Rifam_00430 Rifam_00430   Rifam_00470 Rifam_00470

Rifam_00440 : CDS information

close this sectionLocation

Organism
StrainS699
Entry nameRifamycin
Contig
Start / Stop / Direction86,563 / 87,207 / + [in whole cluster]
86,563 / 87,207 / + [in contig]
Location86563..87207 [in whole cluster]
86563..87207 [in contig]
TypeCDS
Length645 bp (214 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative NDP-hexose epimerase
Product (GenBank)putative dNTP-hexose 3,5-epimerase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF8
Reference
ACC
PmId
[9512878] Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699. (Chem Biol. , 1998)
comment
ansamycin系抗生物質rifamycinの生合成gene clusterの報告。
Orf8(206aa): dNTP-hexose 3,5 epimerase

orf6-10はamino-deoxyhexose nucleotide形成をコードするgenesのようで、Orf8はD体→L体への異性化を触媒することが想定される。

しかしこれまでにA. mediterranei S699ではglycosylateされたrifamycinは報告されていないのでsilent geneかもしれない。これに一致して、orf6, orf9の不活化はrifamycin B産生に影響しない。

Streptomyces tolypophorusではglycosylated rifamycin derivativesであるtolypomycinsが分離されており、この菌はrifamycin Bも産生する。tolypomycinsのC4に付加する糖4-amino-2,3-dideoxy-L-rhamnoseの生合成後期段階には、 orf6-10によってコードされるような酵素を必要とする。
Related Reference
ACC
Q9S0P2
NITE
Aver_00150
PmId
[11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001)
comment
Blast 15th, id52%, 3e-45
Streptomyces avermitilis_aveBV(avrF)
dTDP-4-keto-6-deoxyhexose 3,5-epimerase
[Aver_00150]dTDP-4-keto-6-deoxyhexose 5-epimerase

Table1.
avrF/aveBV: dTDP-4-keto-6-deoxyglucose 3-epimerase　
(本文を読むと5-epimeraseの間違いと思われる。)

S.lividansでS.avermitilis avrBCDEFGHIを発現させて外因性aglyconeと反応させて産物解析。
delta-avrFな発現株では、C-13にD-olivoseが1つbeta-glycoside結合したavermectin A1a3を産生する。
avermectin A1a3を産生できるのはdelta-avrFのみ。
C-3での立体化学は最終的に3-O-methyltransferaseによって決定されるので、AvrF proteinは5-epimeraseであって3,5-epimeraseではない。

close this sectionSequence

selected fasta
>putative NDP-hexose epimerase [putative dNTP-hexose 3,5-epimerase]
MGSVSTRSMTVQPLAIDGAHVVTSQSFGDERGDFFEVYRQDVLTEALGYRPRFLQTNVST
SCRNVVRGVHGAMTPPGLAKFVTCLRGSLLDFVVDVRTGSPTFGQWDMIVLNQHGGTAVY
VEEGLGHAFVSLEDDTCVQYLLSDLYRPAEVLTVQPLDPEIGLPLNLPGEPILSERDAAA
PTLREAGELGLLPRYEECLALREARVRLEVSVAS
selected fasta
>putative NDP-hexose epimerase [putative dNTP-hexose 3,5-epimerase]
ATGGGTAGCGTCAGCACCCGCAGCATGACCGTTCAGCCGCTGGCCATCGACGGCGCACAC
GTGGTCACGTCCCAGTCGTTCGGCGACGAGCGCGGCGACTTCTTCGAGGTCTACCGCCAG
GACGTGCTCACCGAGGCGCTCGGCTACCGGCCGCGGTTCCTGCAAACGAACGTCTCGACG
TCGTGCCGCAACGTCGTGCGCGGCGTGCACGGGGCGATGACCCCGCCCGGCCTGGCGAAG
TTCGTGACGTGCCTGCGCGGTTCGCTGCTCGACTTCGTCGTCGACGTGCGGACCGGTTCC
CCGACGTTCGGCCAGTGGGACATGATCGTGCTCAACCAGCACGGCGGGACCGCGGTGTAC
GTCGAGGAAGGCCTCGGCCACGCGTTCGTGTCCCTCGAAGACGACACGTGCGTCCAGTAC
CTGCTGTCCGACCTCTACCGGCCGGCGGAGGTGCTCACCGTGCAGCCGCTGGACCCGGAA
ATCGGCCTCCCGCTGAACCTGCCGGGCGAGCCGATCCTGTCCGAGCGCGACGCGGCGGCG
CCGACGTTGCGGGAGGCCGGCGAGCTGGGCCTGCTGCCGCGGTACGAGGAGTGCCTCGCG
CTGCGGGAGGCGCGCGTGCGGCTCGAAGTGTCCGTGGCTTCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000888 dTDP-4-dehydrorhamnose 3,5-epimerase-related (Family)
[12-185] 3e-51 PF00908
PF00908   dTDP_sugar_isom
[14-186] 6e-98 PD001462
PD001462   O52564_AMYMD_O52564;
IPR011051 RmlC-like cupin domain (Domain)
[9-190] 7.8e-64 SSF51182
SSF51182   RmlC-like cupins
IPR014710 RmlC-like jelly roll fold (Domain)
[9-198] 1.6e-56 G3DSA:2.60.120.10
G3DSA:2.60.120.10   no description
SignalP No significant hit
TMHMM No significant hit
Rifam_00430 Rifam_00430   Rifam_00470 Rifam_00470
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