGGGCCCAGTTCTACATCGAC
GTGCCCTCCGGCCCCTTCTACGGATTCAACCGGGAGGGCGCGAAGGTCTCCCAGGGCCTG
ATCGACAACTGGTGGCGGCAGGGCATGTCGGGCGCGGCCAACGCCCACTACGAGTGCATC
AAGGCGTTCTCCGAGACCGACTTCACCGAGGACCTCAAGGCCATCGACGTGCCGGTGCTG
GTCGCGCACGGCACCGACGACCAGGTCGTGCCCTACGCGGACTCGGCGCCGCTGTCGGTG
AAGCTCCTGAAGAACGGCACCCTCAAGTCGTACGAAGGGCTCCCGCACGGCATGCTCTCC
ACCCACCCCGAGGTGGTCAACCCCGACCTCCTGGACTTCGTGAGGTCCTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000073 Alpha/beta hydrolase fold-1 (Domain)
[49-64] 1e-08 PR00111 [93-106] 1e-08 PR00111 [219-233] 1e-08 PR00111
PR00111   ABHYDROLASE
IPR000639 Epoxide hydrolase-like (Family)
[29-47] 6.20000124972815e-06 PR00412 [49-64] 6.20000124972815e-06 PR00412 [251-273] 6.20000124972815e-06 PR00412
PR00412   EPOXHYDRLASE
SignalP
[1-35] 0.193 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Borre_00040 Borre_00040   Borre_00060 Borre_00060
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