Rifam_00440 Rifam_00440   Rifam_00480 Rifam_00480

Rifam_00470 : CDS information

close this sectionLocation

Organism
StrainS699
Entry nameRifamycin
Contig
Start / Stop / Direction88,562 / 87,270 / - [in whole cluster]
88,562 / 87,270 / - [in contig]
Locationcomplement(87270..88562) [in whole cluster]
complement(87270..88562) [in contig]
TypeCDS
Length1,293 bp (430 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative aminotransferase
Product (GenBank)putative aminotransferase
Gene
Gene (GenBank)
EC number2.6.1.-
Keyword
Note
Note (GenBank)
  • ORF9
Reference
ACC
PmId
[9512878] Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699. (Chem Biol. , 1998)
[11278540] Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699. (J Biol Chem. , 2001)
[16079331] Identification of tailoring genes involved in the modification of the polyketide backbone of rifamycin B by Amycolatopsis mediterranei S699. (Microbiology. , 2005)
comment
[PMID: 9512878](1998)
ansamycin系抗生物質rifamycinの生合成gene clusterの報告。
Orf9(432aa): aminotransferase

orf6-10はamino-deoxyhexose nucleotide形成をコードするgenesのようで、Orf9はketo基のaminationを触媒し、aminosugarを形成することが想定される。

しかしこれまでにA. mediterranei S699ではglycosylateされたrifamycinは報告されていないのでsilent geneかもしれない。これに一致して、orf6, orf9の不活化はrifamycin B産生に影響しない。

Streptomyces tolypophorusではglycosylated rifamycin derivativesであるtolypomycinsが分離されており、この菌はrifamycin Bも産生する。tolypomycinsのC4に付加する糖4-amino-2,3-dideoxy-L-rhamnoseの生合成後期段階には、 orf6-10によってコードされるような酵素を必要とする。

---
[PMID: 11278540](2001)
[PMID: 16079331](2005)

orf9の不活化はrifamycin B産生に影響しないということが3論文全てでmutantを使って確認されている([PMID: 9512878]はunpublished data引用)。
Related Reference
ACC
Q6JE91
PmId
[15184572] Rhodococcus opacus expresses the xsc gene to utilize taurine as a carbon source or as a nitrogen source but not as a sulfur source. (Microbiology. , 2004)
comment
267th, 32%, 8e-38
Rhodococcus opacus (Nocardia opaca)_tpa
Taurine-pyruvate aminotransferase

Taurineを炭素源もしくは窒素源として利用する際に発現するORFを調べてin vitroで活性を確認している。
R.opacusのnative tpa(taurine-pyruvate aminotransferase), ald(alanine dehydrogenase), xsc(sulfoacetaldehyde acetyltransferase)

close this sectionSequence

selected fasta
>putative aminotransferase [putative aminotransferase]
MTVQKAPSQLLAAKLQIPPIPYSHAAGSWVYASDGTRYLDGASGIVNVNIGHAHPTVVAA
LRDQVGICTYASPGTFAPDLMEQLAAAAARAVHRPDDRVMFTPTGTHATESAIALARLVQ
RARGEEGRHKILTSTLGYHGNSAFVLALSGHRSRRPHEDDSFGLAPAFNPPYPGQHLGCP
FDACRVECVRAVRDAIVAAGPESVAAVLMEPVNGTTGGGYVPPAGYLRALREVCDEFGVL
VIHDEVLTGLGRCGLPLASHHTPGSAADIVTLSKGLGAGYVPLAATMLSPDLAEDIMTSG
KWLPLMGTMSATPLQGRAGLAVLSVLEELGALDRDEVRGAALGRLVADAVRGRQVVTDVR
GLGYFYGIELAPGTLGDVMRITRSRGLLLYPYVGFRPDRTGEGLLVAPRSTRPTAISASS
ARRWARHWTS
selected fasta
>putative aminotransferase [putative aminotransferase]
ATGACCGTGCAGAAAGCGCCGTCCCAGCTGCTGGCGGCCAAGCTGCAGATCCCCCCGATC
CCCTACAGCCACGCCGCCGGGTCCTGGGTGTACGCCTCCGACGGCACGCGCTACCTCGAC
GGCGCCAGCGGGATCGTCAACGTGAACATCGGCCACGCCCACCCCACGGTGGTCGCGGCC
CTGCGGGACCAGGTCGGGATCTGCACCTACGCCAGCCCCGGCACGTTCGCGCCCGACCTG
ATGGAGCAGCTGGCCGCGGCCGCCGCCCGGGCGGTGCACCGCCCGGACGACCGGGTGATG
TTCACCCCCACCGGCACGCACGCAACGGAATCCGCCATCGCGCTGGCCCGGCTGGTGCAG
CGGGCCCGCGGCGAAGAGGGCAGGCACAAGATCCTGACCTCGACGCTGGGCTACCACGGC
AACAGCGCGTTCGTGCTCGCCCTGTCGGGCCACCGGTCCCGGCGGCCGCACGAGGACGAC
AGCTTCGGCCTCGCTCCGGCGTTCAACCCGCCCTACCCCGGCCAGCACCTCGGCTGCCCG
TTCGACGCCTGCCGGGTCGAGTGCGTGCGCGCGGTGCGCGACGCGATCGTCGCGGCCGGC
CCGGAGAGCGTCGCCGCGGTGCTGATGGAGCCGGTCAACGGCACGACCGGCGGCGGGTAC
GTCCCGCCGGCGGGCTACCTGCGGGCGCTGCGCGAGGTCTGCGACGAGTTCGGCGTCCTC
GTCATCCACGACGAGGTCCTCACCGGGCTGGGCCGCTGCGGGCTCCCGCTGGCCTCGCAC
CACACGCCGGGTTCGGCGGCGGACATCGTCACGCTGTCGAAGGGCCTCGGCGCGGGGTAC
GTCCCGCTGGCGGCCACGATGCTCTCGCCGGACCTGGCCGAGGACATCATGACCAGCGGC
AAGTGGCTGCCGCTGATGGGCACGATGTCGGCGACCCCGCTGCAGGGCCGGGCCGGGCTG
GCGGTGCTTTCGGTGCTGGAGGAGCTCGGCGCCCTCGACCGCGACGAAGTCCGCGGCGCG
GCCCTCGGCCGGCTCGTGGCCGACGCCGTGCGCGGCCGGCAGGTGGTGACGGACGTCCGC
GGGCTCGGGTACTTCTACGGCATCGAGCTGGCTCCCGGCACGCTCGGCGACGTCATGCGG
ATCACCCGGAGCCGGGGCCTGCTGCTGTACCCGTACGTCGGCTTCCGCCCCGACCGCACG
GGCGAGGGCCTGCTGGTGGCCCCCCGCTCAACGCGACCGACGGCGATCTCGGCTTCCTCG
GCGAGACGCTGGGCGCGGCACTGGACCAGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005814 Aminotransferase class-III (Family)
[241-279] PS00600
PS00600   AA_TRANSFER_CLASS_3
[22-371] 6e-65 PF00202
PF00202   Aminotran_3
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
[55-328] 2.8e-61 G3DSA:3.40.640.10
G3DSA:3.40.640.10   no description
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
[330-391] 1e-16 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   no description
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
[18-429] 6.8e-86 SSF53383
SSF53383   PLP-dependent transferases
SignalP
[1-25] 0.459 Signal
Bacteria, Gram-negative   
[1-25] 0.494 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Rifam_00440 Rifam_00440   Rifam_00480 Rifam_00480
Page top