Rubra_00280 : CDS information

Location

Organism	Streptomyces achromogenes var. rubradiris
Strain	NRRL 3061 (=NBRC 14000)
Entry name	Rubradirin
Contig	AJ871581
Start / Stop / Direction	47,128 / 48,414 / + [in whole cluster] 47,128 / 48,414 / + [in contig]
Location	47128..48414 [in whole cluster] 47128..48414 [in contig]
Type	CDS
Length	1,287 bp (428 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	hypothetical protein
Gene
Gene (GenBank)	rubF3
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q2PC62 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) comment Rubradirin生合成clusterの報告。配列比較からのProposed function RubF3(428aa): Rif20 rubF2はalcohol dehydrogenaseに、rubF3はNRPSのadenylation domainに相同性を示す。 RubF2 and RubF3はたぶんRubFとオペロンなので、完全に伸長されたpolyketide鎖のC末は、RubFで保存されているCys-73 residueに付着され(Payton et al. 2001)、RubF3によって移動、adenyl化され、RubF2によってcyclic amineに還元されると考えられる。

Sequence

>hypothetical protein [hypothetical protein]
MRRRIARSERMYVSEIRTQALQICVVRGGVDAGILDAAFTAVLAEAPGLRSRLEKDGDDY
YLSLLEPSELPRLHVRRNEPGARLEEYNKPLHLGGPLARAVLLTGTDEDVVMFGVDHAIS
DGRSATAFCNRIWQRYIDIQSGGYSAPKPQQQQWPAPLEDWLPPRSDAELDAYVQERLER
AEGAPVASLPFRAAGSLPPVPAESLMTSRRLRLTESQTTGLLAFAKHTGVSVNGLVAAAL
LAAVRAGLPEELADHRLSSFCTVDLRNQFAPGLGHEAMVPMVSWYRDLLHVPAGADLVKL
GHLFSTELRAGIERGDAAMEMQALDRLLPHPQLWPTSLVLTNVGRIDGPPSPKGLKIVDM
TKFPLSSRWDPERGAGPLLASPATIYGRFSIEMPYSTQCFTTAQMDSIHDHVRDSLLACA
DRAPGSRR

>hypothetical protein [hypothetical protein]
ATGCGCCGACGAATTGCCCGGTCGGAACGGATGTACGTCAGCGAGATCAGGACGCAGGCG
CTGCAGATCTGCGTGGTGCGCGGCGGCGTCGACGCCGGGATTCTGGACGCGGCCTTCACC
GCTGTCCTCGCGGAGGCGCCAGGTCTGCGCAGCCGTCTGGAGAAGGACGGCGACGACTAC
TACCTCAGCCTGCTGGAGCCGAGCGAGCTGCCGCGTCTGCACGTCCGGCGCAACGAGCCC
GGTGCGCGGCTTGAGGAGTACAACAAGCCGTTGCACCTCGGTGGCCCGCTCGCGCGTGCC
GTCCTGCTGACCGGCACCGACGAGGACGTCGTGATGTTCGGCGTCGACCACGCGATCTCC
GACGGCCGCAGCGCGACCGCGTTCTGCAACCGGATCTGGCAGCGTTACATCGACATCCAG
TCCGGCGGCTACTCGGCCCCAAAGCCGCAACAGCAGCAGTGGCCGGCGCCGCTCGAGGAC
TGGCTGCCGCCGCGCAGCGATGCCGAGCTCGACGCCTACGTCCAGGAGCGGCTCGAGCGG
GCCGAGGGGGCACCGGTGGCGTCTTTGCCGTTCCGGGCGGCCGGGTCCCTCCCGCCGGTG
CCGGCGGAGAGTCTGATGACCTCGCGCAGGCTCCGACTGACCGAATCGCAGACCACTGGT
CTGCTGGCCTTCGCCAAGCACACCGGAGTATCGGTGAACGGGTTGGTCGCCGCGGCACTG
TTGGCGGCCGTGCGCGCCGGCCTGCCCGAGGAACTGGCCGACCACCGGCTGTCCAGCTTC
TGCACGGTCGACCTGCGCAACCAGTTCGCGCCGGGACTCGGCCACGAGGCGATGGTGCCC
ATGGTCTCCTGGTACCGCGATCTGCTCCACGTGCCCGCCGGCGCCGACCTGGTCAAGCTC
GGCCACCTGTTCAGCACCGAGCTGCGCGCCGGCATCGAGCGGGGTGACGCGGCGATGGAG
ATGCAGGCACTGGACCGGTTGCTCCCCCACCCCCAGCTCTGGCCGACCAGCCTGGTGCTC
ACCAACGTCGGAAGAATCGACGGGCCGCCGTCGCCGAAGGGGCTGAAGATCGTCGACATG
ACCAAGTTCCCGCTGTCCAGCCGGTGGGACCCGGAGCGCGGTGCGGGCCCGTTGCTCGCG
TCGCCGGCAACGATCTACGGCCGGTTCAGCATCGAGATGCCGTACAGCACGCAGTGCTTC
ACGACCGCGCAGATGGACTCGATCCACGACCACGTCCGCGATTCGCTGCTGGCATGCGCT
GATCGGGCCCCCGGTTCACGGCGCTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001242 Condensation domain (Domain) PF00668 Condensation
SignalP	No significant hit
TMHMM	No significant hit

Rubra_00270 Rubra_00290