Rubra_00160 : CDS information

Location

Organism	Streptomyces achromogenes var. rubradiris
Strain	NRRL 3061 (=NBRC 14000)
Entry name	Rubradirin
Contig	AJ871581
Start / Stop / Direction	32,153 / 32,818 / + [in whole cluster] 32,153 / 32,818 / + [in contig]
Location	32153..32818 [in whole cluster] 32153..32818 [in contig]
Type	CDS
Length	666 bp (221 aa)

Annotation

Category	2.2 modification addition of starter unit
Product	putative hydrolase
Product (GenBank)	putative phosphatase
Gene
Gene (GenBank)	rubM
EC number
Keyword	AHBA
Note
Note (GenBank)
Reference	ACC Q2PC74 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) comment Rubradirin生合成clusterの報告。配列比較からのProposed function RubM(221aa): Phosphatase rifamycin産生株で見つかっているAHBA biosynthetic genes, rifK, L, M, N, G, H, and Jの産物と高度な相同性を示す（rubNを除く）6つの遺伝子がrubradirin生合成gene clusterにある。 rubK, rubL, rubM, and rubNはsingle operon。
Related Reference	ACC O52553 NITE Rifam_00330 PmId [11278540] Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699. (J Biol Chem. , 2001) [12207505] Characterization of the early stage aminoshikimate pathway in the formation of 3-amino-5-hydroxybenzoic acid: the RifN protein specifically converts kanosamine into kanosamine 6-phosphate. (J Am Chem Soc. , 2002) comment 7th, 70%, 6e-69 Amycolatopsis mediterranei_rifM RifM [Rifam_00330]putative hydrolase --- [PMID: 11278540](2001) rifamycinと関連ansamycinsのPKS starter 3-amino-5-hydroxybenzoic acid (AHBA)の生合成経路について調査。関連遺伝子の不活化、異種性発現、中間体を基質とした反応確認など。 RifM: phosphatase rifMは、PKSのstarter unitであるAHBAの合成に必要十分な7genes(rifG, H, J, K, L, M, N)のひとつだが、rifH, G, Jと異なりshikimate pathway関連遺伝子には似ていない。 rifM mutantの実験から、AHBA生合成の早期段階で作用し、aminoDAHP形成か、それより前で関与することが示唆されている。 --- [PMID: 12207505](2002) A.mediterraneiのrifN mutantは、rifLとrifMのmutant両方を相補してrifamycin B産生を回復することができたので、RifLとRifMは経路においてRifNより前で機能しなくてはいけない。 UDP-kanosamine→kanosamineへ触媒するのがRifMであるというschemaあるが、詳細な根拠なし。
Family/Domain	FD IPR006439 comment [IPR006439] HAD hydrolase, subfamily IA (Family) Function : hydrolase activity IPR006402, IPR005833のsignatureは現在 IPR006439 に統合されている。

Sequence

>putative hydrolase [putative phosphatase]
MASVPVRSVVIFDLDGVLVDSHEVMGRAFAAAYAEVVGAGPAPFAEYQRHQGLYFPDIMR
RMRLPLEMEEPFVRESYRLADQVPVADGVADLLQTLRTRGFRLAVATGKAGPRARSLLAT
LGLIQYFDHVVGSDEVANAKPAPDIVRRALSLLDAEPDAALMVGDAPADIRSARSAGVTA
VAATWATVDEDGLMAAGPDLVVHSPGELLPACPPVPAPGRR

>putative hydrolase [putative phosphatase]
ATGGCGAGCGTACCGGTGCGCAGTGTGGTGATCTTCGACCTCGACGGCGTCCTGGTGGAC
AGTCATGAGGTGATGGGCCGGGCCTTCGCCGCGGCGTACGCGGAAGTGGTCGGCGCCGGA
CCGGCGCCGTTCGCCGAATACCAGCGCCATCAGGGCCTGTATTTCCCGGATATCATGCGC
CGGATGCGCCTCCCGCTGGAGATGGAGGAGCCGTTCGTCCGGGAGAGCTACCGGCTCGCG
GACCAGGTGCCCGTGGCGGACGGGGTGGCCGATCTGCTCCAGACGCTCCGGACGCGTGGG
TTCCGGCTCGCCGTGGCCACCGGGAAGGCAGGGCCGCGGGCTCGCTCCCTGCTGGCCACG
CTCGGCCTGATCCAGTACTTCGACCACGTAGTGGGCTCGGACGAGGTCGCCAACGCCAAA
CCCGCCCCGGACATCGTCAGGCGCGCGCTCAGCCTGCTCGACGCGGAGCCGGACGCGGCC
CTCATGGTCGGCGATGCCCCGGCCGACATCCGCAGCGCCCGAAGCGCCGGGGTGACCGCC
GTAGCGGCGACCTGGGCGACCGTGGACGAGGACGGCCTGATGGCTGCCGGTCCGGATCTG
GTCGTCCACTCCCCCGGTGAACTGCTGCCGGCGTGCCCGCCGGTCCCGGCTCCGGGCCGG
CGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005833 Haloacid dehalogenase/epoxide hydrolase (Family) PR00413 HADHALOGNASE IPR006351 3-amino-5-hydroxybenzoic acid synthesis-related (Family) TIGR01454 AHBA_synth_RP IPR006402 HAD-superfamily hydrolase, subfamily IA, variant 3 (Family) TIGR01509 HAD-SF-IA-v3 IPR006439 HAD-superfamily hydrolase, subfamily IA, variant 1 (Family) TIGR01549 HAD-SF-IA-v1 IPR023214 HAD-like domain (Domain) SSF56784 HAD-like_dom G3DSA:3.40.50.1000 HAD-like_dom
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

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