Megalo_00100 : CDS information

Location

Organism	Micromonospora megalomicea subsp. nigra
Strain	NRRL 3275 (=NBRC 14744)
Entry name	Megalomicin
Contig	AF263245
Start / Stop / Direction	10,479 / 9,226 / - [in whole cluster] 10,479 / 9,226 / - [in contig]
Location	complement(9226..10479) [in whole cluster] complement(9226..10479) [in contig]
Type	CDS
Length	1,254 bp (417 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	dTDP-L-mycarose glycosyltransferase
Product (GenBank)	TDP-mycarose glycosyltransferase
Gene
Gene (GenBank)	megBV
EC number
Keyword	mycarose
Note
Note (GenBank)
Reference	ACC Q9F832 PmId [10972798] Biosynthesis of the anti-parasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea. (Mol Microbiol. , 2000) [15870344] Production of the potent antibacterial polyketide erythromycin C in Escherichia coli. (Appl Environ Microbiol. , 2005) comment [PMID: 10972798](2000) megalomicin (meg) biosynthetic gene clusterの報告。 megBV: glycosyltransferase --- [PMID: 15870344](2005) G-1-P → dTDP-L-mycaroseの変換に必要な7つの遺伝子(megDIV, BII, M, L, BVI, BIV, BIII)とmycarosyltransferase_megBV、C-6 hydroxylase_megFを含んだmycarose operon(pKOS452-53e)を構築し、E.coliで発現。LC/MS解析により、6-deoxyerythronolide Bから3-mycarosyl-erythronolide Bが得られることを確認。

Sequence

>dTDP-L-mycarose glycosyltransferase [TDP-mycarose glycosyltransferase]
MRVLLTSFAHRTHFQGLVPLAWALHTAGHDVRVASQPELTDVVVGAGLTSVPLGSDHRLF
DISPEAAAQVHRYTTDLDFARRGPELRSWEFLHGIEEATSRFVFPVVNNDSFVDELVEFA
MDWRPDLVLWEPFTFAGAVAAKACGAAHARLLWGSDLTGYFRSRSQDLRGQRPADDRPDP
LGGWLTEVAGRFGLDYSEDLAVGQWSVDQLPESFRLETGLESVHTRTLPYNGSSVVPQWL
RTSDGVRRVCFTGGYSALGITSNPQEFLRTLATLARFDGEIVVTRSGLDPASVPDNVRLV
DFVPMNILLPGCAAVIHHGGAGSWATALHHGVPQISVAHEWDCVLRGQRTAELGAGVFLR
PDEVDADTLWQALATVVEDRSHAENAEKLRQEALAAPTPAEVVPVLEALAHQHRADR

>dTDP-L-mycarose glycosyltransferase [TDP-mycarose glycosyltransferase]
GTGCGGGTCCTGCTCACCTCGTTCGCGCACCGTACCCACTTCCAGGGCCTCGTCCCCCTG
GCCTGGGCGCTGCACACCGCCGGCCACGACGTCCGGGTGGCCAGTCAGCCCGAGCTCACC
GACGTCGTCGTCGGCGCGGGACTGACCTCGGTGCCACTCGGCTCGGACCACCGACTCTTC
GACATCTCCCCTGAGGCCGCCGCGCAGGTGCACCGCTACACCACCGACCTGGACTTCGCC
CGACGCGGACCGGAACTGCGCTCGTGGGAGTTCCTGCACGGCATCGAGGAGGCCACCTCC
CGGTTCGTCTTCCCGGTGGTCAACAACGACTCCTTCGTCGACGAACTCGTCGAGTTCGCC
ATGGACTGGCGTCCCGACCTGGTGCTCTGGGAGCCCTTCACCTTCGCCGGGGCGGTCGCG
GCCAAGGCCTGCGGGGCCGCCCACGCCCGGCTGCTGTGGGGCAGCGACCTCACCGGCTAC
TTCCGCAGCCGCTCCCAGGACCTGCGCGGGCAGCGTCCCGCCGACGACCGGCCGGACCCG
CTCGGCGGCTGGCTCACCGAGGTCGCCGGCCGGTTCGGCCTCGACTACAGCGAGGACCTC
GCTGTCGGACAGTGGAGCGTCGACCAGCTGCCCGAGAGCTTCCGCCTGGAGACCGGACTG
GAGTCGGTGCACACCCGGACTTTGCCCTACAACGGGTCCTCCGTCGTGCCGCAGTGGCTC
AGGACCTCGGACGGAGTCCGCCGGGTCTGCTTCACCGGGGGATACTCGGCGCTGGGCATC
ACCTCGAACCCGCAGGAGTTCCTCCGGACACTGGCCACCCTCGCGCGGTTCGACGGCGAG
ATCGTGGTGACCCGCTCCGGGCTCGATCCGGCGTCGGTGCCGGACAACGTCCGCCTGGTG
GACTTCGTTCCCATGAACATCCTGCTGCCGGGCTGTGCCGCCGTCATCCACCACGGCGGT
GCCGGAAGCTGGGCCACCGCGCTGCACCACGGCGTACCGCAGATCTCCGTCGCCCACGAG
TGGGACTGTGTCCTGCGTGGTCAGCGCACCGCCGAGCTGGGAGCGGGTGTCTTCCTCCGT
CCGGACGAGGTCGACGCCGACACCCTCTGGCAGGCTCTCGCCACAGTGGTCGAGGACCGT
TCCCACGCCGAGAACGCCGAGAAGCTGCGGCAGGAGGCGCTGGCCGCGCCGACCCCCGCC
GAGGTCGTACCCGTGCTGGAGGCGCTCGCCCACCAGCACCGCGCCGACAGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR004276 Glycosyl transferase, family 28 (Domain) PF03033 Glyco_transf_28 IPR010610 Domain of unknown function DUF1205 (Domain) PF06722 DUF1205
SignalP	Eukaryota Bacteria, Gram-negative Bacteria, Gram-positive
TMHMM	No significant hit

Megalo_00090 Megalo_00110