Rubra_00290 : CDS information

Location

Organism	Streptomyces achromogenes var. rubradiris
Strain	NRRL 3061 (=NBRC 14000)
Entry name	Rubradirin
Contig	AJ871581
Start / Stop / Direction	49,686 / 48,466 / - [in whole cluster] 49,686 / 48,466 / - [in contig]
Location	complement(48466..49686) [in whole cluster] complement(48466..49686) [in contig]
Type	CDS
Length	1,221 bp (406 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	putative cytochrome P450
Gene
Gene (GenBank)	rubF4
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q2PC61 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) comment Rubradirin生合成clusterの報告。配列比較からのProposed function RubF4(406aa): Cytochrome P450 rubransarolのmorpholine環形成のための様式は不明なままだが、rubF4 to rubF7 and rubP3 genesがこの過程で役割を果たすかもしれない。
Related Reference	ACC A6YRR3 PmId [19376895] Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgenone to isonuatigenone in Streptomyces virginiae IBL-14. (Appl Environ Microbiol. , 2009) comment 31st, id38%, 4e-57 Streptomyces virginiae Cytochrome P450 monooxygenase 105 cytochrome P450 monooxygenase, FcpCを同定した文献。 FcpCがdiosgenoneのC-25のhydroxylationを担い、isonuatigenoneに変換する機能を持つことがin vitroで示されている。この論文で同定されたfcpC(1191nt) and fcpD(243nt)遺伝子配列のGenBank accession numberはEF646279であると記載あり。よってこのentry=FcpCで合っている。

Sequence

>putative cytochrome P450 [putative cytochrome P450]
MTEGIPEPISYLRHWDRTEPFDPPAVLGELVRTRPLARMVYPDGHVGWLATSHELAREVL
GSQAFSHRFHTTHAPVTKKGEIFPSVPIVPGGFIHMDPPEHTRYRNLLSSEFSARATAAY
APRVAELAAAQLAQVRQHGSPVDLLPTYVRPLSLRVLCEVLGVPFTGSPVLSALSDSATD
DEVSLEAEREAGTRAYHYLLDLVGKARVEPGENVTGRLAADPELTDREITNMLLIVFAAG
FASCEGALASSLLALLHHRDQLDRLRSAARSGGADGIEKMVEELLRYTSVNQFQIFRTAL
SDVELGGELVREGETVTVALPAANRDPARFGCPDGLDVSQDASAHLAFGYGVHACVGQRL
ARLVLAEGLTALVLGFPGLALDVPLREVPVRLKTPVLGVLRLPVRY

>putative cytochrome P450 [putative cytochrome P450]
ATGACTGAGGGAATCCCGGAACCGATCTCCTATCTGCGGCACTGGGACCGCACCGAACCG
TTCGATCCGCCGGCGGTGCTCGGTGAGCTGGTCCGGACGCGGCCACTGGCCCGGATGGTG
TATCCGGACGGTCACGTCGGCTGGCTGGCCACCAGCCACGAGCTCGCGCGTGAGGTGCTC
GGCAGCCAGGCGTTCAGCCACCGCTTCCACACCACACACGCCCCGGTGACGAAGAAGGGC
GAGATCTTCCCCTCCGTGCCCATCGTGCCGGGCGGGTTCATCCACATGGATCCGCCGGAG
CACACCCGCTACCGGAATCTGCTCAGCAGCGAGTTCAGTGCCCGCGCCACCGCGGCGTAC
GCCCCGCGGGTGGCCGAGCTGGCCGCCGCGCAGCTCGCCCAGGTACGGCAGCACGGCTCA
CCGGTCGACCTCCTGCCGACGTACGTCCGGCCGCTCTCGCTGCGGGTGTTGTGCGAGGTG
CTGGGGGTCCCGTTCACCGGCAGCCCGGTCCTGTCCGCTCTGAGCGACAGCGCCACCGAC
GACGAGGTCAGCCTCGAGGCGGAGCGGGAGGCCGGGACCCGGGCGTACCACTACCTCCTG
GACCTCGTGGGCAAGGCACGGGTCGAGCCGGGCGAGAACGTCACCGGGCGGTTGGCCGCC
GACCCCGAGCTGACCGACCGCGAGATCACCAACATGCTGCTGATCGTCTTCGCGGCGGGC
TTCGCCAGCTGCGAGGGCGCGCTCGCGTCGTCGTTGCTGGCGCTGCTGCACCACCGCGAC
CAGCTCGACAGGCTGCGTTCGGCCGCCCGGAGCGGCGGCGCCGACGGGATCGAGAAAATG
GTCGAGGAGCTGCTGCGGTACACCTCGGTCAACCAGTTCCAGATCTTCCGGACCGCACTG
TCCGATGTGGAACTCGGCGGGGAACTGGTCCGCGAGGGGGAGACGGTGACGGTCGCCTTG
CCGGCGGCCAACCGTGATCCGGCCAGGTTCGGCTGTCCCGACGGGCTGGATGTGAGCCAG
GACGCTTCCGCCCACCTGGCCTTCGGATACGGCGTACACGCCTGCGTCGGACAGCGGCTG
GCCCGGCTCGTCCTGGCCGAGGGGCTGACCGCGCTGGTGCTCGGCTTCCCCGGACTCGCC
CTGGACGTGCCGCTGCGGGAGGTTCCGGTACGGCTGAAGACCCCGGTGCTGGGCGTACTG
CGCCTGCCCGTGCGCTACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) G3DSA:1.10.630.10 Cyt_P450 SSF48264 Cytochrome_P450 PF00067 p450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

Rubra_00280 Rubra_00300