Nanch_00230 : CDS information

Location

Organism	Streptomyces nanchangensis
Strain	NS3226
Entry name	Nanchangmycin
Contig	AF521085
Start / Stop / Direction	79,763 / 78,327 / - [in whole cluster] 79,763 / 78,327 / - [in contig]
Location	complement(78327..79763) [in whole cluster] complement(78327..79763) [in contig]
Type	CDS
Length	1,437 bp (478 aa)

Annotation

Category	3.3 modification reduction
Product	putative epoxidase
Product (GenBank)	NanO
Gene
Gene (GenBank)	nanO
EC number
Keyword
Note
Note (GenBank)	putative epoxidase
Reference	ACC Q7WTE0 PmId [12770825] A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. (Chem Biol. , 2003) comment nanchangmycin生合成のためのcomplete gene clusterの報告。（complete clusterと言っているが、同定されたclusterがNanchangmycin生合成に十分かは確認されていない。）配列解析からの機能予測 NanO: Epoxidase NanOはdiene中間体の2つのepoxidationsを実行する候補。
Related Reference	ACC Q846W9 NITE Monen_00220 PmId [12940979] Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. (Mol Microbiol. , 2003) comment 3rd(Q846W9) 59%, 1e-159 Streptomyces cinnamonensis_monCI monensin epoxidase monCIのS. coelicolorにおける異種性発現で、linaloolをepoxidizeする能力が10-20倍に増加することを確認している。

Sequence

>putative epoxidase [NanO]
MTTPTRAVVLGGGWAGMLTAHVLARHLESVTVVERDILPDGPHHRKGQPQARHVHVLWSS
GAGIVENLLPGTAERLLAAGARRIGFQSDLVTLTAWGWQYRFPATAYAMMCTRPLLDWVV
RDAILAGGRIEVEHGTEAVELAGDRSRVTGVRVRDAGGGEPRLLEADLVVDATGRASRLG
HWLAALGLPAVEQDVVDAGIGYATRMFKAPEGADGNFPAVQVAADPLTRQPGRFGVVYPQ
EGGRWLVTLTSTRGAPLPTDEDEFTGYAKVLRHSIVSELMSVAEPISPIFQSHSGANRRM
YPERMPQWPEGLLILGDSLAAFNPVYGHGMSSAARAAEALDKELARDGFGEGGTRQVQRA
LSEVVDDPWIMAGLNDIQYVNCRNLSSDPRLTGPDVAERLKFSDFLSGKSIRSPKVCEVT
TSVLSLNAPQKALGDSRFLSLLRTDTSHPKLVEPPFHPEELEMVGLKPSGIAAKGALG

>putative epoxidase [NanO]
ATGACCACACCGACACGTGCCGTTGTGCTGGGCGGCGGATGGGCCGGCATGCTCACCGCC
CATGTGCTGGCCCGCCACCTGGAATCCGTGACCGTGGTGGAGCGTGACATCCTGCCCGAC
GGACCGCACCACCGTAAGGGACAGCCCCAGGCCCGCCACGTCCACGTCCTGTGGTCCAGT
GGCGCGGGCATCGTCGAGAACCTGCTGCCGGGCACCGCCGAACGGCTGCTGGCGGCCGGT
GCCCGCCGGATCGGCTTCCAGAGCGACCTGGTGACCCTGACGGCGTGGGGCTGGCAGTAC
CGCTTCCCGGCCACCGCGTACGCGATGATGTGCACCCGCCCCCTGCTCGACTGGGTGGTG
CGTGACGCGATCCTCGCCGGCGGCCGGATCGAGGTGGAGCACGGCACCGAGGCCGTCGAG
CTGGCCGGGGACCGCTCCAGGGTCACCGGGGTGCGGGTGCGGGACGCGGGCGGCGGCGAA
CCCAGGCTGCTGGAGGCCGACCTGGTGGTGGACGCCACGGGGCGCGCCTCGCGCCTGGGC
CACTGGCTGGCGGCCCTCGGCCTGCCGGCCGTCGAGCAGGACGTGGTCGACGCCGGCATC
GGCTACGCGACCCGCATGTTCAAGGCGCCCGAGGGCGCGGACGGAAACTTCCCCGCGGTC
CAGGTCGCCGCCGACCCCCTCACCCGGCAACCGGGCCGCTTCGGCGTGGTCTACCCGCAG
GAGGGCGGTCGCTGGCTGGTCACCCTGACCAGCACGCGCGGCGCCCCGCTGCCCACCGAC
GAGGACGAGTTCACCGGCTACGCCAAGGTCCTGCGGCACTCGATCGTCAGCGAACTCATG
AGCGTCGCCGAGCCCATCAGCCCCATCTTCCAGTCGCACTCGGGAGCCAACCGGCGCATG
TACCCCGAGCGCATGCCGCAGTGGCCGGAGGGCCTGCTGATCCTCGGCGACTCGCTGGCG
GCGTTCAATCCGGTCTACGGCCACGGCATGAGCTCGGCGGCCCGTGCCGCCGAGGCCCTG
GACAAGGAGCTGGCACGCGACGGCTTCGGCGAGGGCGGCACCCGGCAGGTCCAGCGCGCG
CTGAGCGAGGTGGTGGACGATCCCTGGATCATGGCCGGGCTGAACGACATCCAGTACGTC
AACTGCCGCAACCTCTCCAGCGACCCGCGGCTGACCGGCCCGGACGTCGCGGAGCGGCTG
AAGTTCTCCGACTTCCTCTCCGGCAAGTCGATCCGCTCGCCGAAGGTGTGCGAGGTCACC
ACCAGCGTGCTGAGCCTGAACGCGCCGCAGAAGGCCCTGGGCGACTCCCGCTTCCTGTCG
TTGCTGCGCACCGACACCAGCCACCCGAAGCTCGTCGAACCGCCGTTCCACCCCGAGGAG
CTGGAGATGGTCGGGCTCAAGCCGTCCGGCATCGCCGCGAAGGGCGCGCTCGGCTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002938 Monooxygenase, FAD-binding (Domain) PF01494 FAD_binding_3
SignalP	Eukaryota Bacteria, Gram-positive Bacteria, Gram-negative
TMHMM	No significant hit

Nanch_00220 Nanch_00240