Crypto_00060 Crypto_00060   Crypto_00080 Crypto_00080

Crypto_00070 : CDS information

close this sectionLocation

Organism
StrainATCC 53789
Entry nameCryptophycin 1
Contig
Start / Stop / Direction47,812 / 48,153 / + [in whole cluster]
47,812 / 48,153 / + [in contig]
Location47812..48153 [in whole cluster]
47812..48153 [in contig]
TypeCDS
Length342 bp (113 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Product3-methylaspartate 1-decarboxylase
Product (GenBank)CrpG
Gene
Gene (GenBank)crpG
EC number4.1.1.-
Keyword
  • alpha-methyl-beta-alanine
Note
Note (GenBank)
  • decarboxylase
Reference
ACC
PmId
[17240975] Biosynthetic characterization and chemoenzymatic assembly of the cryptophycins. Potent anticancer agents from cyanobionts. (ACS Chem Biol. , 2006)
[17240971] Cryptophycin anticancer drugs revisited. (ACS Chem Biol. , 2006)
[17600793] Characterization of the beta-methylaspartate-alpha-decarboxylase (CrpG) from the cryptophycin biosynthetic pathway. (Chembiochem. , 2007)
comment
[PMID: 17240975](2006)
comment in: [PMID: 17240971](2006)

cryptophycin生合成遺伝子を同定した論文。
Supplementary Table 1.
CrpG: amino acid decarboxylase

ラベリング実験などでmethyl beta-alanineがmethyl-aspartateから形成されるかどうかを検討。
crptophycin1のunitCであるmethyl-beta-alanineの2R-stereoisomerを産出するため、CrpGはR-methyl aspartateに対して高い選択性を示す。

---
[PMID:17600793](2007) abstract
上記cluster報告論文と同じ著者ら。

CrpGをクローニング、過剰発現、精製、活性確認。
(2S,3R)-3-methylaspartic acidをdecarboxylateして、3-amino-2(R)-methylpropionic acidを形成するbeta-methylaspartate-alpha-decarboxylaseである。
3-amino-2(R)-methylpropionic acidはcryptophycinsのUnit Cに取り込まれる。

close this sectionSequence

selected fasta
>3-methylaspartate 1-decarboxylase [CrpG]
MRIMLKSKIHRATVTEANVNYIGSITVDKVLMEKADILPGEKVMVVDNTNGNRLETYVLE
GEENSGVICMNGGSAHLVNSGDLITLLAFEVTDEIKEPKKIIVDENNKFLKYL
selected fasta
>3-methylaspartate 1-decarboxylase [CrpG]
ATGCGTATTATGTTGAAGTCGAAAATTCACAGAGCGACGGTGACGGAAGCCAACGTTAAC
TACATCGGAAGTATTACAGTAGACAAAGTTCTGATGGAAAAGGCAGACATACTACCGGGT
GAAAAGGTTATGGTGGTGGACAACACTAATGGTAATCGTCTAGAAACCTATGTCCTAGAA
GGTGAGGAAAATTCCGGGGTAATCTGTATGAACGGTGGCTCCGCCCACCTAGTCAATTCA
GGAGACCTTATCACATTGCTAGCATTCGAGGTAACTGACGAAATCAAGGAACCGAAAAAA
ATTATCGTGGATGAAAACAACAAGTTTCTCAAGTACCTGTAA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003190 Aspartate decarboxylase (Family)
[1-109] 3.59998852907783e-45 PIRSF006246
PIRSF006246   Asp_decarbox
[4-89] PD009294
PD009294   Asp_decarbox
[2-111] 2.59999999999998e-36 TIGR00223
TIGR00223   PanD
[2-109] 1.2e-41 PF02261
PF02261   Asp_decarbox
IPR009010 Aspartate decarboxylase-like fold (Domain)
[1-113] 2.80000504261099e-37 SSF50692
SSF50692   Asp_decarb_fold
[2-111] 5.49999999999995e-43 G3DSA:2.40.40.20
G3DSA:2.40.40.20   Asp_decarboxylase-like_fold
SignalP No significant hit
TMHMM No significant hit
Crypto_00060 Crypto_00060   Crypto_00080 Crypto_00080
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