Megalo_00160 : CDS information

Location

Organism	Micromonospora megalomicea subsp. nigra
Strain	NRRL 3275 (=NBRC 14744)
Entry name	Megalomicin
Contig	AF263245
Start / Stop / Direction	44,355 / 45,623 / + [in whole cluster] 44,355 / 45,623 / + [in contig]
Location	44355..45623 [in whole cluster] 44355..45623 [in contig]
Type	CDS
Length	1,269 bp (422 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	dTDP-D-desosamine glycosyltransferase
Product (GenBank)	TDP-desosamine glycosyltransferase
Gene
Gene (GenBank)	megCIII
EC number
Keyword	desosamine
Note
Note (GenBank)
Reference	ACC Q9F826 PmId [10972798] Biosynthesis of the anti-parasitic agent megalomicin: transformation of erythromycin to megalomicin in Saccharopolyspora erythraea. (Mol Microbiol. , 2000) [16514147] In vivo characterization of the dTDP-D-desosamine pathway of the megalomicin gene cluster from Micromonospora megalomicea. (Microbiology. , 2006) [20418422] TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli. (Appl Environ Microbiol. , 2010) comment [PMID: 10972798](2000) megalomicin (meg) biosynthetic gene clusterの報告。 megCIII: glycosyltransferase aglycon(3-alpha-mycarosyl-erythronolide B)のC5へdesosamineを付加するglycosyltransferase. erythromycin合成におけるdesosaminyltransferase EryCIIIに似ている。 megDIも似ているがmegCIIIのほうが似ているのでこっちをdesosaminyltransferase、megDIをproposed megosaminyltransferaseとしている。 --- [PMID: 16514147](2006) 3-alpha-mycarosylerythronolide BへのdTDP-D-desosamineのvivoでの移動のためにglycosyltransferase MegCIIIがMegCIIを必要とすることをBioconversion experimentsで実証している。 --- また[PMID: 20418422](2010)においてmeg clusterに存在するもうひとつのamino-sugar transferase megDI/megDVIとの関連も確認したところ、glycosyltransferaseについては基質特異的（MegCIIIはD-desosamineに、MegDIはL-megosamineに）だが、helper protein MegCII, MegDVIはどちらのglycosyltransferasesにも有効であった。

Sequence

>dTDP-D-desosamine glycosyltransferase [TDP-desosamine glycosyltransferase]
MRVVFSSMASKSHLFGLVPLAWAFRAAGHEVRVVASPALTDDITAAGLTAVPVGTDVDLV
DFMTHAGYDIIDYVRSLDFSERDPATSTWDHLLGMQTVLTPTFYALMSPDSLVEGMISFC
RSWRPDWSSGPQTFAASIAATVTGVAHARLLWGPDITVRARQKFLGLLPGQPAAHREDPL
AEWLTWSVERFGGRVPQDVEELVVGQWTIDPAPVGMRLDTGLRTVGMRYVDYNGPSVVPD
WLHDEPTRRRVCLTLGISSRENSIGQVSVDDLLGALGDVDAEIIATVDEQQLEGVAHVPA
NIRTVGFVPMHALLPTCAATVHHGGPGSWHTAAIHGVPQVILPDGWDTGVRAQRTEDQGA
GIALPVPELTSDQLREAVRRVLDDPAFTAGAARMRADMLAEPSPAEVVDVCAGLVGERTA
VG

>dTDP-D-desosamine glycosyltransferase [TDP-desosamine glycosyltransferase]
ATGCGCGTCGTCTTCTCCTCCATGGCCAGCAAGAGCCACCTGTTCGGTCTCGTTCCCCTC
GCCTGGGCCTTCCGCGCGGCGGGCCACGAGGTACGGGTCGTCGCCTCACCGGCTCTCACC
GACGACATCACGGCGGCCGGACTGACGGCCGTACCGGTCGGCACCGACGTCGACCTTGTC
GACTTCATGACCCACGCCGGGTACGACATCATCGACTACGTCCGCAGCCTGGACTTCAGC
GAGCGGGACCCGGCCACCTCCACCTGGGACCACCTGCTCGGCATGCAGACCGTCCTCACC
CCGACCTTCTACGCCCTGATGAGCCCGGACTCGCTGGTCGAGGGCATGATCTCCTTCTGT
CGGTCGTGGCGACCCGACTGGTCGTCTGGACCGCAGACCTTCGCCGCGTCGATCGCGGCG
ACGGTGACCGGCGTGGCCCACGCCCGACTCCTGTGGGGACCCGACATCACGGTACGGGCC
CGGCAGAAGTTCCTCGGGCTGCTGCCCGGACAGCCCGCCGCCCACCGGGAGGACCCCCTC
GCCGAGTGGCTCACCTGGTCTGTGGAGAGGTTCGGCGGCCGGGTGCCGCAGGACGTCGAG
GAGCTGGTGGTCGGGCAGTGGACGATCGACCCCGCCCCGGTCGGGATGCGCCTCGACACC
GGGCTGAGGACGGTGGGCATGCGCTACGTCGACTACAACGGCCCGTCGGTGGTGCCGGAC
TGGCTGCACGACGAGCCGACCCGCCGACGGGTCTGCCTCACCCTGGGCATCTCCAGCCGG
GAGAACAGCATCGGGCAGGTCTCCGTCGACGACCTGTTGGGTGCGCTCGGTGACGTCGAC
GCCGAGATCATCGCGACAGTGGACGAGCAGCAGCTCGAAGGCGTCGCCCACGTCCCGGCC
AACATCCGTACGGTCGGGTTCGTCCCGATGCACGCACTGCTGCCGACCTGCGCGGCGACG
GTGCACCACGGCGGTCCCGGCAGCTGGCACACCGCCGCCATCCACGGCGTGCCGCAGGTG
ATCCTGCCCGACGGCTGGGACACCGGGGTCCGCGCCCAGCGGACCGAGGACCAGGGGGCG
GGCATCGCCCTGCCGGTGCCCGAGCTGACCTCCGACCAGCTCCGCGAGGCGGTGCGGCGG
GTCCTGGACGATCCCGCCTTCACCGCCGGTGCGGCGCGGATGCGGGCCGACATGCTCGCC
GAGCCGTCCCCCGCCGAGGTCGTCGACGTCTGTGCGGGGCTGGTCGGGGAACGGACCGCC
GTCGGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR004276 Glycosyl transferase, family 28 (Domain) PF03033 Glyco_transf_28 IPR010610 Domain of unknown function DUF1205 (Domain) PF06722 DUF1205
SignalP	Eukaryota Bacteria, Gram-positive Bacteria, Gram-negative
TMHMM	No significant hit

Megalo_00150 Megalo_00170