Rifam_00270 Rifam_00270   Rifam_00290 Rifam_00290

Rifam_00280 : CDS information

close this sectionLocation

Organism
StrainS699
Entry nameRifamycin
Contig
Start / Stop / Direction69,003 / 70,058 / + [in whole cluster]
69,003 / 70,058 / + [in contig]
Location69003..70058 [in whole cluster]
69003..70058 [in contig]
TypeCDS
Length1,056 bp (351 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.2 modification addition of starter unit
Productputative aminodehydroquinate synthase
Product (GenBank)RifG
Gene
Gene (GenBank)rifG
EC number4.2.3.-
Keyword
  • AHBA
Note
Note (GenBank)
  • aminodehydroquinate synthase
Reference
ACC
PmId
[9512878] Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699. (Chem Biol. , 1998)
[11278540] Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699. (J Biol Chem. , 2001)
comment
[PMID: 9512878](1998)
ansamycin系抗生物質rifamycinの生合成gene clusterの報告。
RifG(336aa): Aminodehydroquinate synthase

RifGは、PKSのstarter unitのAHBA合成経路にあるaminoDAHP→aminoDHQの反応を触媒すると考えられている。

AHBA: 3-amino-5-hydroxybenzoic acid
aminoDAHP: 3,4-dideoxy-4-amino-D-arabino-heptulosonate 7-phosphate
aminoDHQ: 5-deoxy-5-amino-3-dehydroquinic acid

---
[PMID: 11278540](2001)
rifamycinと関連ansamycinsのPKS starter 3-amino-5-hydroxybenzoic acid (AHBA)の生合成経路について調査。関連遺伝子の不活化、異種性発現、中間体を基質とした反応確認など。

RifG: Aminodehydroquinate synthase

rifH, -G, and -J genesの遺伝子産物は shikimate biosynthesis pathwayに関連する酵素に似ている。rifH, G, Jの各mutantは成長に影響を与えないので、shikimate pathwayに直接関連しないことがわかる。また、mutantでもrifamycin B産生をある程度保つことから、shikimate pathwayのgeneが代替していると考えられる。

AHBA biosynthetic genesをプラスミドに組み込んだS. coelicolor YU105を使ってAHBA生産を調べると、rifGを除いた場合はAHBA biosynthetic genesが全て揃っている場合と比較して、AHBAの生産量が約10%にまで落ちることが示されており、rifGはAHBAの生産に関与していることが示されている。
Related Reference
ACC
P0A4Z4
PmId
[17586643] Functional characterization by genetic complementation of aroB-encoded dehydroquinate synthase from Mycobacterium tuberculosis H37Rv and its heterologous expression and purification. (J Bacteriol. , 2007)
[1910148] The Mycobacterium tuberculosis shikimate pathway genes: evolutionary relationship between biosynthetic and catabolic 3-dehydroquinases. (Mol Gen Genet. , 1991)
comment
127th, id44%, 9e-50
Mycobacterium tuberculosis_aroB
3-dehydroquinate synthase(EC 4.2.3.4)

[PMID: 17586643]
Mycobacterium tuberculosis H37Rv_aroBをクローニング、E. coliで大量発現してhomogeneityに精製、アミノ酸シークエンス。M. tub_aroBはE. coli aroB deletion mutantを相補することを確認。

close this sectionSequence

selected fasta
>putative aminodehydroquinate synthase [RifG]
MRTTIPVRLAERSYDVLVGPGVRAALPEVVRRLGARRAVVVSARPADWVPGTGVETLLLQ
ARDGEPTKRLSTVEELCGEFARFGLTRSDVVVSCGGGTTTDVVGLAAALYHRGVAVVHLP
TSLLAQVDASVGGKTAVNLPAGENLVGAYWQPSAVLCDTDYLTTLPRREVLNGLGEIARC
HFIGAPDLRGRSRPEQIAASVTLKAGIVAQDERDTGPRHLLNYGHTLGHALEIATGFALR
HGEAVAIGTVFAGRLAGALGRLDQSGVDEHLAVVRHYGLPAALPADVDPAVLVRQMYRDK
KAITGLAFVLAGPRGAELVSDVPAPVVTDVLDRMPRDSLENLVGTTEAAAP
selected fasta
>putative aminodehydroquinate synthase [RifG]
GTGAGGACGACGATCCCGGTCCGCCTGGCGGAACGGTCCTACGACGTGCTCGTCGGCCCC
GGGGTGCGGGCGGCGCTGCCCGAGGTCGTCCGGCGGCTCGGCGCGAGACGGGCCGTGGTC
GTGTCGGCCCGGCCGGCGGACTGGGTGCCCGGCACCGGCGTCGAGACCCTGCTGCTCCAG
GCGCGCGACGGCGAGCCGACCAAGCGGCTGTCCACAGTGGAGGAACTGTGCGGTGAGTTC
GCGCGGTTCGGGCTCACCCGGTCCGACGTCGTGGTCTCCTGCGGCGGCGGCACGACCACG
GACGTCGTCGGGCTCGCGGCCGCGCTGTACCACCGGGGGGTCGCCGTGGTCCACCTGCCC
ACGTCCCTGCTCGCCCAGGTCGACGCCAGCGTCGGCGGGAAGACCGCGGTGAACCTGCCG
GCGGGCGAGAACCTCGTCGGGGCGTACTGGCAGCCCAGCGCGGTGCTGTGCGACACGGAC
TACCTGACGACGCTGCCGCGGCGGGAGGTGCTGAACGGCCTCGGCGAGATCGCCCGCTGC
CACTTCATCGGCGCGCCGGACCTGCGGGGGCGCTCGCGCCCGGAGCAGATCGCCGCCAGC
GTCACCCTCAAGGCGGGCATCGTCGCGCAGGACGAGCGGGACACCGGCCCGCGGCACCTG
CTCAACTACGGCCACACGCTGGGGCACGCGCTGGAGATCGCGACCGGCTTCGCCCTGCGC
CACGGCGAGGCGGTGGCGATCGGCACGGTCTTCGCGGGCCGGCTGGCCGGCGCGCTCGGC
CGCCTCGACCAGTCCGGTGTGGACGAACACCTCGCCGTCGTCCGCCACTACGGCCTGCCC
GCCGCGCTGCCCGCGGACGTCGACCCGGCGGTGCTCGTCCGGCAGATGTACCGGGACAAG
AAGGCGATCACCGGGCTCGCCTTCGTCCTGGCCGGGCCGCGGGGCGCGGAGCTGGTGAGC
GACGTGCCGGCGCCGGTCGTCACCGACGTCCTGGACCGGATGCCCCGCGACAGCCTGGAA
AACCTGGTGGGGACGACGGAAGCGGCGGCGCCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR016037 3-dehydroquinate synthase AroB (Family)
[3-339] 1.3000049540733e-79 PIRSF001455
PIRSF001455   DHQ_synth
SignalP
[1-43] 0.228 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Rifam_00270 Rifam_00270   Rifam_00290 Rifam_00290
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