Rubra_00450 Rubra_00450   Rubra_00470 Rubra_00470

Rubra_00460 : CDS information

close this sectionLocation

Organism
StrainNRRL 3061 (=NBRC 14000)
Entry nameRubradirin
Contig
Start / Stop / Direction86,106 / 87,158 / + [in whole cluster]
86,106 / 87,158 / + [in contig]
Location86106..87158 [in whole cluster]
86106..87158 [in contig]
TypeCDS
Length1,053 bp (350 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.2 modification addition of starter unit
Productputative aminodehydroquinate synthase
Product (GenBank)putative aDHQ synthase
Gene
Gene (GenBank)rubG
EC number4.2.3.-
Keyword
  • AHBA
Note
Note (GenBank)
Reference
ACC
PmId
[18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008)
comment
Rubradirin生合成clusterの報告。

Table1>配列比較からの推定機能
RubG(350aa): aDHQ synthase

rifamycin産生株で見つかっているAHBA biosynthetic genes, rifK, L, M, N, G, H, and Jの産物と高度な相同性を示す（rubNを除く）6つの遺伝子がrubradirin生合成gene clusterにある。
rubG and rubHは転写的にリンクされている。
Related Reference
ACC
O52549
NITE
Rifam_00280
PmId
[11278540] Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699. (J Biol Chem. , 2001)
comment
3rd, 76%, 1e-141
Amycolatopsis mediterranei_rifG(aroB)
3-dehydroquinate synthase(EC 4.2.3.4)
[DoBISCUIT]putative aminodehydroquinate synthase

rifamycinと関連ansamycinsのPKS starter 3-amino-5-hydroxybenzoic acid (AHBA)の生合成経路について調査。関連遺伝子の不活化、異種性発現、中間体を基質とした反応確認など。

RifG: Aminodehydroquinate synthase

rifH, -G, and -J genesの遺伝子産物は shikimate biosynthesis pathwayに関連する酵素に似ている。rifH, G, Jの各mutantは成長に影響を与えないので、shikimate pathwayに直接関連しないことがわかる。また、mutantでもrifamycin B産生をある程度保つことから、shikimate pathwayのgeneが代替していると考えられる。

AHBA biosynthetic genesをプラスミドに組み込んだS. coelicolor YU105を使ってAHBA生産を調べると、rifGを除いた場合はAHBA biosynthetic genesが全て揃っている場合と比較して、AHBAの生産量が約10%にまで落ちることが示されており、rifGはAHBAの生産に関与していることが示されている。
ACC
P0A4Z4
PmId
[17586643] Functional characterization by genetic complementation of aroB-encoded dehydroquinate synthase from Mycobacterium tuberculosis H37Rv and its heterologous expression and purification. (J Bacteriol. , 2007)
[1910148] The Mycobacterium tuberculosis shikimate pathway genes: evolutionary relationship between biosynthetic and catabolic 3-dehydroquinases. (Mol Gen Genet. , 1991)
comment
86th(P0A4Z4) 46%, 8e-51
Mycobacterium tuberculosis_aroB
3-dehydroquinate synthase(EC 4.2.3.4)

[PMID: 17586643]
Mycobacterium tuberculosis H37Rv_aroBをクローニング、E. coliで大量発現してhomogeneityに精製、アミノ酸シークエンス。M. tub_aroBはE. coli aroB deletion mutantを相補することを確認。

close this sectionSequence

selected fasta
>putative aminodehydroquinate synthase [putative aDHQ synthase]
MTAVSLRLRDQSYEVIIGPGVRTALAGVVRRLGAQRAVVVSARPRPWVPDTGVPSLVLPA
LDGEQGKTLGTVESLCGEFARFGLTRSDVVVSCGGGTTTDVVGLAAALYHRGVPVVHLPT
SLLAQVDAGVGGKTAVNLPAGKNLVGTYWQPSAVLCDTDYLSTLPRREMLSGLGEIARCH
FIGAPDLRGLPLAEQITASVRLKARIVEADERDTGMRHLLNYGHTLGHAVERATGYAVRH
GEAVAIGTVFAGRLAGALGRIGQARVAEHHDVVRHYGLPAALPAEAGTAALMRLMRQDKK
AVRGLTFVLDGPKGAELVSDVPPDVVAGVLEGMPRTCMADLVASSGGAGR
selected fasta
>putative aminodehydroquinate synthase [putative aDHQ synthase]
ATGACCGCGGTTTCTCTGCGTCTGAGAGATCAGTCGTATGAGGTGATCATTGGTCCGGGG
GTGCGGACGGCGCTCGCCGGCGTCGTCCGGCGGCTGGGCGCCCAGCGAGCGGTCGTCGTG
TCGGCTCGCCCACGGCCATGGGTGCCCGACACCGGGGTGCCCAGCCTGGTGCTGCCCGCG
CTCGACGGCGAGCAGGGCAAGACCCTCGGTACGGTGGAGTCGTTGTGCGGTGAGTTCGCA
CGGTTCGGGCTGACCAGGTCCGACGTGGTGGTCTCGTGCGGCGGCGGTACCACCACGGAT
GTCGTCGGGCTGGCGGCCGCGCTGTACCACCGGGGCGTGCCGGTGGTTCATCTGCCGACC
TCCCTGCTGGCCCAGGTGGATGCCGGCGTCGGCGGGAAGACGGCGGTGAACCTGCCCGCC
GGCAAGAACCTGGTCGGCACGTACTGGCAGCCCAGCGCGGTGCTCTGCGACACTGACTAC
CTGTCGACCCTGCCGCGGCGGGAGATGCTCAGTGGCCTCGGCGAAATCGCCCGCTGCCAC
TTCATCGGGGCGCCGGACCTGCGGGGGCTGCCCCTGGCCGAGCAGATCACGGCCAGCGTG
CGGCTCAAGGCCCGCATCGTGGAGGCGGACGAGCGGGACACCGGGATGCGGCACCTGCTC
AACTACGGCCACACGCTGGGCCATGCGGTGGAGAGAGCGACGGGCTACGCGGTGCGGCAC
GGCGAGGCGGTGGCCATCGGAACGGTCTTCGCGGGACGGCTCGCCGGTGCTCTGGGCCGT
ATCGGGCAGGCCCGGGTGGCAGAGCATCACGACGTCGTCCGGCATTACGGGCTTCCCGCC
GCACTGCCGGCCGAGGCCGGCACCGCCGCCCTGATGCGGTTGATGCGGCAGGACAAGAAG
GCGGTCCGGGGACTCACCTTCGTCCTGGACGGCCCCAAGGGCGCGGAGCTGGTGAGCGAC
GTCCCGCCGGATGTGGTCGCGGGGGTGCTGGAGGGGATGCCGCGCACGTGCATGGCCGAC
CTCGTCGCTTCGTCGGGTGGGGCTGGTCGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR016037 3-dehydroquinate synthase AroB (Family)
[2-338] 1.50000965748778e-79 PIRSF001455
PIRSF001455   DHQ_synth
SignalP
[1-34] 0.156 Signal
Bacteria, Gram-negative   
[1-34] 0.101 Signal
Eukaryota   
TMHMM No significant hit
Rubra_00450 Rubra_00450   Rubra_00470 Rubra_00470
Page top