MUP_00600 : CDS information

Location

Organism	Mycobacterium ulcerans
Strain	Agy99
Entry name	Mycolactone
Contig	BX649209
Start / Stop / Direction	155,147 / 155,668 / + [in whole cluster] 155,147 / 155,668 / + [in contig]
Location	155147..155668 [in whole cluster] 155147..155668 [in contig]
Type	CDS
Length	522 bp (173 aa)

Annotation

Category
Product
Product (GenBank)	probable transposase for the insertion element IS2606 (fragment)
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	MUP060, -, len: 173 aa. Probable IS2606 transposase fragment, highly similar to the N-terminus of Q9ZG97 Probable transposase from Mycobacterium ulcerans (444 aa), fasta scores: opt: 886, E(): 1.8e-52, (84.615% identity in 169 aa overlap); and to the N-terminus of O05912 Hypothetical 48.7 kDa protein (IS1554, transposase) from M. tuberculosis (439 aa), fasta scores: opt: 506, E(): 1.1e-26, (60.000% identity in 135 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses MUP060 and MUP061, no sequence errors have been identified to account for this.

Sequence

>[probable transposase for the insertion element IS2606 (fragment)]
MMTKTVVAVQPSQNHEDEAGAVAAIDVPSSRDVDELEVARELVRQAREAGVGLTGPGGLL
KAMTKTVIEIALDEELSEHLGYDRHDRAGYGSGNSRNGTRSKTVLTDGCGFIEIDVPRDR
AGTFEPKIVEKRQRRLTDVDEVGVVAVCPRVDHRGDQRPFRPHLRRGGVQGHR

>[probable transposase for the insertion element IS2606 (fragment)]
ATGATGACCAAGACTGTCGTGGCTGTGCAGCCATCACAAAACCACGAAGATGAGGCCGGC
GCCGTGGCGGCTATTGACGTTCCCAGTAGTCGCGATGTTGATGAGTTAGAAGTCGCCCGC
GAGTTGGTGCGCCAGGCCCGCGAGGCCGGCGTTGGGTTAACCGGGCCTGGCGGATTGCTC
AAGGCGATGACCAAAACGGTCATTGAGATCGCTTTGGATGAGGAACTGTCCGAGCACCTT
GGATATGACCGCCACGATCGGGCTGGGTATGGCAGTGGCAACTCGCGCAACGGAACCCGC
TCGAAAACGGTGCTCACTGATGGGTGCGGTTTCATTGAGATCGATGTCCCACGGGATCGG
GCAGGCACATTCGAGCCGAAGATCGTCGAAAAACGCCAGCGTAGGTTGACCGACGTCGAT
GAGGTGGGTGTTGTCGCTGTATGCCCGCGGGTTGACCACCGGGGAGATCAGCGCCCATTT
CGCCCACATCTACGACGCGGCGGTGTCCAAGGACACCGTTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001207 Transposase, mutator type (Family) PF00872 Transposase_mut
SignalP	No significant hit
TMHMM	No significant hit

MUP_00590 MUP_00610