Rifam_00480 : CDS information

Location

Organism	Amycolatopsis mediterranei
Strain	S699
Entry name	Rifamycin
Contig	AF040570
Start / Stop / Direction	89,579 / 88,587 / - [in whole cluster] 89,579 / 88,587 / - [in contig]
Location	complement(88587..89579) [in whole cluster] complement(88587..89579) [in contig]
Type	CDS
Length	993 bp (330 aa)

Annotation

Category	5.1 general function
Product	putative NDP-hexose C-3 ketoreductase
Product (GenBank)	putative dNTP-hexose 3-ketoreductase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF10
Reference	ACC O52566 PmId [9512878] Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699. (Chem Biol. , 1998) [16079331] Identification of tailoring genes involved in the modification of the polyketide backbone of rifamycin B by Amycolatopsis mediterranei S699. (Microbiology. , 2005) comment [PMID: 9512878](1998) ansamycin系抗生物質rifamycinの生合成gene clusterの報告。 Orf10(330aa): oxidoreductase orf6-10はamino-deoxyhexose nucleotide形成をコードするgenesのようで、Orf10はRifLに似たoxidoreductaseをコードする。しかしこれまでにA. mediterranei S699ではglycosylateされたrifamycinは報告されていないのでsilent geneかもしれない。これに一致して、orf6, orf9の不活化はrifamycin B産生に影響しない。 Streptomyces tolypophorusではglycosylated rifamycin derivativesであるtolypomycinsが分離されており、この菌はrifamycin Bも産生する。tolypomycinsのC4に付加する糖4-amino-2,3-dideoxy-L-rhamnoseの生合成後期段階には、 orf6-10によってコードされるような酵素を必要とする。 --- [PMID: 16079331](2005) orf10の不活化mutant作成あり。rifamycin B産生に影響しない。
Related Reference	ACC Q9ALN5 NITE Spino_00080 PmId [18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008) comment Blast 14th, id51%, 3e-79 Saccharopolyspora spinosa_spnN Probable NDP-hexose-3-ketoreductase -- NAD(P)H-dependent dTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductase_SpnN dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), TylX3(SpnNの1つ前の反応を担うSpnOのhomologue), SpnN, and NADPHをインキュベーションしてできた産物がdTDP-4-keto-2,6-dideoxy-D-glucoseであることをHPLC chromatogram、1H NMR spectrumなどで確認している。NADPHはNADHの2倍速く消費されるので、おそらくNADPH-dependentである。

Sequence

>putative NDP-hexose C-3 ketoreductase [putative dNTP-hexose 3-ketoreductase]
MAPLRIGIMGCASIAVRKVLPAMAASPRTEITAIASRNPGKAAEIAHAYGCRAVEGYSAL
LDLDEVEAVYIPLPNAAHAGWIELALTAGKHVLAEKPLTTSVSRTCELIAAAGAAGLVLM
ENVMFLHHSQHTAVRDRLAGGAIGELRAFHAAFAVPARPAGDIRHRAELGGGALLDTGVY
PVRAAMSFLGSEVDVVSAVLTRRHGRPVDSAGQALLCTADGVSASATFGIDHAYRSGYEL
WGSEGRIVLDHAFTPPADHLPVVRLERRSGVDEIPLPPDDQVANTLTAFAAAVRSGRLPD
NDDVARQAVLVDDIRTKARLYVDHAPEPVG

>putative NDP-hexose C-3 ketoreductase [putative dNTP-hexose 3-ketoreductase]
GTGGCCCCACTGCGCATCGGGATCATGGGCTGCGCGAGCATCGCCGTCCGGAAGGTGCTT
CCCGCCATGGCCGCCTCGCCCCGCACGGAAATCACGGCGATCGCCAGCCGGAACCCCGGC
AAGGCCGCGGAAATCGCGCACGCCTACGGGTGCCGCGCGGTCGAGGGCTATTCGGCGCTG
CTGGACCTCGACGAGGTCGAGGCCGTCTACATCCCCCTGCCGAACGCCGCGCACGCGGGC
TGGATCGAGCTGGCGCTGACCGCCGGCAAGCACGTGCTGGCCGAGAAACCGTTGACCACC
AGCGTTTCCCGAACCTGTGAGCTGATCGCCGCGGCCGGTGCGGCCGGGCTCGTGCTGATG
GAGAACGTCATGTTCCTCCACCACAGCCAGCACACGGCGGTCCGGGACCGGCTGGCCGGC
GGCGCGATCGGCGAGCTGCGGGCGTTCCACGCCGCCTTCGCCGTGCCGGCCCGCCCAGCC
GGCGACATCCGCCACCGCGCCGAGCTCGGCGGCGGTGCGCTGCTGGACACCGGCGTCTAC
CCGGTGCGCGCGGCGATGTCCTTCCTGGGCAGCGAAGTCGACGTCGTTTCGGCGGTGCTC
ACCCGGCGCCACGGCCGGCCCGTCGACTCCGCCGGGCAGGCGCTGCTGTGCACCGCCGAC
GGGGTGAGCGCGTCCGCGACCTTCGGGATCGACCACGCCTACCGCTCCGGATACGAACTT
TGGGGCAGCGAAGGGCGGATCGTCCTCGACCACGCCTTCACCCCGCCCGCCGACCACCTC
CCCGTGGTCCGCCTCGAACGGCGTTCGGGCGTCGACGAAATCCCGCTGCCGCCCGACGAC
CAGGTGGCGAACACGCTCACCGCCTTCGCCGCGGCCGTCCGTTCGGGACGGCTGCCCGAC
AACGACGACGTGGCCCGCCAAGCCGTGCTCGTCGACGACATCCGCACCAAGGCGAGGCTT
TACGTCGATCACGCCCCCGAGCCGGTGGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000683 Oxidoreductase, N-terminal (Domain) PF01408 GFO_IDH_MocA IPR004104 Oxidoreductase, C-terminal (Domain) PF02894 GFO_IDH_MocA_C IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	Eukaryota Bacteria, Gram-negative Bacteria, Gram-positive
TMHMM	No significant hit

Rifam_00470 Rifam_00490