Nysta_00100 : CDS information

Location

Organism	Streptomyces noursei
Strain	ATCC 11455 (=NBRC 15452)
Entry name	Nystatin
Contig	AF263912
Start / Stop / Direction	59,833 / 58,637 / - [in whole cluster] 59,833 / 58,637 / - [in contig]
Location	complement(58637..59833) [in whole cluster] complement(58637..59833) [in contig]
Type	CDS
Length	1,197 bp (398 aa)

Annotation

Category	3.3 modification reduction
Product	cytochrome P450
Product (GenBank)	NysN
Gene
Gene (GenBank)	nysN
EC number
Keyword	C-16 carboxy group formation
Note
Note (GenBank)	P450 monooxygenase
Reference	ACC Q9L4W8 PmId [10873841] Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway. (Chem Biol. , 2000) [21764946] New nystatin-related antifungal polyene macrolides with altered polyol region generated via biosynthetic engineering of Streptomyces noursei. (Appl Environ Microbiol. , 2011) comment [PMID: 10873841](2000) Nystatin(nys) biosynthetic gene clusterの報告。 nysN: P450 monooxygenase @oxidation at methyl group ac C-16 nysL and nysN genesはP450 monooxygenasesをコードする。これらのproductはnystatin polyketide moietyのC10 hydroxylationか、C16 methyl基のoxidationを担う。 -- [PMID:21764946](2011) 遺伝子操作による新規化合物の抗真菌活性、溶血作用を調査。 NysN不活化のあるmutantsではC-16がメチル基のまま。

Sequence

>cytochrome P450 [NysN]
MSTEADARTAAPQCPVAFPLRRPGRPFPPPEYATYRGGAGLVRSELPSGPVWLVTRHEDV
RAVLTDPRISADPSKPGFPKAGRTGGAPSQYEVPGWFVAMDPPEHGRFRKTLIPEFTVRK
VRELRPVIQQIVDERIDAMLAAGTSADLVESFALPVPSLVISSLLGVPKVDRDFFEDRTR
VLVRLSSTDEERDKATQALLRYLGRLIQIKQRRPGDDLISRLIAAGTLSRQELSGVAMLL
LIAGHETTANNIGLGVVQLLTNPRWIGDDRIVEELLRYYSVADLVAFRVAVEDVEIGGQL
IRAGEGIVPLIAAANHDATAFAAPSEFDPERSARSHVAFGYGVHQCLGQNLVREEMDIAY
RTLFARIPSLTLAVPVEELPLKYDGVLFGLHELPVTWK

>cytochrome P450 [NysN]
ATGAGCACCGAAGCCGACGCGCGGACCGCCGCGCCGCAGTGCCCGGTGGCCTTCCCGCTG
CGCAGGCCGGGCCGGCCCTTCCCCCCGCCGGAGTACGCGACCTACCGCGGCGGGGCGGGC
CTGGTCCGCTCCGAACTCCCCAGCGGCCCGGTCTGGTTGGTGACCCGGCACGAGGACGTC
CGGGCGGTGCTGACCGACCCCCGGATCAGCGCCGACCCCTCCAAACCCGGTTTCCCCAAG
GCCGGTCGGACCGGTGGGGCCCCGTCGCAGTACGAGGTGCCGGGGTGGTTCGTGGCGATG
GACCCACCGGAGCACGGCCGGTTCCGCAAGACCTTGATCCCCGAGTTCACCGTGCGCAAG
GTGCGGGAACTGCGGCCGGTGATCCAGCAGATCGTGGACGAGCGGATCGACGCGATGCTG
GCCGCGGGCACCTCGGCGGACCTCGTCGAGTCCTTCGCGCTGCCGGTGCCGTCCCTGGTG
ATCTCCAGTCTGCTCGGGGTGCCCAAGGTCGACCGGGACTTCTTCGAGGACCGGACCCGG
GTGCTGGTCCGGCTCAGTTCGACCGACGAGGAGCGCGACAAGGCGACCCAGGCGCTGCTG
CGCTATCTGGGCCGACTCATCCAGATCAAGCAGCGGCGACCCGGTGACGACCTGATCAGC
CGGCTGATCGCCGCGGGCACACTGTCCCGGCAGGAGCTGTCCGGGGTGGCGATGCTGCTG
CTGATCGCCGGGCACGAGACGACGGCGAACAACATCGGCCTGGGCGTGGTGCAGTTGCTC
ACCAACCCGCGGTGGATCGGCGACGACCGGATCGTCGAGGAACTGCTGCGCTACTACTCG
GTGGCCGATCTGGTGGCATTCCGGGTGGCCGTCGAGGACGTGGAGATCGGCGGTCAGCTG
ATCCGGGCCGGCGAGGGCATCGTGCCGCTGATCGCCGCCGCCAACCACGACGCCACGGCC
TTCGCGGCGCCCTCGGAGTTCGACCCCGAGCGCTCGGCGCGCTCGCACGTGGCGTTCGGC
TACGGTGTCCACCAGTGCCTGGGCCAGAACCTGGTGCGGGAGGAGATGGACATCGCCTAC
CGCACGCTCTTCGCCCGGATCCCCTCCCTCACGCTCGCCGTGCCGGTGGAGGAACTGCCG
TTGAAGTACGACGGGGTGCTGTTCGGCCTGCACGAACTGCCCGTCACCTGGAAGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PF00067 p450 PR00385 P450 G3DSA:1.10.630.10 Cyt_P450 SSF48264 Cytochrome_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

Nysta_00090 Nysta_00110