Megalo_00060 Megalo_00060   Megalo_00080 Megalo_00080

Megalo_00070 : CDS information

close this sectionLocation

Organism
StrainNRRL 3275 (=NBRC 14744)
Entry nameMegalomicin
Contig
Start / Stop / Direction6,592 / 7,197 / + [in whole cluster]
6,592 / 7,197 / + [in contig]
Location6592..7197 [in whole cluster]
6592..7197 [in contig]
TypeCDS
Length606 bp (201 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose epimerase
Product (GenBank)TDP-4-keto-6-deoxyhexose 3,5-epimerase
Gene
Gene (GenBank)megDIV
EC number5.1.3.-
Keyword
  • megosamine
  • mycarose
Note
Note (GenBank)
Reference
ACC
PmId
[10972798] Biosynthesis of the anti-parasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea. (Mol Microbiol. , 2000)
[20418422] TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli. (Appl Environ Microbiol. , 2010)
comment
[PMID: 10972798](2000)
megalomicin (meg) biosynthetic gene clusterの報告。
megDIV: 3,5-epimerase

megDIVはdTDP-L-megosamine生成に関与すると提唱。
TDP-L-megosamineの前駆体であるdTDP-L-daunosamineの生成について述べられている[PMID: 10631513]を参照して割り当て。
dnmT, dnmU, dnmJ and dnmVがmeg clusterのmegT, megDIV, megDII and megDVに相当。

-----
[PMID: 20418422](2010)

異なる遺伝子セットを含んだオペロンによってE.coliで産生されたdTDP-sugar中間体のLC/MS/MS analysis。
D and L epimersの解析結果が同じであるとされ、はっきり証明できているとは言いがたい。
Related Reference
ACC
Q9S0P2
NITE
Aver_00150
PmId
[11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001)
comment
Blast 12th, id56%, 6e-53
Streptomyces avermitilis_aveBV(avrF)
dTDP-4-keto-6-deoxyhexose 3,5-epimerase
[Aver_00150]dTDP-4-keto-6-deoxyhexose 5-epimerase

Table1.
avrF/aveBV: dTDP-4-keto-6-deoxyglucose 3-epimerase　
(本文を読むと5-epimeraseの間違いと思われる。)

S.lividansでS.avermitilis avrBCDEFGHIを発現させて外因性aglyconeと反応させて産物解析。
delta-avrFな発現株では、C-13にD-olivoseが1つbeta-glycoside結合したavermectin A1a3を産生する。
avermectin A1a3を産生できるのはdelta-avrFのみ。
C-3での立体化学は最終的に3-O-methyltransferaseによって決定されるので、AvrF proteinは5-epimeraseであって3,5-epimeraseではない。

close this sectionSequence

selected fasta
>putative NDP-hexose epimerase [TDP-4-keto-6-deoxyhexose 3,5-epimerase]
MRVEELGIEGVFTFTPQTFADERGVFGTAYQEDVFVAALGRPLFPVAQVSTTRSRRGVVR
GVHFTTMPGSMAKYVYCARGRAMDFAVDIRPGSPTFGRAEPVELSAESMVGLYLPVGMGH
LFVSLEDDTTLVYLMSAGYVPDKERAVHPLDPELALPIPADLDLVMSERDRVAPTLREAR
DQGILPDYAACRAAAHRVVRT
selected fasta
>putative NDP-hexose epimerase [TDP-4-keto-6-deoxyhexose 3,5-epimerase]
GTGAGGGTCGAGGAGCTGGGCATCGAGGGGGTCTTCACCTTCACCCCGCAGACGTTCGCC
GACGAGCGGGGGGTGTTCGGCACGGCGTACCAGGAGGACGTGTTCGTGGCGGCGCTCGGC
CGCCCGCTGTTCCCGGTGGCCCAGGTCAGCACCACCCGGTCCCGGCGGGGTGTGGTCCGG
GGGGTGCACTTCACGACGATGCCCGGCTCCATGGCGAAGTACGTCTACTGCGCCAGGGGT
AGGGCGATGGACTTCGCCGTCGACATCCGGCCCGGTTCCCCGACCTTCGGCCGGGCCGAG
CCGGTCGAGCTCTCCGCCGAGTCGATGGTCGGGCTGTACCTTCCCGTGGGCATGGGCCAC
CTGTTCGTCTCCCTGGAGGACGACACCACCCTCGTCTACCTGATGTCCGCCGGTTACGTC
CCCGACAAGGAACGGGCGGTGCACCCCCTGGATCCGGAGCTGGCGTTGCCGATCCCGGCC
GACCTCGACCTCGTCATGTCCGAGCGGGACCGGGTCGCACCCACCCTCCGGGAGGCCCGG
GACCAGGGGATCCTGCCCGACTACGCCGCCTGCCGGGCCGCCGCGCACCGGGTGGTGCGG
ACGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000888 dTDP-4-dehydrorhamnose 3,5-epimerase-related (Family)
[1-183] PD001462
PD001462   dTDP_sugar_isom
[4-178] 9.29999999999998e-50 PF00908
PF00908   dTDP_sugar_isom
IPR011051 RmlC-like cupin domain (Domain)
[1-183] 4.39999001237725e-63 SSF51182
SSF51182   RmlC_like_cupin
IPR014710 RmlC-like jelly roll fold (Domain)
[1-191] 4.9e-56 G3DSA:2.60.120.10
G3DSA:2.60.120.10   RmlC-like_jellyroll
SignalP
[1-20] 0.119 Signal
Bacteria, Gram-negative   
[1-20] 0.075 Signal
Eukaryota   
TMHMM No significant hit
Megalo_00060 Megalo_00060   Megalo_00080 Megalo_00080
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