Megalo_00070 Megalo_00070   Megalo_00090 Megalo_00090

Megalo_00080 : CDS information

close this sectionLocation

Organism
StrainNRRL 3275 (=NBRC 14744)
Entry nameMegalomicin
Contig
Start / Stop / Direction7,220 / 8,206 / + [in whole cluster]
7,220 / 8,206 / + [in contig]
Location7220..8206 [in whole cluster]
7220..8206 [in contig]
TypeCDS
Length987 bp (328 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductdTDP-hexose C-4 ketoreductase
Product (GenBank)TDP-4-keto-6-deoxyhexose 4-ketoreductase
Gene
Gene (GenBank)megDV
EC number
Keyword
  • L-megosamine
Note
Note (GenBank)
Reference
ACC
PmId
[10972798] Biosynthesis of the anti-parasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea. (Mol Microbiol. , 2000)
[20418422] TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli. (Appl Environ Microbiol. , 2010)
comment
[PMID: 10972798](2000)
megalomicin (meg) biosynthetic gene clusterの報告。
megDV: : 4-ketoreductase

megDVはdTDP-L-megosamine生成に関与すると提唱。
TDP-L-megosamineの前駆体であるdTDP-L-daunosamineの生成について述べられている[PMID: 10631513]を参照して割り当て。
dnmT, dnmU, dnmJ and dnmVがmeg clusterのmegT, megDIV, megDII and megDVに相当。

---
[PMID: 20418422](2010)

異なる遺伝子セットを含んだオペロンによってE.coliで産生されたdTDP-sugar中間体のLC/MS/MS analysis。
MegDVがmegosamine生合成に特異的に関与する4-ketoreductaseであることを確認。

close this sectionSequence

selected fasta
>dTDP-hexose C-4 ketoreductase [TDP-4-keto-6-deoxyhexose 4-ketoreductase]
MVVLGASGFLGSAVTHALADLPVRVRLVARREVVVPSGAVADYETHRVDLTEPGALAEVV
ADARAVFPFAAQIRGTSGWRISEDDVVAERTNVGLVRDLIAVLSRSPHAPVVVFPGSNTQ
VGRVTAGRVIDGSEQDHPEGVYDRQKHTGEQLLKEATAAGAIRATSLRLPPVFGVPAAGT
ADDRGVVSTMIRRALTGQPLTMWHDGTVRRELLYVTDAARAFVTALDHADALAGRHFLLG
TGRSWPLGEVFQAVSRSVARHTGEDPVPVVSVPPPAHMDPSDLRSVEVDPARFTAVTGWR
ATVTMAEAVDRTVAALAPRRAAAPSEPS
selected fasta
>dTDP-hexose C-4 ketoreductase [TDP-4-keto-6-deoxyhexose 4-ketoreductase]
GTGGTGGTGCTCGGCGCGTCGGGTTTCCTGGGTTCGGCGGTCACCCACGCCCTGGCCGAC
CTCCCGGTGCGGGTGCGGCTCGTCGCCCGGCGGGAGGTCGTCGTGCCCTCCGGTGCCGTC
GCCGACTACGAGACGCACCGGGTGGACCTCACCGAACCCGGAGCGCTCGCGGAGGTGGTC
GCGGACGCCCGGGCGGTCTTCCCGTTCGCCGCCCAGATCAGGGGTACGTCAGGGTGGCGG
ATCAGCGAGGACGACGTGGTCGCCGAACGGACGAACGTCGGCCTGGTCCGGGACCTGATC
GCCGTCCTGTCCCGCTCGCCGCACGCCCCGGTGGTGGTCTTCCCGGGCAGCAACACGCAG
GTCGGCAGGGTCACCGCCGGCCGGGTCATCGACGGCAGCGAGCAGGACCACCCCGAGGGC
GTCTACGACAGGCAGAAACACACCGGGGAACAGCTGCTCAAGGAGGCCACTGCGGCCGGG
GCGATCCGGGCGACCAGTCTGCGGCTGCCCCCGGTGTTCGGGGTGCCCGCCGCCGGCACC
GCCGACGACCGGGGGGTGGTCTCCACCATGATCCGTCGGGCCCTGACCGGCCAACCGCTG
ACGATGTGGCACGACGGCACCGTCCGGCGTGAACTGCTGTACGTGACCGACGCCGCCCGG
GCCTTCGTCACCGCCCTGGACCACGCCGACGCGCTCGCCGGACGCCACTTCCTGTTGGGG
ACGGGGCGTTCCTGGCCGCTGGGCGAGGTCTTCCAGGCGGTCTCGCGCAGCGTCGCCCGG
CACACCGGCGAGGACCCGGTGCCGGTGGTCTCGGTGCCGCCTCCGGCGCACATGGACCCG
TCGGACCTGCGCAGCGTGGAGGTCGACCCCGCCCGGTTCACGGCTGTCACCGGGTGGCGG
GCCACGGTCACGATGGCGGAGGCGGTCGACCGGACGGTGGCGGCGTTGGCCCCCCGCCGG
GCCGCCGCCCCGTCCGAGCCCTCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[1-240] 5.10000000000004e-46 PF01370
PF01370   Epimerase
IPR016040 NAD(P)-binding domain (Domain)
[2-194] 1.99999999999999e-24 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-41] 0.297 Signal
Bacteria, Gram-positive   
[1-19] 0.986 Signal
Eukaryota   
[1-19] 0.9 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Megalo_00070 Megalo_00070   Megalo_00090 Megalo_00090
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