Rubra_00530 : CDS information

Location

Organism	Streptomyces achromogenes var. rubradiris
Strain	NRRL 3061 (=NBRC 14000)
Entry name	Rubradirin
Contig	AJ871581
Start / Stop / Direction	95,616 / 94,240 / - [in whole cluster] 95,616 / 94,240 / - [in contig]
Location	complement(94240..95616) [in whole cluster] complement(94240..95616) [in contig]
Type	CDS
Length	1,377 bp (458 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase
Product (GenBank)	putative TDP-glucose-2,3-dehydratase
Gene
Gene (GenBank)	rubN3
EC number
Keyword	D-rubranitrose
Note
Note (GenBank)
Reference	ACC Q2PC37 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) comment Rubradirin生合成clusterの報告。 Table1>配列比較からの推定機能 RubN3(458aa): TDP-glucose-2,3-dehydratase RubN1-N8はdTDP-D-rubranitroseの合成に推定的に関与していることが配列解析で明らかになった。
Related Reference	ACC Q845B8 PmId [12889805] Functional identification of rub52 gene involved in the biosynthesis of rubradirin. (Biotechnol Lett. , 2003) comment 2nd, 100%, 0.0 Streptomyces achromogenes_rub52 DTDP-glucose-2,3-dehydratase Q2PC37と同じrubradirin産生株NRRL3061での配列だが、position356でのC→Wの1aaのみ異なる。 Streptomyces achromogenes var. nibradiris NRRL3061のrub52を、E.coliで可溶性His-tagged fusion proteinとして発現。in vitro enzyme assayからthymidine diphospho-4-keto-6-deoxyglucoseのC-2 deoxygenation機能性が割り当てられた。

Sequence

>putative dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase [putative TDP-glucose-2,3-dehydratase]
MTTATQLAARDDTRLPARLERSRRAVVAGTGMRTQDVDAWLAGRRQAHRFTVERVPLEKI
DGWSFEPGSGNVVHRSGRFFSVEGLDVAIDGRAWQQPIIVQPEVGILGILAKEFDGVLHF
LMQAKMEPGNPGLLQLSPTVQATYSNYTGVHRGADVKYLEYFTRPGRGRVVADVLQSEHG
SWFLCKSNRNMIIETFDEVRPDEDFCWLTLGQIGSLLRRDHVVNMDARSVLACAPMAPAE
AGAVRSDTDVLSWFTNERARHEVRARRIPLAEVSDWVRTEDHIRHVDNRYFRVGGVSVVA
GNREVTSWMQPLLEPVGVGLCAFLVRRFAGVVHLLVHARAEGGLLDTVELGPTVQCTPQN
YAHLPEREQPPFLDLVRRAGADRIRYAALHSDEGGRFHNAVSRCLLVAATEAEAGAEPPP
GFAWVTPGQLHSLARHGHYLNMQARTLLAVVGLLEGLL

>putative dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase [putative TDP-glucose-2,3-dehydratase]
ATGACGACCGCGACACAGCTGGCAGCGCGGGATGACACCCGGCTGCCCGCACGGCTGGAG
CGTTCCCGGCGGGCCGTCGTGGCCGGTACGGGCATGCGGACCCAGGACGTCGACGCCTGG
CTCGCCGGGCGCCGCCAGGCGCACCGCTTCACCGTCGAGCGGGTGCCGCTTGAGAAGATC
GACGGCTGGTCGTTCGAACCGGGAAGCGGCAATGTCGTGCACCGCAGCGGGCGGTTCTTC
AGCGTGGAGGGGCTGGATGTCGCGATCGACGGCCGGGCGTGGCAGCAGCCGATCATCGTG
CAGCCCGAGGTGGGGATCCTCGGCATTCTGGCCAAGGAGTTCGACGGTGTCCTGCACTTT
TTGATGCAGGCCAAGATGGAGCCCGGTAATCCCGGCCTGCTGCAGCTCTCGCCGACCGTG
CAGGCCACCTACAGCAATTACACCGGTGTGCACCGGGGCGCCGATGTGAAGTACCTGGAG
TACTTCACGCGGCCGGGAAGGGGCCGGGTGGTCGCCGATGTGCTGCAGTCGGAGCACGGT
TCCTGGTTTTTGTGCAAGTCCAACCGCAACATGATCATCGAGACGTTCGACGAGGTGCGG
CCGGACGAGGACTTCTGCTGGCTCACGCTGGGACAGATCGGCAGCCTGCTGCGCCGTGAC
CATGTCGTGAACATGGACGCGCGCAGTGTCCTGGCCTGTGCGCCGATGGCGCCCGCGGAG
GCGGGCGCGGTGCGCTCGGACACCGATGTGCTGTCGTGGTTCACCAACGAGCGGGCCCGG
CACGAGGTGCGGGCCCGGCGTATCCCGCTGGCCGAGGTGAGCGACTGGGTGCGCACCGAG
GACCACATCCGCCACGTCGACAACCGGTATTTCCGGGTCGGCGGGGTGTCCGTCGTCGCC
GGCAACCGTGAGGTGACCTCGTGGATGCAGCCGCTGCTGGAGCCGGTCGGCGTGGGTTTG
TGCGCGTTCCTGGTCCGCCGCTTCGCCGGGGTGGTGCACCTGCTGGTCCACGCCCGGGCG
GAGGGGGGCCTCCTCGACACCGTCGAACTCGGGCCGACGGTGCAGTGCACCCCGCAGAAC
TATGCCCATCTGCCGGAGCGGGAGCAGCCGCCGTTCCTTGATCTGGTGCGCCGTGCGGGA
GCGGACCGGATCCGCTACGCGGCGCTGCACTCGGACGAGGGCGGCCGTTTCCACAACGCG
GTCAGCCGCTGCCTGCTGGTGGCGGCCACCGAGGCGGAGGCGGGTGCCGAGCCGCCGCCC
GGGTTCGCCTGGGTCACTCCCGGCCAGCTGCATTCGCTGGCCCGGCATGGCCACTACCTG
AACATGCAGGCGCGTACGCTGCTCGCGGTCGTGGGGCTGCTGGAGGGGCTGTTGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	No significant hit
TMHMM	No significant hit

Rubra_00520 Rubra_00540