Rifam_00400 Rifam_00400   Rifam_00420 Rifam_00420

Rifam_00410 : CDS information

close this sectionLocation

Organism
StrainS699
Entry nameRifamycin
Contig
Start / Stop / Direction83,827 / 82,562 / - [in whole cluster]
83,827 / 82,562 / - [in contig]
Locationcomplement(82562..83827) [in whole cluster]
complement(82562..83827) [in contig]
TypeCDS
Length1,266 bp (421 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productcytochrome P450
Product (GenBank)putative cytochrome P450 monooxygenase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF5
Reference
ACC
PmId
[9512878] Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699. (Chem Biol. , 1998)
[16079331] Identification of tailoring genes involved in the modification of the polyketide backbone of rifamycin B by Amycolatopsis mediterranei S699. (Microbiology. , 2005)
comment
[PMID: 9512878](1998)
ansamycin系抗生物質rifamycinの生合成gene clusterの報告。
Orf5(421aa): cytochrom P450 monooxygenase

---
[PMID: 16079331](2005)
cytochrome P450候補ORF4つ(ORF4, 5, 13, 16)のinactivation mutantを作成し、表現型を解析。

orf5 mutantはrifamycin B産生が抑制され、中間体であるrifamycin Wが蓄積した。また、cytochrome P450 monooxygenases inhibitorであるancymidolで処理したwild株の二次代謝産物をTLC, HPLC and ESI-MS analysesで解析し、やはりrifamycin Wを蓄積していることを確認。
よってRif-Orf5はrifamycin Wからの変換を担うcytochrome P450である。

この結果をふまえ、Orf5はrifamycin WのC12-C29二重結合を酸化的切断し、ketal構造を形成するための再構築反応を導くと提唱。

close this sectionSequence

selected fasta
>cytochrome P450 [putative cytochrome P450 monooxygenase]
MTTTAETSAEPVDLFSPEVVADPWGAHAAVREAPTLQIGGFMGGPPMYLAARYEDVRQVL
MDQRFQCNPLADSPAEDIRNGVFRHLDMPEELIPWLKNLINASDGEEHARLRKLVSYALT
AHRVGKFRPRVEELTAELLDKLEAAGGDGTPIDLVESFCYPLPVTVICELVGVDHEDRPQ
WRAWSDAMATLDGKRLPEEVRKTLAAARGMIERRRAEPKDDMVTALVEAQAKNPGIATDD
ELVGVLFSLVTAGHQTTTYLIGNSVLALHEHPDQLARLKADPSLWPQAVRELQRLGPIQF
GQPRFATEDIEVGGTVIPRGMPVVPLIMAANSDPRKFPEPEKLQIDRLAVGSESHLGFGK
GIHRCLGQHVAYLEAEVALRGLYTRFPDMTLAVPREEIPWILRPGFTRTKDLPVRLARPA
S
selected fasta
>cytochrome P450 [putative cytochrome P450 monooxygenase]
ATGACCACCACTGCCGAGACTTCGGCCGAACCGGTCGACCTGTTCTCCCCGGAAGTGGTC
GCCGACCCGTGGGGAGCGCACGCGGCGGTCCGCGAGGCGCCGACGCTGCAGATCGGCGGC
TTCATGGGCGGGCCGCCGATGTACCTGGCCGCCCGCTACGAAGACGTCCGCCAGGTCCTG
ATGGACCAGCGGTTCCAGTGCAACCCGCTGGCCGATTCCCCCGCCGAGGACATCCGCAAC
GGCGTCTTCCGGCACCTGGACATGCCGGAGGAGCTGATCCCCTGGCTGAAGAACCTCATC
AACGCCTCCGACGGCGAGGAGCACGCCCGGCTGCGCAAGCTGGTCTCCTACGCGCTCACC
GCGCACCGGGTCGGCAAGTTCCGCCCCCGGGTCGAAGAGCTCACCGCGGAGCTGCTCGAC
AAGCTGGAAGCGGCGGGCGGTGACGGCACGCCGATCGACCTCGTCGAGTCGTTCTGCTAC
CCGCTGCCGGTCACCGTGATCTGCGAGCTGGTCGGCGTCGACCACGAAGACCGGCCGCAG
TGGCGTGCGTGGAGCGACGCGATGGCCACCCTGGACGGCAAGCGGCTCCCGGAAGAAGTG
CGCAAGACCCTCGCCGCCGCCCGCGGGATGATCGAGCGGCGCCGCGCCGAGCCGAAGGAC
GACATGGTCACCGCGCTCGTCGAGGCGCAGGCCAAGAACCCCGGCATCGCCACCGACGAC
GAGCTGGTCGGCGTCCTGTTCAGCCTGGTCACCGCCGGGCACCAGACGACCACCTACCTG
ATCGGCAACTCGGTGCTGGCCCTGCACGAGCACCCCGACCAGCTCGCGCGGCTCAAGGCC
GACCCGTCGCTGTGGCCGCAGGCCGTGCGCGAACTGCAGCGCCTGGGCCCGATCCAGTTC
GGGCAGCCGCGGTTCGCCACCGAGGACATCGAGGTGGGCGGCACGGTGATCCCGCGCGGC
ATGCCCGTGGTGCCGCTGATCATGGCCGCCAACAGCGACCCGCGGAAGTTCCCCGAGCCG
GAGAAGCTGCAGATCGACCGGCTCGCCGTCGGCAGCGAGAGCCACCTCGGCTTCGGCAAG
GGCATCCACCGCTGCCTCGGCCAGCACGTCGCCTACCTGGAGGCGGAAGTGGCCCTGCGC
GGCCTGTACACCCGGTTCCCGGACATGACGCTGGCGGTGCCGCGCGAAGAGATCCCGTGG
ATCCTGCGGCCCGGCTTCACCCGGACCAAGGACCTCCCGGTGCGGCTCGCCCGGCCGGCG
TCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[12-418] 1.2e-95 SSF48264
SSF48264   Cytochrome P450
[12-417] 4.1e-101 G3DSA:1.10.630.10
G3DSA:1.10.630.10   no description
[287-386] 5.6e-17 PF00067
PF00067   p450
IPR002397 Cytochrome P450, B-class (Family)
[103-114] 1.6e-47 PR00359 [153-169] 1.6e-47 PR00359 [170-185] 1.6e-47 PR00359 [205-227] 1.6e-47 PR00359 [287-298] 1.6e-47 PR00359 [304-331] 1.6e-47 PR00359 [332-347] 1.6e-47 PR00359 [356-365] 1.6e-47 PR00359 [365-376] 1.6e-47 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[358-367] PS00086
PS00086   CYTOCHROME_P450
SignalP
[1-28] 0.145 Signal
Bacteria, Gram-negative   
[1-28] 0.067 Signal
Eukaryota   
[1-28] 0.149 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Rifam_00400 Rifam_00400   Rifam_00420 Rifam_00420
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