Borre_00320 Borre_00320   Borre_00340 Borre_00340

Borre_00330 : CDS information

close this sectionLocation

Organism
StrainTü4055
Entry nameBorrelidin
Contig
Start / Stop / Direction62,885 / 62,586 / - [in whole cluster]
62,885 / 62,586 / - [in contig]
Locationcomplement(62586..62885) [in whole cluster]
complement(62586..62885) [in contig]
TypeCDS
Length300 bp (99 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.2 function unknown
Productputative acetyltransferase(fragment)
Product (GenBank)hypothetical protein
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORFB13b
Reference
ACC
PmId
[15112998] Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: cluster analysis and assignment of functions. (Chem Biol. , 2004)
comment
Borrelidin生合成gene clusterのクローニング。
orfB13: closest similar to putative acetyltransferase

borrelidin生合成に必要な機能や役割をもつタンパクに類似性がない、またはputative functionsがborrelidin生合成のために必要かわからないもの。

---
論文では163aaのorfB13であるが、UniProtでは2つのfragmentsに分かれている。
nt.position 62885でのG deletionによるframeshift.
N-term> Q70HY3: orfB13a(63aa)
C-term> Q70HY4: orfB13b(99aa)
Family/Domain
FD
IPR000182
comment
[IPR000182] GCN5-related N-acetyltransferase (Domain)
function; N-acetyltransferase activity

close this sectionSequence

selected fasta
>putative acetyltransferase(fragment) [hypothetical protein]
VDAEDATDRVLGFYALLLAPAELDLLFVQDGTQGRGIGRLLVDHMKRRARAAGLDRVRVV
SHPPAEGFYRAVGALPTGTARANPPAVAWDRPVLEFLIP
selected fasta
>putative acetyltransferase(fragment) [hypothetical protein]
GTCGACGCCGAGGACGCCACGGACCGGGTGCTCGGCTTCTACGCGCTGCTCCTCGCGCCG
GCGGAGCTGGACCTGCTGTTCGTCCAGGACGGTACCCAGGGCCGCGGCATCGGACGGCTG
CTTGTCGATCACATGAAGAGGCGGGCCCGCGCGGCAGGGCTGGACCGTGTCCGGGTGGTG
TCGCACCCTCCGGCCGAGGGCTTCTACCGCGCTGTGGGCGCCCTGCCCACCGGAACCGCC
CGCGCGAACCCGCCCGCGGTGGCCTGGGACCGACCCGTGCTGGAATTCCTGATCCCGTAA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000182 GNAT domain (Domain)
[1-94] PS51186
PS51186   GNAT
[6-73] 3.2e-09 PF13673
PF13673   Acetyltransf_10
IPR016181 Acyl-CoA N-acyltransferase (Domain)
[3-98] 2.09999806691534e-14 SSF55729
SSF55729   Acyl_CoA_acyltransferase
[4-80] 2.1e-14 G3DSA:3.40.630.30
G3DSA:3.40.630.30   Acyl_CoA_acyltransferase
SignalP
[1-23] 0.298 Signal
Eukaryota   
TMHMM No significant hit
Borre_00320 Borre_00320   Borre_00340 Borre_00340
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