Nanch_00210 Nanch_00210   Nanch_00230 Nanch_00230

Nanch_00220 : CDS information

close this sectionLocation

Organism
StrainNS3226
Entry nameNanchangmycin
Contig
Start / Stop / Direction78,263 / 77,949 / - [in whole cluster]
78,263 / 77,949 / - [in contig]
Locationcomplement(77949..78263) [in whole cluster]
complement(77949..78263) [in contig]
TypeCDS
Length315 bp (104 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)NanA10
Gene
Gene (GenBank)nanA10
EC number
Keyword
  • modification base
Note
  • It is proposed that this module is not involved in the extension of polyketide chain, but provides a base for modification.
Note (GenBank)
  • ACP
Reference
ACC
PmId
[12770825] A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. (Chem Biol. , 2003)
comment
nanchangmycin生合成のためのcomplete gene clusterの報告。
（complete clusterと言っているが、同定されたclusterがNanchangmycin生合成に十分かは確認されていない。）

NanA10: PKS (ACP)

nanA9は不完全moduleでKS and AT domainしかないが、単一ACPを持つ独立したORF=nanA10がnanA9の下流（隣ではない）にある。nanA9とA10の間には、PKSではないがnanchangmycin生合成に必要なgenesが2つ存在している。

このACP独立と、一緒に並ぶ遺伝子の構成から、module 13での鎖延伸の後、NanA10-ACPに繋がれたままpolyether構造形成が行われ、それからmodule 14での鎖延伸が起こる、ということが示唆される。

close this sectionPKS/NRPS Module

13
ACP22..96

close this sectionSequence

selected fasta
>polyketide synthase [NanA10]
MEPNEEATHDEQLVARLASATPEERQRLLAAQVLRRASEVLDVPALDEESNFLENGLSSL
SAVQLAKSLMSDTGLEVPLVAIVEHPTSTLLGKYLAETYEADAA
selected fasta
>polyketide synthase [NanA10]
ATGGAACCCAACGAGGAAGCCACTCACGACGAGCAGCTGGTCGCACGTCTCGCGTCCGCG
ACGCCGGAGGAACGCCAGCGGCTGCTGGCCGCGCAGGTGCTCCGCCGCGCCTCCGAGGTC
CTCGACGTCCCCGCCCTGGACGAGGAGAGCAACTTCCTGGAGAACGGCCTGAGCTCGCTG
AGCGCCGTGCAACTGGCCAAGAGCCTGATGAGCGACACCGGGCTCGAAGTCCCCCTCGTC
GCGATCGTCGAACACCCCACCTCCACCCTGCTCGGCAAGTACCTCGCGGAGACCTACGAA
GCGGATGCGGCCTAG
[13] ACP22..96
[13] ACP64..288

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR009081 Acyl carrier protein-like (Domain)
[22-96] 15.462 PS50075
PS50075   ACP_DOMAIN
[18-100] 1.9e-13 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   no description
[22-97] 2.3e-12 SSF47336
SSF47336   ACP-like
[32-87] 1.3e-07 PF00550
PF00550   PP-binding
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[30-99] 7.6e-06 SM00823
SM00823   Phosphopantetheine attachment site
SignalP No significant hit
TMHMM No significant hit
Nanch_00210 Nanch_00210   Nanch_00230 Nanch_00230
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