Rifam_00400 : CDS information

Location

Organism	Amycolatopsis mediterranei
Strain	S699
Entry name	Rifamycin
Contig	AF040570
Start / Stop / Direction	82,531 / 81,320 / - [in whole cluster] 82,531 / 81,320 / - [in contig]
Location	complement(81320..82531) [in whole cluster] complement(81320..82531) [in contig]
Type	CDS
Length	1,212 bp (403 aa)

Annotation

Category	5.1 general function
Product	putative cytochrome P450
Product (GenBank)	putative cytochrome P450 oxidoreductase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF4
Reference	ACC O52560 PmId [9512878] Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699. (Chem Biol. , 1998) [16079331] Identification of tailoring genes involved in the modification of the polyketide backbone of rifamycin B by Amycolatopsis mediterranei S699. (Microbiology. , 2005) comment [PMID: 9512878](1998) ansamycin系抗生物質rifamycinの生合成gene clusterの報告。 Orf4(403aa): cytochrom P450 monooxygenase --- [PMID: 16079331](2005) cytochrome P450候補ORF4つのinactivation mutantを作成し、表現型を解析。 orf4とorf13のmutantはrifamycin B産生に影響しなかった。
Related Reference	ACC B3XWK3 PmId [18640605] Increase in pladienolide D production rate using a Streptomyces strain overexpressing a cytochrome P450 gene. (J Biosci Bioeng. , 2008) comment 7th(B3XWK3) 51%, 1e-108 Streptomyces sp. A-1544_psmA Cytochrome P450 monooxygenase Pladienolide Bの16-hydroxylated derivativeであるpladienolide D産生を促進するための株Streptomyces bungoensis A-1544から分離したpladienolide B 16-hydroxylase PsmA。 PsmAの過剰発現で15倍のpladienolide Dを得た。 ferredoxin_psmBが一緒でないと変換率はぐっと低くなる。

Sequence

>putative cytochrome P450 [putative cytochrome P450 oxidoreductase]
MTETVAFPQDRTCPYQPAPGYEPLADRRPIAEVTLYDGRKAWAVTGHELARKLLCDPRIS
SDRTNPAWPMVSAAAAVEFTDVQQKVIKLLTALVGVDGPAHRARRKLVQAGFTVKRIDSL
RPKIQALVDRQIDEMIAEGAPVDLLKKFASPVPLIALCDLLGIPHEDQDYFEKKAHQILF
GPDAGGAYDELMAYLTKLIDKAEQNPGEEGFLQALLAERDPSSDVDHEEILQMFLIVLVT
GHDTTSSSIGLGAFTLLQHPERLAELRADPSLMPAAVDELTRFVVVPDGLQRVAADDIEV
DADTTIRKGDGVFFLFSLINRDEEAYEDGDRLDWHRPTFRDHLTYGFGTHQCAGMNHARA
IMEIAFRRLIDRLPGLRLAVPADEVRVKPGDAFQGLAELPVTW

>putative cytochrome P450 [putative cytochrome P450 oxidoreductase]
TTGACGGAGACCGTCGCCTTCCCGCAAGACCGCACCTGCCCGTACCAGCCGGCGCCGGGT
TACGAACCGCTCGCCGACCGGCGCCCGATCGCCGAGGTCACCCTCTACGACGGCCGCAAG
GCCTGGGCGGTCACCGGGCACGAACTGGCCCGCAAGCTGCTGTGCGACCCGCGCATCTCC
AGTGACCGGACCAATCCCGCGTGGCCGATGGTCAGCGCGGCCGCGGCCGTCGAGTTCACC
GACGTCCAGCAGAAGGTCATCAAGCTGCTCACCGCGCTGGTGGGCGTCGACGGCCCGGCC
CACCGGGCGCGGCGCAAGCTGGTGCAGGCGGGGTTCACGGTCAAGCGGATCGACTCCCTG
CGGCCGAAGATCCAGGCGCTCGTCGACCGGCAGATCGACGAGATGATCGCCGAAGGCGCT
CCGGTGGACCTGCTCAAGAAGTTCGCTTCGCCGGTGCCCCTGATCGCGTTGTGCGATCTG
CTCGGCATCCCGCACGAGGACCAGGACTACTTCGAGAAGAAGGCGCACCAGATCCTGTTC
GGGCCGGACGCCGGCGGGGCCTACGACGAGCTGATGGCCTACCTGACGAAGCTGATCGAC
AAGGCCGAGCAGAACCCGGGCGAAGAGGGGTTCCTGCAGGCCCTGCTCGCCGAGCGCGAC
CCGTCGAGCGACGTGGACCACGAGGAAATCCTGCAGATGTTCCTCATCGTGCTGGTGACC
GGGCACGACACGACGTCGAGCTCGATCGGGCTCGGTGCCTTCACGCTGCTGCAGCACCCG
GAACGGCTGGCCGAGCTGCGGGCGGACCCGTCGCTGATGCCCGCCGCGGTGGACGAGCTG
ACCCGGTTCGTGGTCGTCCCCGACGGCCTGCAGCGGGTGGCCGCGGACGACATCGAGGTC
GACGCGGACACCACCATCCGCAAGGGCGACGGTGTGTTCTTCCTGTTCTCGCTCATCAAC
CGGGACGAGGAAGCCTACGAGGACGGCGACCGGCTGGATTGGCACCGCCCGACGTTCCGC
GACCACCTGACCTACGGGTTCGGGACGCACCAGTGCGCGGGGATGAACCACGCGCGCGCC
ATCATGGAAATCGCTTTCCGGCGGCTGATCGACCGGCTGCCGGGATTGCGCCTGGCGGTC
CCGGCCGACGAAGTCCGGGTCAAGCCGGGGGACGCGTTCCAAGGACTGGCCGAGCTGCCC
GTCACTTGGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) G3DSA:1.10.630.10 no description PF00067 p450 SSF48264 Cytochrome P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450
SignalP	No significant hit
TMHMM	No significant hit

Rifam_00390 Rifam_00410