Pyolu_00120 Pyolu_00120   Pyolu_00140 Pyolu_00140

Pyolu_00130 : CDS information

close this sectionLocation

Organism
StrainPf-5
Entry namePyoluteorin
Contig
Start / Stop / Direction24,462 / 26,231 / + [in whole cluster]
24,462 / 26,231 / + [in contig]
Location24462..26231 [in whole cluster]
24462..26231 [in contig]
TypeCDS
Length1,770 bp (589 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative ABC transporter ATP-binding protein
Product (GenBank)PltJ
GenepltJ
Gene (GenBank)PltJ
EC number
Keyword
  • type I secretion system
Note
Note (GenBank)
  • putative ATP-binding cassette of ABC transporter
Reference
ACC
PmId
[16269724] Reciprocal regulation of pyoluteorin production with membrane transporter gene expression in Pseudomonas fluorescens Pf-5. (Appl Environ Microbiol. , 2005)
comment
pyoluteorin生合成clusterの3'側 pltZ-orf1についての論文
pltJ: cytoplasmic ATP-binding cassette of ABC transporter

pltI or pltJ mutantでpyoluteorin産生は非常に減るが、細胞内部のpyoluterin蓄積が比例して増えるわけではなかった。
よってpyoluteorinの独占的なexporterではなさそう。
pltI and pltJの転写は外因性pyoluteorinによって拡大する。
Related Reference
ACC
Q4VWC9
PmId
[16581203] Identification and characterization of a putative ABC transporter PltHIJKN required for pyoluteorin production in Pseudomonas sp. M18. (Gene. , 2006)
comment
4th(Q4VWC9) 76%, 0.0
Pseudomonas sp. M18_pltI
ATP-binding protein

Pseudomonas sp. M18もPyoluteorinを産生する。
gene nameはP. fluorescens Pf-5と一致していない。

---
配列から、pltHIJKN gene productsが典型的な3つの要素のABC export systemに組み込まれることが示唆される。
そのシステムは、
・inner membrane ABC transporter PltIJK
・membrane fusion protein PltH
・outer membrane efflux protein PltN
からなる。

pltH or pltI 欠陥mutantではPlt非産生。
pltHIJKN gene clusterの過剰発現でPlt産生増加。
pltHIJKN gene clusterの異種性発現で外因性Pltへの抵抗性あり。
外因性Pltは、Plt生合成gene clusterとABC transporter gene cluster pltHIJKN の両方の発現を転写レベルで誘導する。

close this sectionSequence

selected fasta
>putative ABC transporter ATP-binding protein [PltJ]
MSAPAVTVQAVELVKRFRSGTREQLALEQVSLEVPSGKLSALVGPDGAGKTTLLRMIAGL
LKADEGLLKVLGLDVAADPQQVQDLISYMPQKFGLYEDLTIQENLELYADLHGVRAQQRE
ERFSRLLQMMDLTRFRDRLAGQLSGGMKQKLGLACTLVRSPQLLLLDEPTVGVDPLSRRE
LWSIIEQLIEQENLTVLISTAYMDEAQRCAQVFVLYQGRLIAQGPPAQLCHLAQGLCYGV
GPEAGTPARLLQARLLDDNEHIIDAVPEGGMVRFIRSAQVPLQAIPSLAGQASPQALEAR
LEDGFMVLLRQQVGSVASLDEPAQAPASYGQGDAASGRAVVIEVRDLVRKFGDFTAVAST
SFEVLKGEIFGLLGPNGAGKTTTFRMLCGLLPASSGYLEVAGVNLRTARAAARRKIGYVS
QKFALYANLSALENLRFFGGAYGLHGAKLKARVALMLEQFDLDEHKHLRSGELPGGYKQR
LAMAVALLHEPQILFLDEPTSGIDPLARRAFWRRITALAQSGTTIVITTHFMEEAEYCDR
IVIQDAGRLLALGTPEQVRHQGSESLADMNSAFIAVVERARAQASAPVQ
selected fasta
>putative ABC transporter ATP-binding protein [PltJ]
ATGAGTGCGCCAGCGGTAACGGTTCAGGCTGTTGAACTGGTCAAGCGCTTTCGCAGTGGA
ACGCGAGAACAGTTGGCTCTGGAGCAGGTTTCTCTGGAGGTGCCCAGTGGCAAGTTGAGT
GCCCTGGTGGGGCCGGACGGGGCCGGAAAAACCACCCTGTTGCGGATGATTGCCGGCTTG
CTCAAGGCCGACGAAGGGCTGTTGAAGGTGCTGGGGCTGGACGTCGCCGCAGATCCGCAG
CAGGTGCAGGACCTCATCAGCTACATGCCCCAGAAGTTCGGCCTGTACGAAGACCTGACG
ATCCAGGAAAACCTCGAACTCTACGCCGACCTGCATGGCGTGCGCGCCCAGCAGCGCGAG
GAGCGCTTTTCCCGGTTGCTGCAGATGATGGACCTGACGCGTTTTCGCGACCGGCTGGCC
GGACAGCTTTCCGGCGGCATGAAACAGAAACTGGGGCTGGCCTGTACCCTGGTCCGTTCA
CCGCAACTGTTGCTGCTGGACGAGCCCACCGTGGGGGTGGATCCGCTGTCGCGGCGGGAA
CTGTGGAGCATCATCGAACAACTGATCGAGCAGGAAAACCTCACGGTGCTGATCAGCACT
GCCTACATGGACGAGGCCCAGCGTTGCGCCCAGGTCTTTGTGCTATACCAGGGGCGGCTG
ATCGCCCAGGGGCCACCGGCGCAGTTGTGCCATCTGGCGCAGGGCCTGTGCTATGGCGTC
GGCCCCGAGGCCGGCACCCCGGCCAGGCTGTTGCAGGCGCGCTTGCTTGACGACAATGAA
CACATCATCGATGCCGTGCCCGAAGGCGGCATGGTTCGCTTTATCCGTAGCGCCCAGGTG
CCTCTGCAGGCCATTCCATCCCTGGCTGGCCAGGCCTCTCCCCAGGCGCTGGAGGCCCGC
CTCGAAGATGGCTTCATGGTGTTGCTCCGGCAACAGGTTGGCAGCGTTGCAAGCCTGGAC
GAGCCGGCGCAGGCGCCCGCCAGCTACGGCCAGGGCGACGCTGCCTCTGGGCGTGCGGTG
GTCATCGAGGTCCGCGACCTGGTGCGCAAGTTCGGGGACTTCACCGCGGTTGCCAGCACC
TCGTTCGAGGTGTTGAAGGGCGAGATATTCGGCTTGCTGGGCCCCAACGGAGCCGGCAAG
ACCACCACGTTTCGCATGCTCTGCGGTTTGCTTCCCGCCTCCAGCGGGTACCTTGAGGTG
GCTGGCGTCAACCTGCGCACCGCACGGGCGGCGGCGCGGCGCAAGATCGGCTACGTCTCG
CAGAAGTTCGCCCTGTACGCGAACCTGTCGGCCCTGGAGAACCTGCGCTTCTTCGGCGGC
GCCTATGGCCTGCACGGCGCGAAGCTGAAAGCGCGGGTGGCGTTGATGCTCGAACAGTTC
GACCTCGACGAACACAAGCACCTGCGCAGCGGCGAGCTGCCCGGTGGCTACAAGCAGCGC
CTGGCCATGGCGGTCGCGCTGCTGCACGAGCCACAGATCCTGTTTCTCGACGAACCCACC
AGCGGTATCGACCCCTTGGCCCGCCGGGCGTTCTGGCGCCGTATCACCGCCCTGGCGCAA
AGCGGCACGACGATCGTGATCACCACCCACTTCATGGAAGAGGCCGAGTACTGCGACCGT
ATCGTCATCCAGGATGCCGGACGGCTGCTGGCCCTGGGCACTCCGGAACAGGTACGCCAC
CAGGGCAGCGAGTCGCTTGCCGACATGAACAGCGCCTTCATTGCCGTGGTCGAGCGTGCT
CGGGCCCAGGCCAGCGCCCCGGTGCAGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003439 ABC transporter-like (Domain)
[8-242] 22.489 PS50893 [342-571] 22.302 PS50893
PS50893   ABC_TRANSPORTER_2
[51-170] 3.6e-22 PF00005 [381-501] 4.5e-16 PF00005
PF00005   ABC_tran
IPR003593 AAA+ ATPase domain (Domain)
[36-218] 2.3e-10 SM00382 [366-547] 3.1e-16 SM00382
SM00382   ATPases associated with a variety of cellula
IPR017871 ABC transporter, conserved site (Conserved_site)
[143-157] PS00211
PS00211   ABC_TRANSPORTER_1
SignalP No significant hit
TMHMM No significant hit
Pyolu_00120 Pyolu_00120   Pyolu_00140 Pyolu_00140
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