Rubra_00510 Rubra_00510   Rubra_00530 Rubra_00530

Rubra_00520 : CDS information

close this sectionLocation

Organism
StrainNRRL 3061 (=NBRC 14000)
Entry nameRubradirin
Contig
Start / Stop / Direction93,240 / 94,229 / + [in whole cluster]
93,240 / 94,229 / + [in contig]
Location93240..94229 [in whole cluster]
93240..94229 [in contig]
TypeCDS
Length990 bp (329 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductNDP-hexose C-4 ketoreductase
Product (GenBank)putative 4-Ketoreductase
Gene
Gene (GenBank)rubN6
EC number
Keyword
  • D-rubranitrose
Note
Note (GenBank)
Reference
ACC
PmId
[18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008)
[16614891] Functional characterization of ketoreductase (rubN6) and aminotransferase (rubN4) genes in the gene cluster of Streptomyces achromogenes var. rubradiris. (Biotechnol Lett. , 2006)
comment
[PMID: 18080113](2008)
Rubradirin生合成clusterの報告。

Table1>配列比較からの推定機能
RubN6(329aa): 4-Ketoreductase

RubN1-N8はdTDP-D-rubranitroseの合成に推定的に関与していることが配列解析で明らかになった。

---
上記論文でrubN4, N6のrefとして挙げられているもの。
[PMID: 16614891](2006) abstract
rubN6 and rubN4をHis-tagged fusion proteinsとしてE.coliで異種性に発現。
それぞれのdTDP-glucose 4-ketoreductase and dTDP-glucose 3-aminotransferaseの機能性を、in vitro enzyme assayによって検証。

close this sectionSequence

selected fasta
>NDP-hexose C-4 ketoreductase [putative 4-Ketoreductase]
MTAAGDGGHRPLIVVLGGSGFIGAAVVEALARRPVRLRTVGRRPQPVPAGAVADIETVCA
DLTGRAALEAAVAGADTVVHLLMHTADWREAAGNPAAEEVNVGVMRDLVGLLGNTRRPPA
VLFAGSTSQAGLPERMPIDGSEPDRPTTAYDRHKHTAEQILKAADAAGLVRGVSLRLPTV
YGDSPRGRLTGRGVVCTMVRKAFAGEELTLWNDGTVERDLLYVHDAADAFLAGIDHAQVL
AGRHWLLGTGRGVPLGDVLRTIADLVGAHLGRPPVPVVCLPPPDFATATDMHSVTIDSSR
FRAATGWRPRVPLHLALRRCVEAFAHSAR
selected fasta
>NDP-hexose C-4 ketoreductase [putative 4-Ketoreductase]
GTGACGGCCGCGGGGGACGGCGGTCACCGGCCGCTGATCGTGGTGCTGGGCGGTTCGGGC
TTCATCGGCGCCGCAGTCGTCGAAGCCCTCGCCCGGCGCCCGGTCCGGCTGCGCACCGTC
GGCCGCCGCCCGCAGCCGGTGCCGGCCGGCGCGGTGGCCGACATCGAGACGGTGTGCGCC
GACCTCACCGGCCGCGCCGCGCTCGAAGCGGCGGTGGCCGGCGCGGACACGGTGGTCCAT
CTGCTGATGCACACCGCCGACTGGCGTGAGGCCGCGGGCAACCCGGCCGCCGAAGAAGTG
AACGTCGGTGTGATGCGCGACCTCGTCGGCCTGCTCGGCAACACCCGCCGGCCGCCCGCC
GTCCTGTTCGCGGGCTCCACCTCGCAGGCCGGCCTGCCCGAGCGGATGCCCATCGACGGC
AGCGAACCCGACCGGCCCACGACCGCCTACGACCGGCACAAGCACACAGCCGAGCAGATA
CTCAAGGCCGCCGACGCCGCCGGGCTGGTCCGCGGCGTCTCCCTGCGGCTGCCCACCGTG
TACGGGGACAGCCCGCGGGGACGGCTGACCGGCCGCGGCGTGGTCTGCACCATGGTGCGC
AAGGCTTTCGCCGGCGAGGAACTCACCCTGTGGAACGACGGCACCGTCGAACGCGACCTG
CTGTACGTGCACGACGCCGCGGACGCCTTCCTCGCCGGGATCGACCACGCCCAGGTGCTC
GCGGGCCGGCACTGGCTGCTGGGCACCGGCCGCGGCGTGCCCCTGGGCGATGTCCTGCGG
ACGATCGCCGACCTGGTCGGCGCCCACCTGGGGCGGCCGCCGGTGCCCGTCGTGTGCCTG
CCGCCGCCCGACTTCGCCACGGCCACCGACATGCACAGCGTCACGATCGACTCCTCAAGG
TTCCGGGCGGCCACCGGCTGGCGCCCCCGGGTGCCGCTGCACCTCGCGCTGCGGCGCTGC
GTCGAGGCGTTCGCCCACAGCGCCCGATAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[13-248] 2.80000000000001e-45 PF01370
PF01370   Epimerase
IPR016040 NAD(P)-binding domain (Domain)
[13-203] 2.3e-26 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-31] 0.767 Signal
Eukaryota   
[1-24] 0.215 Signal
Bacteria, Gram-negative   
TMHMM
[12-31] Transmembrane (i-o)
Transmembrane 1   
Rubra_00510 Rubra_00510   Rubra_00530 Rubra_00530
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