Rubra_00160 Rubra_00160   Rubra_00180 Rubra_00180

Rubra_00170 : CDS information

close this sectionLocation

Organism
StrainNRRL 3061 (=NBRC 14000)
Entry nameRubradirin
Contig
Start / Stop / Direction32,815 / 33,738 / + [in whole cluster]
32,815 / 33,738 / + [in contig]
Location32815..33738 [in whole cluster]
32815..33738 [in contig]
TypeCDS
Length924 bp (307 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.2 modification addition of starter unit
Productputative kanosamine kinase
Product (GenBank)putative kinase
Gene
Gene (GenBank)rubN
EC number
Keyword
  • AHBA
Note
Note (GenBank)
Reference
ACC
PmId
[18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008)
comment
Rubradirin生合成clusterの報告。

配列比較からのProposed function
RubN(307aa): Kinase

rifamycin産生株で見つかっているAHBA biosynthetic genes, rifK, L, M, N, G, H, and Jの産物と高度な相同性を示す（rubNを除く）6つの遺伝子がrubradirin生合成gene clusterにある。
rubK, rubL, rubM, and rubNはsingle operon。

--
rubN以外がAHBA合成rif genesに高い相同性があると言われているが、RubN vs. RifNも他のものに比べれば劣るがかなり有意に相同性がある(id49%)。
Related Reference
ACC
O52554
NITE
Rifam_00340
PmId
[11278540] Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699. (J Biol Chem. , 2001)
[12207505] Characterization of the early stage aminoshikimate pathway in the formation of 3-amino-5-hydroxybenzoic acid: the RifN protein specifically converts kanosamine into kanosamine 6-phosphate. (J Am Chem Soc. , 2002)
comment
17th, 49%, 4e-35
Amycolatopsis mediterranei_rifN
RifN
[Rifam_00340]kanosamine kinase

---
[PMID: 11278540](2001)
rifamycinと関連ansamycinsのPKS starter 3-amino-5-hydroxybenzoic acid (AHBA)の生合成経路について調査。関連遺伝子の不活化、異種性発現、中間体を基質とした反応確認など。

どちらもRifN: kinase

rifNは、PKSのstarter unitであるAHBAの合成に必要十分な7genes(rifG, H, J, K, L, M, N)のひとつだが、rifH, G, Jと異なりshikimate pathway関連遺伝子には似ていない。

rifN mutantの実験から、AHBA生合成の早期段階で作用し、aminoDAHP形成か、それより前で関与することが示唆されている。

---
[PMID: 12207505](2002)
rifNについての実験論文。

E.coliでHis6 fusion proteinとして過剰発現、精製したRifNの基質特異性を様々な糖で確認。kanosamineのみが反応し、この反応生成物をNMRなどで解析したところ、kanosamine 6-phosphateと同定された。

よって、RifNはkanosamine kinase
kanosamine → kanosamine 6-phosphate

close this sectionSequence

selected fasta
>putative kanosamine kinase [putative kinase]
MTGRTAWPGSWLGIDFGGTKVALRAETDGGEVSEHTFRWRGRGLESDLSQLATELARFRR
RIPQGYTGIGVAMPANGGGGRAGHDLARTAPEWTGLDLRRTFRSHFGDTPVRWADDGYAG
PLGKRGRWAATKLLYVGVGTGVGGGLLAGGTLWPGPDRGSFELGHVVTDVDGPICSCGRR
GCLQATASGPATLARAAGMRGVPVGYDDLRQGLVADEEWAVQAVDRTCHRLAVAISGVRE
LLHPGTVVVGGGFAAGLPVFAGLVAHHLAALARPGVPAPVVRASVLGPLSTLHGAVALAR
LPGPDPL
selected fasta
>putative kanosamine kinase [putative kinase]
GTGACGGGGCGGACGGCATGGCCGGGAAGCTGGCTCGGCATCGATTTCGGTGGCACCAAG
GTGGCGCTGCGGGCGGAGACCGACGGCGGAGAAGTCAGCGAGCACACCTTCCGATGGCGC
GGGCGCGGGCTGGAGTCCGACCTGAGCCAGCTCGCGACCGAACTCGCCCGGTTCCGGCGG
CGAATCCCGCAGGGCTACACCGGAATCGGAGTCGCGATGCCGGCCAACGGTGGGGGCGGA
CGGGCTGGTCACGACCTGGCCCGAACCGCCCCCGAGTGGACCGGCCTTGATCTGCGGCGG
ACGTTCCGCTCGCACTTCGGGGACACCCCGGTCCGCTGGGCCGACGACGGGTATGCTGGG
CCCCTGGGCAAGCGCGGGCGCTGGGCTGCGACGAAGCTGCTCTATGTAGGGGTGGGAACG
GGCGTGGGCGGTGGGCTGCTCGCGGGCGGCACGCTGTGGCCCGGACCGGATCGCGGTTCC
TTCGAGCTCGGTCACGTGGTGACCGACGTGGACGGCCCGATCTGCTCGTGCGGGCGCCGC
GGGTGCCTTCAGGCCACCGCGTCCGGACCCGCGACGTTGGCGCGAGCGGCCGGGATGCGG
GGGGTGCCGGTCGGGTACGACGACCTCCGCCAGGGCCTGGTGGCGGACGAGGAGTGGGCG
GTCCAGGCCGTGGACCGCACCTGCCACCGGCTCGCGGTGGCCATCAGCGGGGTGCGAGAA
CTCCTGCACCCCGGCACGGTCGTGGTGGGGGGCGGCTTCGCCGCCGGACTGCCCGTGTTC
GCCGGCCTGGTCGCGCACCATCTGGCCGCGCTGGCCCGGCCGGGCGTGCCGGCACCGGTG
GTGCGGGCGTCTGTGCTGGGCCCGCTGTCCACGCTGCACGGCGCGGTCGCGCTGGCCCGC
CTGCCCGGACCGGATCCGCTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000600 ROK (Family)
[13-191] 3.8e-17 PF00480
PF00480   ROK
SignalP No significant hit
TMHMM
[133-155] Transmembrane (i-o) [241-263] Transmembrane (o-i)
Transmembrane 2   
Rubra_00160 Rubra_00160   Rubra_00180 Rubra_00180
Page top