Rifam_00280 Rifam_00280   Rifam_00300 Rifam_00300

Rifam_00290 : CDS information

close this sectionLocation

Organism
StrainS699
Entry nameRifamycin
Contig
Start / Stop / Direction70,055 / 71,380 / + [in whole cluster]
70,055 / 71,380 / + [in contig]
Location70055..71380 [in whole cluster]
70055..71380 [in contig]
TypeCDS
Length1,326 bp (441 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.2 modification addition of starter unit
Product3,4-dideoxy-4-amino-D-arabino-heptulosonate 7-phosphate synthase
aminoDAHP synthase
Product (GenBank)RifH
Gene
Gene (GenBank)rifH
EC number
Keyword
  • AHBA
Note
Note (GenBank)
  • aminoDAHP synthase
Reference
ACC
PmId
[9512878] Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699. (Chem Biol. , 1998)
[11278540] Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699. (J Biol Chem. , 2001)
[11804477] Biosynthesis of 1-deoxy-1-imino-D-erythrose 4-phosphate: a defining metabolite in the aminoshikimate pathway. (J Am Chem Soc. , 2002)
comment
[PMID: 9512878](1998)
ansamycin系抗生物質rifamycinの生合成gene clusterの報告。
RifH(441aa): aminoDAHP synthase

RifHは、rifamycin PKSのstarter unitであるAHBAの合成過程で、aminoDAHP(3,4-dideoxy-4-amino-D-arabino-heptulosonate 7-phosphate)の生成を触媒すると考えられている。

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[PMID: 11278540](2001)
rifamycinと関連ansamycinsのPKS starter 3-amino-5-hydroxybenzoic acid (AHBA)の生合成経路について調査。関連遺伝子の不活化、異種性発現、中間体を基質とした反応確認など。

RifH: aminoDAHP synthase

rifH, -G, and -J genesの遺伝子産物は shikimate biosynthesis pathwayに関連する酵素に似ている。rifH, G, Jの各mutantは成長に影響を与えないので、shikimate pathwayに直接関連しないことがわかる。また、mutantでもrifamycin B産生をある程度保つことから、shikimate pathwayのgeneが代替していると考えられる。

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[PMID: 11804477](2002)
starter AHBAの前駆体aminoDAHP合成が、3-amino-3-deoxy-D-fructose 6-phosphate(aminoF6P)に由来することを確認している。

aminoF6P, D-ribose 5-phosphate(R5P), phosphoenolpyruvate(PEP), E.coli transketolase_TktA, A. mediterranei RifH aminoDAHP synthase(64units)で反応すると、aminoDAHP, DAHPを産生。
使用しているRifHはFloss HG.提供によるplasmidから精製している。
aminoDAHP synthase activityはDAHP synthase activityとして測定されたもの。

aminoF6P, R5P, PEP, A. mediterranei ATCC21789 crude cell lysate(DAHP synthase activity 0.2units)で反応すると、aminoDAHP, DAHP, AHBA, Tyr, Pheを産生。

[6,6-2H, amino-15N]aminoF6Pを使った実験で、aminoF6PのframeworkとNがaminoDAHPに取り込まれることが実証された。

提唱経路は、
aminoF6P + R5P → 1-deoxy-1-imino-D-erythrose 4-phosphate(iminoE4P)
iminoE4P + PEP → aminoDAHP
(aminoF6P→iminoE4Pはtransketolase_TktAによる)

close this sectionSequence

selected fasta
>3,4-dideoxy-4-amino-D-arabino-heptulosonate 7-phosphate synthase [RifH]
MKRQPDFAAHGRAVDRVLAGRLSAALARPAAQQPGWPDAERAAEVIELLHDAEPIVAPEE
TARLRARLASVARGEAFLLQGGDCAETFAGNTEPHLRANLGVLARMGEVLAEAAGLPVVK
IARMAGQFAKPRSRTVDALGLPVYRGDIVNSPECTVAARTPDPHRMLLAYAHAAGAMDLV
RKLCAEGLEEPLAAQVVLAGARRASSPGGRPFPDFGRPPRPAEIYVSHEMLLLDYERAVL
RTDSSGPEPRLSSGLAHFLWIGERPASSTAPTSTFAELLSNPIGLKLGPSCTPEEVVEYV
RRLDPHRTPGRLTLVSRMGHAQVRELLPPIVEKVAASGHEVIWQCDPMHGNTRTSGNGYK
TRHFGHVVAELAGFFAVHRQLGTHPGGIHVEVTGDDVTECLGGAAGIAEADLPDRYLTAC
DPRLNADQSVELAFAIAKMLR
selected fasta
>3,4-dideoxy-4-amino-D-arabino-heptulosonate 7-phosphate synthase [RifH]
GTGAAGCGGCAGCCGGACTTCGCGGCCCACGGCCGGGCGGTCGACCGGGTGCTGGCCGGC
CGGCTGAGCGCGGCGCTGGCCCGGCCGGCCGCGCAGCAGCCGGGCTGGCCGGACGCCGAG
CGGGCGGCCGAGGTGATCGAGCTGCTGCACGACGCCGAGCCGATCGTGGCGCCGGAGGAG
ACCGCACGGTTGCGCGCCCGGCTCGCGTCGGTCGCCCGCGGCGAGGCGTTCCTGCTGCAG
GGCGGGGACTGCGCGGAGACGTTCGCCGGCAACACCGAGCCGCACCTGCGGGCCAACCTC
GGGGTGCTGGCGCGGATGGGCGAAGTGCTGGCCGAGGCGGCCGGCCTGCCGGTGGTCAAG
ATCGCCCGGATGGCCGGGCAGTTCGCCAAGCCGCGGTCCCGGACCGTCGACGCGCTGGGC
CTGCCGGTGTACCGGGGCGACATCGTGAACTCCCCGGAGTGCACGGTCGCCGCCCGGACC
CCCGACCCGCACCGGATGCTGCTGGCATACGCCCACGCCGCCGGCGCGATGGACCTCGTC
CGCAAGCTCTGCGCCGAGGGCCTCGAAGAACCCCTGGCGGCGCAGGTGGTGCTCGCGGGC
GCCCGCCGCGCGTCGTCGCCCGGCGGGCGGCCGTTCCCGGACTTCGGCCGGCCACCGCGC
CCGGCCGAGATCTACGTCAGCCACGAAATGCTCCTGCTCGACTACGAGCGGGCCGTCCTG
CGGACGGATTCCAGCGGGCCGGAACCGCGGCTGTCCAGCGGGCTGGCGCACTTCCTGTGG
ATCGGCGAGCGCCCCGCCAGCTCGACGGCGCCCACATCGACGTTCGCGGAGCTGCTGTCC
AACCCGATCGGGCTGAAGCTGGGGCCGAGCTGCACCCCGGAGGAGGTCGTCGAGTACGTG
CGGCGGCTCGACCCGCACCGCACGCCGGGCCGGCTGACGCTGGTCAGCCGGATGGGGCAC
GCCCAGGTCCGGGAGCTCCTCCCGCCGATCGTGGAGAAGGTCGCCGCCTCCGGGCACGAG
GTGATCTGGCAGTGCGACCCGATGCACGGCAACACCCGGACCTCGGGCAACGGCTACAAG
ACCCGGCACTTCGGCCACGTCGTCGCCGAGCTGGCCGGCTTCTTCGCCGTGCACCGGCAG
CTCGGCACCCATCCCGGCGGCATCCACGTCGAGGTGACCGGCGACGACGTGACGGAATGC
CTCGGCGGGGCCGCGGGCATCGCGGAGGCCGACCTGCCCGACCGCTACCTGACCGCCTGC
GATCCGCGGCTCAACGCCGACCAGTCGGTCGAACTCGCGTTCGCGATCGCGAAGATGCTG
CGCTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002480 DAHP synthetase, class II (Family)
[24-190] 1.40000000000001e-57 PF01474 [222-437] 8.29999999999993e-92 PF01474
PF01474   DAHP_synth_2
SignalP
[1-27] 0.122 Signal
Eukaryota   
TMHMM No significant hit
Rifam_00280 Rifam_00280   Rifam_00300 Rifam_00300
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