Rifam_00530 : CDS information

Location

Organism	Amycolatopsis mediterranei
Strain	S699
Entry name	Rifamycin
Contig	AF040570
Start / Stop / Direction	96,037 / 94,826 / - [in whole cluster] 96,037 / 94,826 / - [in contig]
Location	complement(94826..96037) [in whole cluster] complement(94826..96037) [in contig]
Type	CDS
Length	1,212 bp (403 aa)

Annotation

Category	3.4 other modification
Product	C-25 O-acetyltransferase
Product (GenBank)	putative polyketide associated protein
Gene
Gene (GenBank)
EC number	2.3.1.-
Keyword
Note
Note (GenBank)	ORF20
Reference	ACC Q9AE01 PmId [16079331] Identification of tailoring genes involved in the modification of the polyketide backbone of rifamycin B by Amycolatopsis mediterranei S699. (Microbiology. , 2005) [15791687] A homologue of the Mycobacterium tuberculosis PapA5 protein, rif-orf20, is an acetyltransferase involved in the biosynthesis of antitubercular drug rifamycin B by Amycolatopsis mediterranei S699. (Chembiochem. , 2005) comment [PMID: 16079331](2005) PKS後修飾に関するgenesの報告。 --- [PMID: 15791687](2005) abstract Rif-Orf20はM. tuberculosis PapA5 proteinのhomologueで、DMDARSV→DMRSVのacetylationを担うことがわかっている。 DMDARSV: 27-O-demethyl-25-O-desacetylrifamycin SV DMRSV: 27-O-demethylrifamycin SV

Sequence

>C-25 O-acetyltransferase [putative polyketide associated protein]
MTRAAVRRALAPSESIHAVKEAYIGYTVRAEGRLDPEALTTAFEAVTRAYPQLSACVEFT
GEGAVLAESDARPEIRFADGDPGRPLEGLDLDQSRSLSALNVVRDGDEASVCLAIQHSVA
DAHHATAILSALWSCYTDVIEGVPLDLGRHPYPRSLEDLLAERGIHASVPAAPSVPPAAP
PPASRPDPVIRHVVQHRLTEAETTALAELGHREKVTINGLVSGAVLLAEAEIRDLPLTDL
VYRYSVNLRSRLTPPVGATEGTNVLDGVGFKVTDGIAHDAVAIGRAIGEQLRAGLADGSV
QRSILDMFARPAAAKPWDPKLALAVVSIMNWGVAPPMRTPDGLRLTNLHSASRMRETLAL
GGYVMSSFGGRVGLDVGWPEGDPSLPQRLDCLRERLARLTRDL

>C-25 O-acetyltransferase [putative polyketide associated protein]
GTGACGCGAGCGGCTGTCCGGCGCGCGCTGGCGCCCAGCGAGAGCATCCACGCCGTGAAA
GAGGCCTACATCGGGTACACCGTGCGTGCCGAGGGCCGGCTCGACCCCGAGGCGCTGACG
ACCGCGTTCGAAGCGGTCACTCGCGCCTACCCCCAGCTTTCGGCCTGCGTGGAGTTCACC
GGCGAGGGCGCGGTGCTCGCCGAGTCCGACGCGCGGCCGGAGATCCGGTTCGCCGACGGC
GATCCCGGCCGGCCACTCGAGGGATTGGACCTCGACCAGAGCCGTTCGCTGAGCGCGCTC
AACGTCGTCCGCGACGGCGACGAAGCCAGTGTCTGCCTGGCGATCCAGCACAGTGTCGCC
GACGCCCACCACGCCACCGCGATCCTCTCGGCGCTCTGGTCCTGCTACACCGACGTCATC
GAGGGCGTGCCGCTCGACCTGGGCCGGCACCCGTACCCGCGGTCGCTGGAGGACCTGCTG
GCCGAACGCGGCATCCACGCGAGCGTCCCGGCGGCGCCCTCGGTCCCACCGGCGGCACCG
CCGCCCGCGTCCCGGCCGGACCCCGTCATCCGGCACGTCGTCCAGCACCGGCTGACCGAA
GCCGAAACGACGGCGCTGGCGGAACTCGGCCACCGGGAGAAGGTGACGATCAACGGCCTG
GTGTCGGGCGCGGTCCTGCTCGCCGAGGCCGAGATCCGTGACCTCCCGCTGACCGACCTC
GTCTACCGGTACTCGGTGAACCTGCGCAGCAGGCTGACGCCCCCGGTCGGCGCGACCGAG
GGCACGAACGTGCTGGACGGGGTCGGGTTCAAGGTGACGGACGGGATCGCGCACGACGCC
GTCGCGATCGGCCGGGCCATCGGCGAGCAGCTGCGGGCCGGCCTCGCCGACGGGTCCGTG
CAGCGCAGCATCCTGGACATGTTCGCCCGGCCCGCCGCCGCGAAACCGTGGGACCCGAAG
CTCGCCCTCGCCGTGGTCAGCATCATGAACTGGGGCGTCGCCCCGCCGATGCGCACGCCG
GACGGCCTGCGGCTGACCAACCTGCACTCGGCGTCGCGGATGCGGGAAACCCTCGCGCTC
GGCGGTTACGTGATGAGCAGCTTCGGCGGCCGGGTGGGCCTCGACGTGGGCTGGCCCGAG
GGCGATCCTTCGCTGCCGCAGCGGCTGGACTGCCTGCGCGAACGGCTGGCCCGCCTGACT
CGTGACCTGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Rifam_00520 Rifam_00540